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Plasmid#79383PurposeCas9 gRNA variant to recruit ms2 proteinsDepositorInsertZhang 2.0 + scaffold
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJOE6089.4
Plasmid#135081PurposepBR322-based L-rhamnose-inducible vector.DepositorInsertP-rhaP-BAD-eGFP-Strep-tag II
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSPPac-hp
Plasmid#48123PurposeShuttle vector E. coli/B.meg.; encoding the signal peptide for protein secretion of the Bacillus megaterium protein Pac under control of the xylose inducible promoterDepositorInsertsignal peptide of Pac
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSPNprM-hp
Plasmid#48120PurposeShuttle vector E. coli/B.meg.; encoding the signal peptide for protein secretion of the Bacillus megaterium protein NprM under control of the xylose inducible promoterDepositorInsertsignal peptide of NprM
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSPVpr-hp
Plasmid#48119PurposeShuttle vector E. coli/B.meg.; encoding the signal peptide for protein secretion of the Bacillus megaterium protein Vpr under control of the xylose inducible promoterDepositorInsertsignal peptide of Vpr
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
TDP43 NOTAG11
Plasmid#28210DepositorInsertTar DNA binding protein (TARDBP Human)
UseTagsExpressionMammalianMutationaa193-274 onlyPromoterAvailable sinceSept. 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
acvr1l-VAAA-HA
Plasmid#201905Purposein vitro transcription of RNA encoding HA-tagged Acvr1l that contains 4 amino acid substitutions: T186V, S187A, S189A, S191ADepositorInsertacvr1l (acvr1l Zebrafish)
UseTagsHAExpressionMutationT186V, S187A, S189A, S191APromoterSP6Available sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
CMV-10xQb oligo-library based
Plasmid#158201PurposeCassette of 10 different binding sites with high affinity to QB phage coat protein, as extracted from the oligo pool experiment.DepositorInsert10 binding sites of QB based on oligo library experiment
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
Thy1.1-muSOX D219A
Plasmid#131704Purposeencodes Thy1.1 followed by a P2A site and muSOX D219A catalytic mutant for enriching pure populations of transfected cellsDepositorInsertThy1.1-muSOX
UseTagsExpressionMammalianMutationAspartic acid 219 mutated to an alaninePromoterCMVAvailable sinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-WNT1
Plasmid#79499PurposeMultiSite Gateway entry clones, second fragment (attL5, attL2) Human WNT1 CDSDepositorInsertWNT1 (WNT1 Human)
UseMultisite gateway entry cloneTagsExpressionMutationPromoterno PromoterAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSB700 lenti gRNA Cerulean-zhang2.0-Weiss-chimera2
Plasmid#79380PurposeCas9 gRNA variant to recruit ms2 proteinsDepositorInsertChimera 2
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPK1E-2
Plasmid#48146PurposeShuttle vector E. coli/B.meg., encoding a promoter for RNA polymerase of the phage K1EDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pPK1E-1
Plasmid#48145PurposeShuttle vector E. coli/B.meg., encoding a promoter for RNA polymerase of the phage K1EDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSB700 lenti gRNA Cerulean-zhang2.0-Weiss-chimera1
Plasmid#79379PurposeCas9 gRNA variant to recruit ms2 proteinsDepositorInsertChimera 1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pSB700 lenti gRNA Cerulean-zhang2.0-Weiss-chimera4
Plasmid#79382PurposeCas9 gRNA variant to recruit ms2 proteinsDepositorInsertChimera 4
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pCDNA5-LAP-MPS1-WT
Plasmid#114044PurposeExpression of exogenous LAP tagged MPS1 WTDepositorInsertMPS1-WT (TTK Human)
UseTagsExpressionMammalianMutationWT MPS1 sequencePromoterAvailable sinceSept. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSB700
Plasmid#64046PurposeLentiviral vector for expressing U6 sgRNA and CAGGS Cerulean fluorescent proteinDepositorTypeEmpty backboneUseCRISPR, Lentiviral, and Synthetic BiologyTagsExpressionMammalianMutationPromoterU6, CAGGSAvailable sinceMay 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pC5Kan-P2A
Plasmid#51814PurposeP2A peptide from porcine teschovirus-1 flanked by restriction sites in shuttle vector for easy cloning of multiple separate proteins to be expressed from single messageDepositorInsertP2A peptide (PTV1gp1 porcine teschovirus-1, Synthetic)
UseShuttle vector for insect expression vectorsTagsExpressionMutationcodons altered for Drosophila and to eliminate re…PromoterAvailable sinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
Mm-PylRS-AF/Pyl-tRNACUA
Plasmid#122650PurposetRNA synthetase/tRNA pair for the incorporation of unnatural amino acid into proteins in mammalian cells in response to the amber codon TAG.DepositorInsertPylRS-AF, Pyl-tRNACUA
UseTagsFLAGExpressionMammalianMutationTyrosine 306 changed to Alanine, Tyrosine 384 cha…PromoterAvailable sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
AnkyrinB-2XHA
Plasmid#31057DepositorInsertAnkyrin B (ANK2 Human)
UseTags2XHAExpressionMammalianMutationPromoterAvailable sinceMarch 5, 2012AvailabilityAcademic Institutions and Nonprofits only