Skip to main content
Addgene
Showing: 981 - 1000 of 2436 results

  1. V37m

    Plasmid
    #121043
    Purpose
    MoClo golden gate assembly AE destination vector DVCK-AE-mScar (ColE1/kan resistance destination vector with constitutive mScarlet expressing for screening.
    Depositor
    Richard Murray
    Insert
    Kan destination vector with ColE1 origin and mScarlet selection
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121043-plasmid-map-sequence-id-236355

    Enlarge

  2. C83m

    Plasmid
    #120994
    Purpose
    MoClo golden gate assembly CD part for pir (Initiation factor required for replication of R6K origin plasmids). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    pir protein required for R6K ori
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120994-plasmid-map-sequence-id-236103

    Enlarge

  3. C79m

    Plasmid
    #120991
    Purpose
    MoClo golden gate assembly CD part for ccdA (Antitoxin to ccdB/C39m. Sequence taken from pMAR7 plasmid sequence). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    ccdA antitoxin
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120991-plasmid-map-sequence-id-236074

    Enlarge

  4. C24m

    Plasmid
    #120964
    Purpose
    MoClo golden gate assembly CD part for lambda cI (repressor of pL or pOR1-OR2; use C64m for temperature-sensitive version). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    lambda-cI
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120964-plasmid-map-sequence-id-236100

    Enlarge

  5. C27m

    Plasmid
    #120965
    Purpose
    MoClo golden gate assembly CD part for TetR (tet repressor; identical to C2d but without ssrA degradation tag). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    tetR (no deg tags)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120965-plasmid-map-sequence-id-236387

    Enlarge

  6. C98m

    Plasmid
    #121002
    Purpose
    MoClo golden gate assembly CD part for Gp2 (RNA polymerase inhibitor from T7 phage; lethal to host when expressed). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    Gp2 toxin
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121002-plasmid-map-sequence-id-236135

    Enlarge

  7. C51m

    Plasmid
    #120975
    Purpose
    MoClo golden gate assembly CD part for sfYFP (superfolder YFP; variant of https://www.fpbase.org/protein/yfp/ - Please see Supplemental Documents for annotated Genbank file
    Depositor
    Richard Murray
    Insert
    sfYFP (has BbsI site)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120975-plasmid-map-sequence-id-236029

    Enlarge

  8. C33m

    Plasmid
    #120968
    Purpose
    MoClo golden gate assembly CD part for AiiA (AHL degradase; see doi: [10.1126/science.aaa3794]). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    AiiA degradase (Bennett, no ssra tag)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120968-plasmid-map-sequence-id-238044

    Enlarge

  9. P57m

    Plasmid
    #120949
    Purpose
    MoClo golden gate assembly AB part for pCin* (Adam Meyer's improved pCin promoter from https://doi.org/10.1101/285866, selected for dynamic range and less cross-sensitivity).
    Depositor
    Richard Murray
    Insert
    pCin Adam Meyer
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120949-plasmid-map-sequence-id-235961

    Enlarge

  10. L1P139 - pSPCH-PhiC31-tUBQ1

    Plasmid
    #219703
    Purpose
    Level 1 pSPCH-PhiC31-tUBQ1, for early stage of stomata development expression of PhiC31 integrase
    Depositor
    Jennifer Nemhauser
    Insert
    pSPCH:PhiC31:tUBQ1
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    June 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    219703-plasmid-map-sequence-id-435733

    Enlarge

  11. L0-T15 BpiI-p35s-BpiI

    Plasmid
    #219698
    Purpose
    Level 0 to insert p35S promoter to history-dependent target
    Depositor
    Jennifer Nemhauser
    Insert
    BpiI-p35s-BpiI
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    219698-plasmid-map-sequence-id-435729

    Enlarge

  12. pBD026

    Plasmid
    #201309
    Purpose
    AvrSr27-2 as Golden Gate compatible MoClo CDS part
    Depositor
    Benjamin Schwessinger
    Insert
    AvrSr27-2
    Use
    Tags
    Expression
    Plant
    Mutation
    removal of BsaI and BpiI sites
    Promoter
    Available since
    Oct. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    201309-plasmid-map-sequence-id-410982

    Enlarge

  13. pBD025

    Plasmid
    #201308
    Purpose
    AvrSr27-1 as Golden Gate compatible MoClo CDS part
    Depositor
    Benjamin Schwessinger
    Insert
    AvrSr27-1
    Use
    Tags
    Expression
    Plant
    Mutation
    removal of BsaI and BpiI sites
    Promoter
    Available since
    Oct. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    201308-plasmid-map-sequence-id-409510

    Enlarge

  14. T18m

    Plasmid
    #121037
    Purpose
    MoClo golden gate assembly DE part for L3S2P21 (382x-strength synthetic terminator with BsaI cut site removed; see DOI: 10.1038/nmeth.2515).
    Depositor
    Richard Murray
    Insert
    modified L3S2P21 (synthetic Voigt terminator)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121037-plasmid-map-sequence-id-236351

    Enlarge

  15. V35m

    Plasmid
    #121042
    Purpose
    MoClo golden gate assembly AE destination vector DVCK-AE-GFP (ColE1/kan resistance destination vector with constitutive GFP expression for screening.
    Depositor
    Richard Murray
    Insert
    Kan destination vector with ColE1 origin and GFP selection
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121042-plasmid-map-sequence-id-247704

    Enlarge

  16. U17m

    Plasmid
    #121023
    Purpose
    MoClo golden gate assembly BC part for Bujard RBS (strong RBS from https://doi.org/10.1093/nar/25.6.1203). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    Bujard RBS
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121023-plasmid-map-sequence-id-245898

    Enlarge

  17. U19m

    Plasmid
    #121024
    Purpose
    MoClo golden gate assembly BC part for MG-aptamer (fluorescent RNA aptamer of malachite green; see DOI: 10.1021/ja0529788) - Please see Supplemental Documents for annotated Genbank file
    Depositor
    Richard Murray
    Insert
    MG-apt
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121024-plasmid-map-sequence-id-238066

    Enlarge

  18. T15m

    Plasmid
    #121034
    Purpose
    MoClo golden gate assembly DE part for L3S3P11 (173x-strength synthetic terminator, modified to remove BsaI cut site; see DOI: 10.1038/nmeth.2515).
    Depositor
    Richard Murray
    Insert
    modified L3S3P11 (synthetic Voigt terminator)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121034-plasmid-map-sequence-id-245426

    Enlarge

  19. C85m

    Plasmid
    #120996
    Purpose
    MoClo golden gate assembly CD part for sigmaX (Bacillus subtilis RNA polymerase involved in heat response and biofilm formation). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    Sigma X (ECF20_992)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120996-plasmid-map-sequence-id-236125

    Enlarge

  20. C91m

    Plasmid
    #120999
    Purpose
    MoClo golden gate assembly CD part for sfCFP (superfolder CFP developed by Ye Chen in Matthew Bennet's lab) - Please see Supplemental Documents for annotated Genbank file
    Depositor
    Richard Murray
    Insert
    sfCFP (Chen, Bennett)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120999-plasmid-map-sequence-id-236129

    Enlarge

Showing: 981 - 1000 of 2436 results