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pLV-EF1a-Itprip-V5

Plasmid

#120241

Purpose

Expresses V5-tagged Itprip in lentiviral vector

Depositor

Larry Gerace

Article

Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

Insert

Itprip (ITPRIP Human)

Use

Lentiviral

Tags

V5

Expression

Mutation

Promoter

EF1a

Available since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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RCAS-HA-b-actin

Plasmid

#125834

Purpose

Retroviral expression of HA-tagged human beta-actin

Depositor

Yi Li

Article

Toneff et al Neoplasia. 2010 Sep;12(9):718-26.

Insert

Beta-actin (ACTB Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

July 23, 2019

Availability

Academic Institutions and Nonprofits only

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pQCascade-IS186

Plasmid

#140627

Purpose

Expresses V. cholerae TniQ, Cas8, Cas7, and Cas6 and crRNA-IS186. The crRNA-IS186 targets IS186 loci in Escherchia coli.

Depositor

Sheng Yang

Article

Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

Insert

VchTniQ, VchCas8, VchCas7, VchCas6, crRNA-IS186

Use

CRISPR; Transposon

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1P DDX11_2

Plasmid

#160778

Purpose

Suppress DDX11

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shDDX11_2

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1P DDX11_1

Plasmid

#160777

Purpose

Suppress DDX11

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shDDX11_1

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pSI-535

Plasmid

#131130

Purpose

gRNA expression vector for C-to-T base editing reporter

Depositor

Nozomu Yachie

Article

Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

Insert

C-to-T reporter activation gRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Human U6 promoter

Available since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pET-Duet1-His-TEV-mCherry-OPTN WT

Plasmid

#190191

Purpose

Plasmid for the expression of His-TEV-mCherry-OPTN WT. Internal reference: SMC797

Depositor

Sascha Martens

Article

Zaffagnini et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

Insert

optineurin (OPTN Human)

Use

Tags

6xHis, TEV, and mCherry

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pv9_MBD1_MBD-WT-13XL-BASU

Plasmid

#179416

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MBD1.MBD-WT-13XL-BASU

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

MBD1.MBD-WT-13XL-BASU

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAGGS

Available since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pv9_TAF3_2xPHD-13XL-BASU

Plasmid

#179414

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of TAF3.2xPHD-13XL-BASU

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

TAF3.2xPHD-13XL-BASU

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAGGS

Available since

Oct. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pv9_CBX1_2xChromo-13XL-BASU

Plasmid

#179412

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX1.2xChromo-13XL-BASU

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

CBX1.2xChromo-13XL-BASU

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAGGS

Available since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pET28b-GBP-MTn

Plasmid

#154363

Purpose

Bacterial expression of MTn

Depositor

Wanzhong He

Article

Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

Insert

MTn

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pSc-His6-Sem1Sc

Plasmid

#83235

Purpose

pGREG600-His6-Sem1Sc, Expresses the His6-Sem1 in yeast

Depositor

Gali Prag

Article

Levin-Kravets et al Nat Methods. 2016 Nov;13(11):945-952. do

Insert

Sem 1 (SEM1 Budding Yeast)

Use

Tags

His6

Expression

Yeast

Mutation

Promoter

Available since

Nov. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pGLS3-5xHRE-PHLDA3(wt)

Plasmid

#72557

Purpose

Hypoxia induced mutant PHLDA3. Contains the HRE from VEGF sequence (5 repeats) upstream of PHLDA3.

Depositor

Ester Hammond

Article

Leszczynska et al J Clin Invest. 2015 Jun;125(6):2385-98.

Insert

PHLDA3 (PHLDA3 Human)

Use

Tags

5XHRE

Expression

Mammalian

Mutation

Promoter

E1b minimal

Available since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

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eSpCas9(1.1)_No_FLAG_ATP1A1_G3

Plasmid

#86611

Purpose

Expresses the ATP1A1 G3 sgRNA in combination with FLAGless eSpCas9(1.1) to target ATP1A1 intron 4. Px330-like plasmid

Depositor

Yannick Doyon

Article

Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n

Insert

ATP1A1 G3 sgRNA + FLAGless enhanced specificity Cas9 (1.1)

Use

CRISPR; Co-selection via hdr using ouabain

Tags

Expression

Mammalian

Mutation

K848A, K1003A, & R1060A

Promoter

CBh

Available since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

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Flag-M4-AR-S81D

Plasmid

#171242

Purpose

Mammalian expression of 3xFlag-tagged human androgen receptor phosphorylation mutant: AR-Ser81Asp (AR-S81D)

Depositor

Steven Balk

Article

Chen et al J Biol Chem. 2012 Mar 9;287(11):8571-83.

Insert

3xFlag-tagged human androgen receptor Ser81Asp mutant (AR Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

AR-Ser81Asp (AR-S81D)

Promoter

CMV

Available since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pUC19-E6MBW6

Plasmid

#103102

Purpose

Cas9 coding gene template with optimized sequence for human codon usage

Depositor

Duhee Bang

Article

Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

Insert

E6MBW6(Cas9 coding gene from Staphylococcus lugdunensis M23590)

Use

CRISPR; Cloning vector

Tags

Expression

Mutation

human codon-optimized

Promoter

Available since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pMXs-hs-3xHA-delta6xNHL-LIN-41

Plasmid

#52722

Purpose

retroviral expression of human LIN-41 / TRIM71 containing deletion of the 6xNHL repeats and 3 N-terminal HA tags

Depositor

Shinya Yamanaka

Article

Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

Insert

LIN-41 (TRIM71 Human)

Use

Retroviral

Tags

3xHA

Expression

Mammalian

Mutation

deleted amino acids 593–868

Promoter

Available since

June 11, 2014

Availability

Academic Institutions and Nonprofits only

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pLKO.1P POLD1_1

Plasmid

#160791

Purpose

Suppress POLD1

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shPOLD1_1

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO.1P REV3L_2

Plasmid

#160798

Purpose

Suppress REV3L

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shREV3L_2

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1P REV3L_1

Plasmid

#160797

Purpose

Suppress REV3L

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shREV3L_1

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

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