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Showing: 81 - 100 of 649 results

  1. pfMDH_N300S

    Plasmid
    #67729
    Purpose
    Expresses N300S mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli
    Depositor
    Justin Siegel
    Article
    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:
    Insert
    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    Use
    Tags
    6x-His tag
    Expression
    Bacterial
    Mutation
    Changed asparagine 300 to serine
    Promoter
    T7
    Available since
    Sept. 9, 2015
    Availability
    Academic Institutions and Nonprofits only

  2. pBBR-Cas9ΔyqhD-HA500

    Plasmid
    #220369
    Purpose
    A single-plasmid-based, easily cured CRISPR/Cas9 system for genome engineering in Pseudomonas putida KT2440
    Depositor
    Yilan Liu
    Inserts
    SpCas9
    sgRNA for yqhD in P. putida KT2440
    Use
    CRISPR; Broad host range
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    220369-plasmid-map-sequence-id-437258

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  3. pTA7002-avrPto

    Plasmid
    #49156
    Purpose
    Plant transformation binary vector for Dexamethasone inducible expression of the type III effector gene avrPto
    Depositor
    Sheng Yang He
    Article
    Hauck et al Proc Natl Acad Sci U S A. 2003 Jul 8;100
    Insert
    avrPto
    Use
    Plant transformation binary vector
    Tags
    Expression
    Mutation
    Promoter
    dexamethasone inducible
    Available since
    April 14, 2014
    Availability
    Academic Institutions and Nonprofits only

  6. pfMDH_Triple

    Plasmid
    #67733
    Purpose
    Expresses H303AR373AK381A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli
    Depositor
    Justin Siegel
    Article
    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:
    Insert
    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    Use
    Tags
    6x-His tag
    Expression
    Bacterial
    Mutation
    Changed histidine 303 to alanine, arginine 373 to…
    Promoter
    T7
    Available since
    Sept. 9, 2015
    Availability
    Academic Institutions and Nonprofits only

  13. pCas9-beta-mmr

    Plasmid
    #169241
    Purpose
    Cas9, beta, and mutL mutant expression plasmid for introducing chromosomal point mutations
    Depositor
    Brian Pfleger
    Article
    Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:
    Inserts
    cas9
    beta
    mutL-E36K
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Changed glutamic acid 36 to lysine
    Promoter
    Available since
    June 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    169241-plasmid-map-sequence-id-333575

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  15. pGLU_65

    Plasmid
    #225768
    Purpose
    This is the native promoter from the aac(3)-Ia gentamicin resistance cassette in IDT backbone
    Depositor
    Nili Ostrov
    Insert
    Paac(3)-Ia_[abr]
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    N/A
    Promoter
    Available since
    Sept. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    225768-plasmid-map-sequence-id-447050

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Showing: 81 - 100 of 649 results