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Plasmid#32521DepositorInsertDominant negative Rheb (D60I) (Rheb Rat)
UseLentiviralTagsExpressionMammalianMutationD60I to make dominant negativePromoterCMVAvailable sinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
p6900 PHAGE-P CMVT N-HA-hE6AP III C840A
Plasmid#37606DepositorInsertE6AP III C840A (UBE3A Human)
UseTagsHAExpressionMammalianMutationC840APromoterCMV tet regulatableAvailable sinceAug. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHAGE pGK LysoFlux (HaloTag7-mGFP-Galectin3)
Plasmid#215009PurposeExpress a Lysophagy reporter in mammalian cells (untested)DepositorInsertLGALS3 (LGALS3 Human)
UseLentiviralTagsHaloTag(7)-mGFPExpressionMammalianMutationPromoterAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
p2707-pHAGE-Ef1aL-dsRed-UBC-tagBFP
Plasmid#216476Purposelentiviral dual expression of dsRed and tagBFPDepositorInsertsdsRed
tagBFP
UseLentiviralTagsExpressionMutationPromoterEF1aL and UBCAvailable sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-EF1a-4xGFP11-SEC61B-IRES-Hygro
Plasmid#211468PurposeLentivirus backbone expressing SEC61B protein tagged with 4 tandem GFP11 repeats at the N-terminalDepositorInsert4xGFP11-SEC61B
UseLentiviralTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHAGE FL NAP1 STOP C' EGFP IRES Blast
Plasmid#179102PurposeExpresses untagged full length NAP1DepositorInsertNak associated protein 1 (AZI2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-NLS-dMisCas13b-SNAPf-NLS-3xFlag
Plasmid#191536PurposeOverexpression of NLS-dMisCas13b-SNAPf-NLS-3xFlag in human cellsDepositorInsertNLS-dMisCas13b-SNAPf-NLS-3xFlag
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHage2-EF1a-TERT K78E-IRES-Puro
Plasmid#182591Purposeexpresses TERT K78E in mammalian cellsDepositorInserthTERT (TERT Human)
UseLentiviralTagsExpressionMammalianMutationK78EPromoterEF-1aAvailable sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE UbiC NLS-HA-stdMCP-stdHalo-RH1
Plasmid#164614PurposeLentiviral vector expressing synonymous tandem MS2 coat protein fused to synonymous tandem Halo-tag and the RH1 domain of RILPL2. Used for single-molecule imaging of actin-anchored mRNA.DepositorInsertstdMCP-stdHalo-RH1
UseLentiviralTagsHA tag and NLSExpressionBacterial and MammalianMutationPromoterUbiCAvailable sinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPHAGE-EF1α-Luc-C20orf24-3’UTR-VAR
Plasmid#128507PurposeLentiviral constitutive expression of Firefly luciferase under control of 3'UTR of human C20orf24 with variant from COX deficiency patient.DepositorInsertFirefly luciferase
UseLentiviral and LuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
phage - ubc - nls - 2xmcp - egfp - cMycBirA*
Plasmid#131133PurposeBirA* expressed as a protein fusion with MS2 coat protein (MCP) dimer and EGFP; to visualize any MS2 tagged mRNAs and to identify the RNA proximal proteome by their biotinylation.DepositorInsertNLS-HA-2xMCP-EGFP-cMycBirA*
UseLentiviralTagsNLS, HA, cMyc (N-terminal of BirA*)ExpressionMammalianMutationBirA* = BIrA >R118GPromoterhuman ubiquitin C (UBC)Available sinceAug. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-TetOminiCMV-Sox2-17-t2a-Klf4-e2a-Myc
Plasmid#193349PurposetetO-S*KM (Dox-inducible reprogramming lentiviral vector expressing mouse super-Sox + Klf4 + cMyc)DepositorUseLentiviralTagsExpressionMammalianMutationSox2-17 is engineered highly cooperative chimeric…PromoterTetO mini CMVAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTS96 pHAGE2 CMVtetO2 GFP-22xGS-SUMOEu-MCS
Plasmid#199349PurposeLentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-SUMOEu-tagged protein in mammalian cells.DepositorTypeEmpty backboneUseLentiviralTagsEGFP-SUMOEuExpressionMammalianMutationWTPromoterAvailable sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTS106 pHAGE2 CMVtetO2 MCS-3C-SUMOEu-22xGS-GFP
Plasmid#199359PurposeLentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-SUMOEu-GFP-tagged protein in mammalian cells.DepositorTypeEmpty backboneUseLentiviralTags3C-SUMOEu-EGFPExpressionMammalianMutationWTPromoterAvailable sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-TetOminiCMV-Oct4-P2A-Klf4-IRES-Sox2-17
Plasmid#210020PurposetetO-OKS* (Dox-inducible reprogramming lentiviral vector expressing mouse Oct4 + Klf4 + super-Sox)DepositorUseLentiviralTagsExpressionMammalianMutationPromoterTetO-mini-CMVAvailable sinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
p2711-pHAGE2-Ef1aL-TAZ4SA-UBC-tagBFP-loxP
Plasmid#216480Purposelentiviral dual expression of TAZ4SA and tagBFP with loxP site for Cre excisionDepositorInsertsUseCre/Lox and LentiviralTagsExpressionMutationS66A, S89A, S117A, S311APromoterEF1aL and UBCAvailable sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dPspCas13b-2xmNeongreen-3xFlag
Plasmid#132403Purposeoverexpression in human cellsDepositorInsertdPspCas13b
UseLentiviralTagsflagExpressionMammalianMutationH133A; H1059APromoterAvailable sinceJan. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTS95 pHAGE2 CMVtetO2 GFP-22xGS-TEV-MCS
Plasmid#199348PurposeLentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-TEV-tagged protein in mammalian cells.DepositorTypeEmpty backboneUseLentiviralTagsEGFP-TEVExpressionMammalianMutationWTPromoterAvailable sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
p2709-pHAGE2-Ef1aL-YAP5SA-UBC-tagBFP-loxP
Plasmid#216478Purposelentiviral dual expression of YAP5SA and tagBFP with loxP site for Cre excisionDepositorInsertsYAP5SA
tagBFP
UseCre/Lox and LentiviralTagsExpressionMutationS61A, S109A, S127A, S164A, S381APromoterEF1aL and UBCAvailable sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
p2710-pHAGE2-Ef1aL-wtYAP-UBC-tagBFP-loxP
Plasmid#216479Purposelentiviral dual expression of wtYAP (YAP1) and tagBFP with loxP site for Cre excisionDepositorInsertsUseCre/Lox and LentiviralTagsExpressionMutationPromoterEF1aL and UBCAvailable sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only