Addgene

We narrowed to 5,112 results for: donor

Showing: 981 - 1000 of 5112 results

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pMOD_C3006

Plasmid

#91096

Purpose

Module C, Promoter: FMV 34S, Gene: GFP (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

GFP (+ BaeI site for GT donor cloning)

Use

Unspecified

Tags

Expression

Mutation

Promoter

FMV 34S

Available since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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pRB14

Plasmid

#52522

Purpose

expresses a myc-tagged version of hCas9 in Drosophila

Depositor

Klaus Foerstemann

Article

Bottcher et al Nucleic Acids Res. 2014 Apr 19.

Insert

S. pyogenes cas9 with humanized codon bias

Use

Tags

myc

Expression

Insect

Mutation

Promoter

tubulin

Available since

April 22, 2014

Availability

Academic Institutions and Nonprofits only

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pMK277 (mClover-NeoR)

Plasmid

#72793

Purpose

C-terminal tagging with mClover using CRIPSR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

mClover-Neo

Use

CRISPR

Tags

mClover

Expression

Mammalian

Mutation

Promoter

Available since

March 24, 2016

Availability

Academic Institutions and Nonprofits only

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mCherry-FKBP-SAC1-HLx8

Plasmid

#108136

Purpose

Recruitable SAC1 with extended linker

Depositor

Gerry Hammond

Article

Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

Insert

mCherry:FKBP1A(3-108):[GGSA]4GG:SACM1L(1-520):[EAAAR]8:SACM1L(521-587) (SACM1L Human)

Use

Tags

Expression

Mammalian

Mutation

SACM1L(1-520):[EAAAR]8:SACM1L(521-587)

Promoter

CMV

Available since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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pMK285 (Stag-3FLAG-Bsr)

Plasmid

#72801

Purpose

C-terminal tagging with Stag-3FLAG using CRIPSR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

Stag-3FLAG-Bsr

Use

CRISPR

Tags

Stag-3FLAG

Expression

Mammalian

Mutation

Promoter

Available since

March 24, 2016

Availability

Academic Institutions and Nonprofits only

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pRB17

Plasmid

#52527

Purpose

serves as template for PCR-mediated fusion of U6-promoter with sgRNA

Depositor

Klaus Foerstemann

Article

Bottcher et al Nucleic Acids Res. 2014 Apr 19.

Insert

Drosophila U6C promoter

Use

CRISPR; Blunt cloning kit

Tags

T7-promoter

Expression

Insect

Mutation

Promoter

Available since

April 14, 2014

Availability

Academic Institutions and Nonprofits only

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pMOD_C2813

Plasmid

#91092

Purpose

Module C, Promoter: PvUbi2, Gene: Csy4 (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Csy4 (+ BaeI site for GT donor cloning)

Use

CRISPR

Tags

Expression

Mutation

Promoter

PvUbi2

Available since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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pMOD_C2806

Plasmid

#91091

Purpose

Module C, Promoter: FMV 34S, Gene: Csy4 (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Csy4 (+ BaeI site for GT donor cloning)

Use

CRISPR

Tags

Expression

Mutation

Promoter

FMV 34S

Available since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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pJET-07-mNeonGreen-R11a

Plasmid

#179890

Purpose

Donor with homologous arms for Rab11a to knock in mNeonGreen

Depositor

Patrick Caswell

Article

Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

Insert

RAB11A, member RAS oncogene family (RAB11A Human)

Use

CRISPR; Donor for homologous based knock in

Tags

mNeonGreen

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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mCherry-FKBP-SAC1-HLx10

Plasmid

#108137

Purpose

Recruitable SAC1 with extended linker

Depositor

Gerry Hammond

Article

Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

Insert

mCherry:FKBP1A(3-108):[GGSA]4GG:SACM1L(1-520):[EAAAR]10:SACM1L(521-587) (SACM1L Human)

Use

Tags

Expression

Mammalian

Mutation

SACM1L(1-520):[EAAAR]10:SACM1L(521-587)

Promoter

CMV

Available since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR-ARF19

Plasmid

#83846

Purpose

Gateway donor vector for ARF19

Depositor

Jennifer Nemhauser

Article

Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

Insert

ARF19 (ARF19 Mustard Weed)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Oct. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pGFPmAID_htz-1

Plasmid

#174214

Purpose

Donor plasmid for tagging htz-1 with GFP::mAID

Depositor

Masato Kanemaki

Article

Negishi et al Genetics. 2021 Dec 2. pii: 6448232. doi:

Insert

GFP::mAID::htz-1 tagging donor

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

Available since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

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mCherry-FKBP-SAC1C389S-HLx8

Plasmid

#108138

Purpose

Recruitable inactive SAC1 with extended linker

Depositor

Gerry Hammond

Article

Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

Insert

mCherry:FKBP1A(3-108):[GGSA]4GG:SACM1LC389S(1-520):[EAAAR]8:SACM1L(521-587) (SACM1L Human)

Use

Tags

Expression

Mammalian

Mutation

SACM1LC389S(1-520):[EAAAR]8:SACM1L(521-587)

Promoter

CMV

Available since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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pJG493

Plasmid

#91197

Purpose

WDV replicon T-DNA for gene targeting in wheat scutella, no Cas9 control (gUbi1+gUbi8+donor)

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

gUbi1+gUbi8+donor

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221-RAP1

Plasmid

#84525

Purpose

This plasmid can be used as a donor for subcloning into Gateway destination vectors.

Depositor

Jorrit Enserink

Article

Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

Insert

RAP1 (RAP1 Budding Yeast)

Use

Gateway donor plasmid

Tags

Expression

Mutation

no stop codon

Promoter

none

Available since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pJG486

Plasmid

#91195

Purpose

WDV replicon T-DNA for gene targeting in wheat scutella, H840A single nickase (nTaCas9_H840A+gUbi1+donor)

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

nTaCas9_H840A+gUbi1+donor

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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pMOD_C4300

Plasmid

#91103

Purpose

Module C, Promoter: none, Gene: BaeI LacZ cassette for GT donor cloning, Terminator: none

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

BaeI LacZ cassette for GT donor cloning

Use

Unspecified

Tags

Expression

Mutation

Promoter

none

Available since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pJG484

Plasmid

#91194

Purpose

WDV replicon T-DNA for gene targeting in wheat scutella, H840A single nickase (nTaCas9_H840A+gUbi8+donor)

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

nTaCas9_H840A+gUbi8+donor

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221-rap1-K651R

Plasmid

#84528

Purpose

This plasmid can be used as a donor for subcloning into Gateway destination vectors.

Depositor

Jorrit Enserink

Article

Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

Insert

RAP1 (RAP1 Budding Yeast)

Use

Gateway donor plasmid

Tags

Expression

Mutation

K651R

Promoter

none

Available since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR-IAA17_no stop

Plasmid

#83855

Purpose

Gateway donor vector for IAA17 with no stop codon

Depositor

Jennifer Nemhauser

Article

Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

Insert

IAA17 (AXR3 Mustard Weed)

Use

Gateway donor vector

Tags

Expression

Mutation

no stop codon

Promoter

Available since

Oct. 7, 2016

Availability

Academic Institutions and Nonprofits only

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