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pcDNA3.1-CHRM3-cpHalo∆-mEGFP

Plasmid

#194678

Purpose

CMV driven expression of the m3 GPCR recorder in mammalian cell lines fused to mEGFP

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

CHRM3_cpHalo∆

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-Myc-Oct3/4-IP

Plasmid

#13460

Depositor

Shinya Yamanaka

Article

Maruyama et al J Biol Chem. 2005 Jul 1. 280(26):24371-9

Insert

Oct3/4 (Pou5f1 Mouse)

Use

Tags

Myc (6x)

Expression

Mammalian

Mutation

None

Promoter

Available since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only

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pFRLuc-CoV2-FLucStop

Plasmid

#177617

Purpose

Dual luciferase reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting. In-frame stop codon inserted after FLuc (negative control).

Depositor

Junjie Guo

Article

Sun et al Proc Natl Acad Sci U S A. 2021 Jun 29;11

Insert

SARS-CoV-2 FSE

Use

Luciferase

Tags

Expression

Mammalian

Mutation

In-frame stop codon added between FLuc and FSE

Promoter

Available since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-FRT-Rev-3xGFP

Plasmid

#191204

Purpose

FlpO dependent GFP expression

Depositor

Janelia Viral Tools

Article

Winnubst et al Cell. 2019 Sep 19;179(1):268-281.e13. do

Insert

3X GFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-FLT3-V5/HIS

Plasmid

#201985

Purpose

expression of human FLT3 receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

FLT3 receptor tyrosine kinase (FLT3 Human)

Use

Tags

V5, 6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 21, 2023

Availability

Academic Institutions and Nonprofits only

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pL0M-S-mNeonGreen-EC18153

Plasmid

#137075

Purpose

Golden Gate Level 0 S module for N-terminal fluorescent protein tagging

Depositor

Julian Hibberd

Article

Luginbuehl et al Plant Direct. 2020 Feb 11;4(2):e00188. d

Insert

mNeonGreen DNA synthesis product with 5' and 3' extensions for Golden Gate cloning

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 12, 2020

Availability

Academic Institutions and Nonprofits only

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pKLV2-EF1a-BsdCas9-W

Plasmid

#67978

Purpose

Lentiviral vector expressing Cas9 fused with the Blasticidin resistant gene at the N-terminus

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

EF1a-Cas9 cassette, WPRE

Use

CRISPR and Lentiviral

Tags

Cas9 fused with Flag-tag and a NLS at the N termi…

Expression

Mutation

Promoter

EF1a

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pAT9650-BEAR-GFP-preedited

Plasmid

#162992

Purpose

BEAR control plasmid with split EGFP and intact 5' splice site

Depositor

Ervin Welker

Article

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Insert

EGFP split with an intron between amino acids 95-96

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 21, 2021

Availability

Academic Institutions and Nonprofits only

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LV-U6-sgRNAfiller-PGK-Cre-EFS-mScarletSIIN

Plasmid

#172436

Purpose

Lentivirus, expresses Cre recombinase and mScarlet-SIINFEKL, with sgRNA cloning site

Depositor

Tyler Jacks

Article

Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

Inserts

Cre
mScarlet-SIINFEKL(OVA257-264)+Ova 323-339

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-6p1-GST-ArcΔCTD

Plasmid

#119878

Purpose

Bacterial Expression construct for the production and purification of Arc missing C-terminal Domain

Depositor

Jason Shepherd

Article

Pastuzyn et al Cell. 2018 Mar 22;173(1):275. doi: 10.10

Insert

Activity Regulated Cytoskeletal Protein (Arc Rat)

Use

Tags

GST

Expression

Bacterial

Mutation

Deletion of nt 832-1110

Promoter

tac

Available since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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pET21b-Caspase-3(C163S)

Plasmid

#90088

Purpose

Expresses caspase 3 with C163S to prevent autocatalysis in bacterial cells

Depositor

Clay Clark

Article

Pop et al Biochemistry. 2001 Nov 27;40(47):14224-3

Insert

Caspase 3 (CASP3 Human)

Use

Tags

His tag

Expression

Bacterial

Mutation

C163S

Promoter

Available since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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pGST-Vps4

Plasmid

#21495

Depositor

James Hurley

Article

Wollert et al Nature. 2009 Mar 12. 458(7235):172-7.

Insert

VPS4 (VPS4 Budding Yeast)

Use

Tags

GST

Expression

Bacterial

Mutation

none

Promoter

Available since

Sept. 9, 2009

Availability

Academic Institutions and Nonprofits only

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pCAG-Oct3/4-IRES-Neo

Plasmid

#13461

Depositor

Shinya Yamanaka

Article

Tokuzawa et al Mol Cell Biol. 2003 Apr . 23(8):2699-708

Insert

Oct3/4 (Pou5f1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

P31S

Promoter

Available since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO V5 DNAJB2a

Plasmid

#19523

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

Insert

DNAJB2a (DNAJB2 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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IMPT-10314:GABBR2

Plasmid

#157851

Purpose

HA-3xFLAG-VFT-TMD-CC in pFastBac1 for Sf9 expression

Depositor

Vadim Cherezov, Raymond Stevens

Article

Shaye et al Nature. 2020 Jun 17. pii: 10.1038/s41586

Insert

GABBR2 (GABBR2 Human)

Use

Tags

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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6xHis(TEV)-Drp1-StrepII

Plasmid

#174422

Purpose

Expresses human Drp1 Isoform 3 in bacteria

Depositor

Thomas Pucadyil

Article

Kamerkar et al Nat Commun. 2018 Dec 7;9(1):5239. doi: 1

Insert

Drp1

Use

Tags

6xHis(TEV) and StrepII

Expression

Bacterial

Mutation

None

Promoter

T7

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pFRLuc-CoV2-delSS

Plasmid

#177615

Purpose

Dual luciferase reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting. Slippery sequence (SS) deleted (negative control).

Depositor

Junjie Guo

Article

Sun et al Proc Natl Acad Sci U S A. 2021 Jun 29;11

Insert

SARS-CoV-2 FSE

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Slippery sequence deleted

Promoter

Available since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pSGEMKv10.1

Plasmid

#85704

Purpose

Xenopus laevis oocyte expression of Kv10.1

Depositor

Luis Pardo

Article

Jenke et al EMBO J. 2003 Feb 3;22(3):395-403.

Insert

KCNH1 (KCNH1 Human)

Use

Xenopus expression

Tags

Expression

Mutation

Promoter

Available since

Dec. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO V5 DNAJB2b

Plasmid

#19524

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

Insert

DNAJB2b (DNAJB2 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 7, 2008

Availability

Academic Institutions and Nonprofits only

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pMy-BirA-T2A-eGFP

Plasmid

#50565

Purpose

Retroviral vector encoding for GFP and the biotinylating enzyme BirA

Depositor

Rafael Casellas

Article

Nakahashi et al Cell Rep. 2013 May 30;3(5):1678-89. doi:

Insert

Ampicilin

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 23, 2015

Availability

Academic Institutions and Nonprofits only

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