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Plasmid#178869PurposeMammalian expression of a protein of interest fused to the C-terminus of PB1-AzamiGreen-FRB-TEVuncs-mTagBFP-HADepositorInsertPB1-AzamiGreen-FRB-TEVuncs(NEYLQFL)-mTagBFP2-HA
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
330-BFP-hCYP3A4-enhancer-R-sgRNA
Plasmid#176841PurposeTargeting human CYP3A4 proximal enhancer right boundary. sgRNA expressing cells could be FACS sorted by BFP expression.DepositorInsertHuman CYP3A4 proximal enhancer right boundary
UseTagsExpressionMammalianMutationPromoterU6Available sinceNov. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUFlip-floxed2A-KalTA4-; gcry1:BFP -2
Plasmid#173891PurposeDonor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld methodDepositorInsert2A-KalTA4; gcry1: BFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(hYAP1-Y1K)-PGKpuro2ABFP-W
Plasmid#163170PurposeLentiviral gRNA plasmid targeting human YAP1 gene, co-expression of BFP tagDepositorInsertYAP1 (YAP1 Human)
UseCRISPR and LentiviralTagsBFPExpressionMammalianMutationPromoterU6Available sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-mTagBFP2-T2A-iCreERT2 (PAAA)
Plasmid#137860PurposeMonomeric TagBFP2 and iCre-ERT2 recombinase (PAAA N-terminus)DepositorInsertmTagBFP2-T2A-iCreERT2
UseCre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceMarch 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-mTagBFP2-T2A-iCreERT2 (PAAAA)
Plasmid#137861PurposeMonomeric TagBFP2 and iCre-ERT2 recombinase (PAAAA N-terminus)DepositorInsertmTagBFP2-T2A-iCreERT2
UseCre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceMarch 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-mTagBFP2-T2A-iCreERT2 (PAGSA)
Plasmid#137862PurposeMonomeric TagBFP2 and iCre-ERT2 recombinase (PAGSA N-terminus)DepositorInsertmTagBFP2-T2A-iCreERT2
UseCre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceMarch 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-mTagBFP2-T2A-iCreERT2 (PAAGSA)
Plasmid#137863PurposeMonomeric TagBFP2 and iCre-ERT2 recombinase (PAAGSA N-terminus)DepositorInsertmTagBFP2-T2A-iCreERT2
UseCre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceMarch 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-mTagBFP2-T2A-iCreERT2 (PVV)
Plasmid#128172PurposeMonomeric TagBFP2 and iCre-ERT2 recombinase (PVV N-terminus)DepositorInsertmTagBFP2-T2A-iCreERT2
UseCre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceMarch 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-mTagBFP2-T2A-iCreERT2 (PVGSA)
Plasmid#128173PurposeMonomeric TagBFP2 and iCre-ERT2 recombinase (PVGSA N-terminus)DepositorInsertmTagBFP2-T2A-iCreERT2
UseCre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceMarch 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Lrrc40-trunc-IRES-BFP
Plasmid#117268PurposeRetroviral overexpression of Lrrc40 - truncated version with seemingly same DE genes in Th2 as MSCV-Lrrc40-IRES-BFPDepositorInsertLrrc40 (Lrrc40 Mouse)
UseRetroviralTagsExpressionMammalianMutationThe insert is truncated - deletion of bp 334-407 …PromoterAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6(gDmap1 v4)-PGK-Puro-BFP
Plasmid#117143PurposeExpress gRNA vs Dmap1DepositorInsertgDmap1 v4 (Dmap1 Synthetic)
UseCRISPR and LentiviralTagsExpressionMutationDeleted BbsI site within WPRE, modified BFPPromoterhuman U6 promoter, mouse PGK promoterAvailable sinceApril 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6(gDmap1 v5)-PGK-Puro-BFP
Plasmid#117144PurposeExpress gRNA vs Dmap1DepositorInsertgDmap1 v5 (Dmap1 Synthetic)
UseCRISPR and LentiviralTagsExpressionMutationDeleted BbsI site within WPRE, modified BFPPromoterhuman U6 promoter, mouse PGK promoterAvailable sinceApril 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6(gDmap1 v1)-PGK-Puro-BFP
Plasmid#117140PurposeExpress gRNA vs Dmap1DepositorInsertgDmap1 v1 (Dmap1 Synthetic)
UseCRISPR and LentiviralTagsExpressionMutationDeleted BbsI site within WPRE, modified BFPPromoterhuman U6 promoter, mouse PGK promoterAvailable sinceApril 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6(gDmap1 v2)-PGK-Puro-BFP
Plasmid#117141PurposeExpress gRNA vs Dmap1DepositorInsertgDmap1 v2 (Dmap1 Synthetic)
UseCRISPR and LentiviralTagsExpressionMutationDeleted BbsI site within WPRE, modified BFPPromoterhuman U6 promoter, mouse PGK promoterAvailable sinceApril 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEJS592-pCSDest2-AcrIIC4Hpa-BFPv2-IRES
Plasmid#113438PurposeExpresses type II-C anti-CRISPR protein from H. parainfluenzae and a BFP from IRES in mammalian cellsDepositorInsertsType II-C anti-CRISPR
mTagBFP2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEJS593-pCSDest2-AcrIIC5Smu-BFPv2-IRES
Plasmid#113439PurposeExpresses type II-C anti-CRISPR protein from S. muelleri and a BFP from IRES in mammalian cellsDepositorInsertsType II-C anti-CRISPR
mTagBFP2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-multi-v2-mTagBFP2-NLS-BsmBI_entry (pRW1506)
Plasmid#225754PurposeEntry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with nuclear-localized mTagBFP2-NLS; v2 is compatible with T7 in vitro transcription detectionDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-multi-v2-palmitoyl-mTagBFP2-BsmBI_entry (pRW1509)
Plasmid#225755PurposeEntry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with membrane-localized palmitoyl-mTagBFP2; v2 is compatible with T7 in vitro transcription detectionDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKE12-ISceI-fabp10a:loxP-BFP-loxP-Xla.Ctnnb1
Plasmid#198240PurposeFor I-SceI-mediated transgenesis in zebrafish; fabp10a promoter drives expression of activated β-catenin preceded by a blue fluorescent protein (BFP)-STOP cassette flanked by loxP sitesDepositorInsertsUseZebrafish transgenesisTagsExpressionMutationPromoterAvailable sinceJuly 3, 2024AvailabilityAcademic Institutions and Nonprofits only