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Plasmid#216533PurposeAn axon-targeted jGCaMP8m controlled by hSynapsin1 promoter and flex switchDepositorInsertaxon-jGCaMP8m
UseAAV and Cre/LoxTagsGAP43ExpressionMammalianMutationPromoterhSynapsin1Available sinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCL055 AAVS1-9xTetO-UbC-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA
Plasmid#188747PurposeDonor vector for integrating UbC promoter-driven dual surface marker/mCitrine fluorescent reporter at AAVS1 locusDepositorInsertSurface marker and mCitrine reporter
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterUbCAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJT055 AAVS1-BSD-9xTet0-pEF-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA
Plasmid#187324PurposeDonor to install a dual surface marker - fluorescence reporter into the AAVS1 safe harbor using HDRDepositorInsertBSD-9xTet0-pEF-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA
UseCRISPR and TALENTagsExpressionMammalianMutationPromoterpEF1aAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1-V5-HisB-ObKHC-wt-HaloTag
Plasmid#201551PurposeExpresses wild-type Octopus kinesin-1 motor with HaloTag in Drosophila S2 cellsDepositorInsertKinesin-1 (partial)
UseTagsHaloTagExpressionInsectMutationCodon-optimized for Drosophila expressionPromoterAc5Available sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB_CRFmut
Plasmid#208659PurposeExpresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) control sensor GRAB_CRFmut in neuronsDepositorInsertGPCR activation based corticotropin-releasing factor (CRF) contorl sensor GRAB_CRFmut
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceOct. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
LMAPPW
Plasmid#205657PurposeshRNAmir backbone for RNA interference, with Puromycin selectionDepositorInsertmiR-AB
UseLentiviralTagsExpressionMutationPromotermCMVAvailable sinceSept. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
TagBFP2-PIP4K2AD273K
Plasmid#202727Purposeexpresses fluorescent protein-tagged enzymeDepositorInsertmTagBFP2:PIP4K2A(D266K) (PIP4K2A Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
TagBFP2- PIP4K2BD278K
Plasmid#202729Purposeexpresses fluorescent protein-tagged enzymeDepositorInsertmTagBFP2:PIP4K2B (D278K) (PIP4K2B Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
TagBFP2- PIP4K2CD280K
Plasmid#202731Purposeexpresses fluorescent protein-tagged enzymeDepositorInsertmTagBFP2:PIP4K2C (D280K) (PIP4K2C Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGLDV_41 [11o1G]
Plasmid#199070PurposeOdd assembly vector with sfGFP dropout for Odd level assembliesDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCK074 p5E exorh:EGFP
Plasmid#195951PurposeGateway p5E vector with reporterDepositorInsertexorh:EGFP
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTC392
Plasmid#91223Purposeprotoplast vector expressing gRNA23 targeting tomato ANT1 (control without TREX2 expression)DepositorInsertgRNA targeting tomato ANT1
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSLfa[Tc'hsp5'3'UTR]fa
Plasmid#86450PurposeHeat shock-inducible promoter including hsp-UTRs, ubiquitous expression upon heat-shock of any inserted gene of interest in Tribolium; (Shuttle plasmid)DepositorInsertTc'hsp5'3'UTR
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
IMS-APEX2
Plasmid#79058PurposeExpresses APEX in the mitochondrial intermembrane space.DepositorInsertAPEX
UseTagsV5ExpressionBacterial and MammalianMutationPromoterT7 promoterAvailable sinceAug. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
AA030
Plasmid#216010PurposeFragmid fragment: (Cas protein) nickase Cas for base editingDepositorHas ServiceCloning Grade DNAInsertnCas9-SpG (D10A)_v1.1 [Sp]
UseCRISPR; FragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
Sybody Generation Toolbox
Plasmid Kit#1000000160PurposeThe Seeger Lab protocol to select synthetic nanobodies – called sybodies – against (membrane) protein targets. This collection contains all plasmids necessary for the sybody generation.DepositorAvailable sinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-E2-Crimson
Plasmid#197196PurposeExpresses the protein of E2-Crimson in mammalian cellsDepositorInsertE2-Crimson
UseAAVTagsExpressionMutationPromoterAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
Syn61
Bacterial Strain#174513PurposeRecoded E. coli strain without TCG, TCA, or TAG codons in all open reading frames. Full sequence - GenBank: CP040347.1DepositorBacterial ResistanceStreptomycinAvailable sinceNov. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS423-CUP1-His-Ubi
Plasmid#99541PurposeYeast episomal vector for expression of His-tagged ubiquitin; HIS3 markerDepositorInsertUBI3(1-76) (RPL40A Budding Yeast)
UseTags7xHisExpressionYeastMutationPromoterCUP1Available sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEJS2422_Nme2.Smu-ABE8e-i1
Plasmid#201520PurposeMammalian expression of domain inlaid Nme2.Smu-ABE8e-i1DepositorInsertNme2.Smu-ABE8e-i1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only