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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 3,761 results for: golden gate

Showing: 981 - 1000 of 3761 results

  1. pAV10

    Plasmid
    #63213
    Purpose
    pUC19 backbone (Amp), contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. NotI or FseI sites for TU digest.
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Availability
    Academic Institutions and Nonprofits only
    63213-plasmid-map-sequence-id-113915

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  2. pENTR_AtU6p_BsaI_ccdb_gRNA

    Plasmid
    #225986
    Purpose
    To make Gateway entry clone of CRISPR guide RNA under AtU6p
    Depositor
    Imran Siddiqi
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available Since
    Oct. 7, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    225986-plasmid-map-sequence-id-448026

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  3. pRDA_887

    Plasmid
    #245329
    Purpose
    Cas12a CRISPRa cloning vector; contains NbALFA, p65
    Depositor
    John Doench, David Root
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    None
    Promoter
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    245329-plasmid-map-sequence-id-488027

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  4. pLlacO1-DP6

    Plasmid
    #242138
    Purpose
    AraC compatible Mutagenesis Circuit (drift plasmid)
    Depositor
    Dominik Niopek
    Insert
    Gene III
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Sept. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    242138-plasmid-map-sequence-id-484679

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  5. pDest_PANCEd_AraC_sd2_WT

    Plasmid
    #242148
    Purpose
    Expression Plasmid containing a LacI repressed sd2 WT AraC cassette
    Depositor
    Dominik Niopek
    Insert
    AraC
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Aug. 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    242148-plasmid-map-sequence-id-484657

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  6. pPSG804

    Plasmid
    #239534
    Purpose
    CRISPR plasmid with Cas9 and sgRNA targeting YDR514C locus in S. cerevisiae
    Depositor
    John Dueber
    Insert
    sgRNA_YDR514C
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    July 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    239534-plasmid-map-sequence-id-479034

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  7. pPSG779

    Plasmid
    #239532
    Purpose
    CRISPR plasmid withCas9 and sgRNA targeting YBR197C locus in S. cerevisiae
    Depositor
    John Dueber
    Insert
    sgRNA_YBR197C
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    239532-plasmid-map-sequence-id-478373

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  8. pJJB1434

    Plasmid
    #239494
    Purpose
    Express Idi1p and Erg20WW for synthesis of GPP in the peroxisome
    Depositor
    John Dueber
    Insert
    ScErg20p(F96W_N127W)-ePTS1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239494-plasmid-map-sequence-id-475871

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  9. pPSG803

    Plasmid
    #239533
    Purpose
    CRISPR plasmid with Cas9 and sgRNA targeting YMR206W locus in S. cerevisiae
    Depositor
    John Dueber
    Insert
    sgRNA_YMR206W
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239533-plasmid-map-sequence-id-475867

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  10. pBD016

    Plasmid
    #201307
    Purpose
    Sr27 as Golden Gate compatible MoClo CDS part
    Depositor
    Benjamin Schwessinger
    Insert
    Sr27
    Use
    Tags
    Expression
    Plant
    Mutation
    removal of BsaI and BpiI sites
    Promoter
    Available Since
    Jan. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    201307-plasmid-map-sequence-id-410032

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  11. pPD1159 pLink 9

    Plasmid
    #139247
    Purpose
    Vector that holds the linker for circuits with 9 TUs
    Depositor
    Joshua Leonard
    Insert
    Linker9
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Feb. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    139247-plasmid-map-sequence-id-325057

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  12. pPD1158 pLink 8

    Plasmid
    #139246
    Purpose
    Vector that holds the linker for circuits with 8 TUs
    Depositor
    Joshua Leonard
    Insert
    Linker8
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Feb. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    139246-plasmid-map-sequence-id-325056

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  13. pPD1157 pLink 7

    Plasmid
    #139245
    Purpose
    Vector that holds the linker for circuits with 7 TUs
    Depositor
    Joshua Leonard
    Insert
    Linker7
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Feb. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    139245-plasmid-map-sequence-id-325055

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  14. pPD1156 pLink 6

    Plasmid
    #139244
    Purpose
    Vector that holds the linker for circuits with 6 TUs
    Depositor
    Joshua Leonard
    Insert
    Linker6
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Feb. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    139244-plasmid-map-sequence-id-325054

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  15. pPD1155 pLink 5

    Plasmid
    #139243
    Purpose
    Vector that holds the linker for circuits with 5 TUs
    Depositor
    Joshua Leonard
    Insert
    Linker5
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Feb. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    139243-plasmid-map-sequence-id-325051

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  16. pPD1154 pLink 4

    Plasmid
    #139242
    Purpose
    Vector that holds the linker for circuits with 4 TUs
    Depositor
    Joshua Leonard
    Insert
    Linker4
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Feb. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    139242-plasmid-map-sequence-id-325049

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  17. pPD1153 pLink 3

    Plasmid
    #139241
    Purpose
    Vector that holds the linker for circuits with 3 TUs
    Depositor
    Joshua Leonard
    Insert
    Linker3
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Feb. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    139241-plasmid-map-sequence-id-325047

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  18. pPD1152 pLink 2

    Plasmid
    #139240
    Purpose
    Vector that holds the linker for circuits with 2 TUs
    Depositor
    Joshua Leonard
    Insert
    Linker2
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Feb. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    139240-plasmid-map-sequence-id-325023

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  19. pPD1151 pLink 1

    Plasmid
    #139239
    Purpose
    Vector that holds the linker for circuits with 1 TUs
    Depositor
    Joshua Leonard
    Insert
    Linker1
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Feb. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    139239-plasmid-map-sequence-id-325022

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  20. C9m

    Plasmid
    #120958
    Purpose
    MoClo golden gate assembly CD part for deCFP (cyan fluorescent protein) - Please see Supplemental Documents for annotated Genbank file
    Depositor
    Richard Murray
    Insert
    deCFP
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120958-plasmid-map-sequence-id-235973

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Showing: 981 - 1000 of 3761 results