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Plasmid#107596PurposeExpresses V5-APEX2-NES in mammalian cellsDepositorInsertAPEX2-NES
UseFrt site to insert into hek293 t-rex genomeTagsNES and V5ExpressionMammalianMutationPromoterCMV Dox inducibleAvailable sinceMay 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
IF701: pMVP (L5-L4) APEX2
Plasmid#121725PurposepMVP L5-L4 entry plasmid, contains APEX2 for 4-component MultiSite Gateway Pro assembly. Allows fusion of N-term APEX2 to gene of interest.DepositorInsertAPEX2 (open)
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pME-APEX2-mCherry
Plasmid#188944PurposeMiddle entry vector for Gateway cloning containing APEX2 tagged to mitochondria and nuclei, and cytoplasmic mCherryDepositorInsertmito-APEX2_p2A_APEX2-H2B_p2A_mCherry
UseMiddle entry vector for gateway (l1-l2)TagsExpressionMutationPromoterAvailable sinceOct. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRibo-Sec-APEX2
Plasmid#111699PurposeTheophylline-inducible expression of periplasmic APEX2 from a mycobacterial shuttle plasmid (episomal)DepositorInsertAPEX2
UseTagsV5ExpressionBacterialMutationPromoterhsp60-riboAvailable sinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMV306-ribo-APEX2
Plasmid#111700PurposeTheophylline-inducible expression of APEX2 from a mycobacterial shuttle plasmid (integrating)DepositorInsertAPEX2
UseTagsV5ExpressionBacterialMutationPromoterhsp60-riboAvailable sinceSept. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRSET-His-APEX2
Plasmid#201471PurposeRecombinant soluble APEX2 for SLAPSHOT labelingDepositorInsertAPEX2
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-APEX2-nDEST
Plasmid#214992PurposeGateway compatible empty vector (lentiviral/mammalian expression) with an N-terminal APEX2 tagDepositorTypeEmpty backboneUseLentiviralTagsFlag-APEX2ExpressionMammalianMutationPromoterAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-cDEST-APEX2
Plasmid#214993PurposeGateway compatible empty vector (lentiviral/mammalian expression) with a C-terminal APEX2 tagDepositorTypeEmpty backboneUseLentiviralTagsAPEX2-FlagExpressionMammalianMutationPromoterAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
SITS-MmCav1-APEX2
Plasmid#170526PurposeVector expression of MmCav1-APEX2 in Leishmania cell free system. Parton lab clone IYWDepositorInsertCaveolin1 (Cav1 Mouse)
UseLeishmania cell free expression cloneTagsAPEX2 and LifeactExpressionMutationPromoterAvailable sinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
Lifeact-mEos2-APEX2
Plasmid#115563PurposeLifeact-mEos2-APEX2 fusion for PALM and CLEM. Parton lab clone IETDepositorInsertmEos2
UseTagsAPEX2 and LifeactExpressionMammalianMutationPromoterAvailable sinceSept. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
p3E-APEX2 (GC rich)
Plasmid#115550PurposeMultisite gateway vector for 3' tagging with GC-rich APEX2. Used to make 115563. Parton lab clone HHSDepositorInsertAPEX2 (GC rich)
UseMultisite gateway entry cloneTagsExpressionMutationPromoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLX304-Flag-APEX2-NES
Plasmid#92158PurposeCytosolic APEX2 constructDepositorInsertFlag-APEX2-NES
UseLentiviralTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceAug. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-FLAG-APEX2-TAX1BP1
Plasmid#175761PurposeLentiviral vector expressing N-terminally tagged APEX2-FLAG-TAX1BP1DepositorInsertTAX1BP1 (TAX1BP1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-FLAG-APEX2-GFP
Plasmid#175757PurposeLentiviral vector expressing N-terminally tagged APEX2-FLAG-GFP (Control for APEX experiments)DepositorInsertGFP
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-FLAG-APEX2-OPTN
Plasmid#175760PurposeLentiviral vector expressing N-terminally tagged APEX2-FLAG-OPTNDepositorInsertOPTN (OPTN Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLJC5-3XHA-EGFP-PEX26
Plasmid#139054PurposeHA-PEROXO Lentiviral Construct. Expresses a Peroxisomal Tag.DepositorInsert3XHA-EGFP-PEX26
UseLentiviralTagsHAExpressionMutationPromoterUBCAvailable sinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEJS578_DD-dSpyCas9-mCherry-APEX2
Plasmid#108570PurposeExpression of drug-inducible dSpyCas9-mCherry-APEX2 for biotin-labeling genomic element-associated protein factorsDepositorInsertdSpyCas9
UseCRISPR and LentiviralTagsAPEX2, Destabilization domains, Flag, and mCherry…ExpressionMammalianMutationPromoterCMVAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLJC5-3XMyc-EGFP-PEX26
Plasmid#139059PurposeMyc-PEROXO Lentiviral Construct. Expresses a Peroxisomal Tag.DepositorInsert3XMyc-EGFP-PEX26
UseLentiviralTagsMycExpressionMutationPromoterUBCAvailable sinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Connexin43-GFP-APEX2
Plasmid#49385PurposeExpresses a tandem GFP-APEX2 fusion to the C-terminus of connexin43 in mammalian cellsDepositorInsertConnexin43 - EGFP - APEX2
UseTagsExpressionMammalianMutationK14D, W41F, E112K, A134P on soybean APX; improved…PromoterCMVAvailable sinceNov. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
Flag-APEX2-C Cloning Vector
Plasmid#128146PurposeTo express a protein of interest fused to the C-terminus of Flag-APEX2 for proximity labeling or TEMDepositorTypeEmpty backboneUseTagsFlag-APEX2ExpressionMammalianMutationPromoterAvailable sinceJuly 2, 2019AvailabilityAcademic Institutions and Nonprofits only