Skip to main content
Addgene
Showing: 81 - 100 of 211 results

  8. pMS2M-PH-gagpol-D64V

    Plasmid
    #166031
    Purpose
    Expression of bacteriophage-derived MS2 coat protein fused HIV-1 Lentiviral Gag-Pol at N terminal with a HIV-1 protease cleavage signal between MS2 and Gag-Pol.
    Depositor
    Yujia Cai
    Article
    Ling et al Nat Biomed Eng. 2021 Jan 4. pii: 10.1038
    Insert
    MS2-Gag-Pol
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    D64V mutation within the integrase within Pol
    Promoter
    Available since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    166031-plasmid-map-sequence-id-328290

    Enlarge

  10. pUBC_stdMCP_serinemod_E488QADAR_p2A_yGFP

    Plasmid
    #154787
    Purpose
    Expresses synonymized MS2 coat protein dimer (MCP) fused to hyperactive ADAR catalytic domain
    Depositor
    Robert Singer
    Article
    Biswas et al iScience. 2020 Jun 28;23(7):101318. doi:
    Insert
    Adenosine deaminse (Adar Fly, Synthetic)
    Use
    Lentiviral
    Tags
    GFP, V5, and stdMCP
    Expression
    Mammalian
    Mutation
    Synonymized serine to prevent autoediting of ADAR…
    Promoter
    UBC
    Available since
    July 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    154787-plasmid-map-sequence-id-306329

    Enlarge

  11. phage - ubc - nls - mcp - egfp - BirA*

    Plasmid
    #131134
    Purpose
    BirA* expressed as a protein fusion with MS2 coat protein (MCP) monomer and EGFP; to visualize any MS2 tagged mRNAs and to identify the RNA proximal proteome by their biotinylation.
    Depositor
    Ralf Jansen
    Insert
    NLS-HA-MCP-EGFP-BirA*
    Use
    Lentiviral
    Tags
    NLS, HA
    Expression
    Mammalian
    Mutation
    BirA* = BIrA >R118G
    Promoter
    human ubiquitin C (UBC)
    Available since
    Aug. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    131134-plasmid-map-sequence-id-255499

    Enlarge

  12. phage - ubc - nls - 2xmcp - egfp - cMycBirA*

    Plasmid
    #131133
    Purpose
    BirA* expressed as a protein fusion with MS2 coat protein (MCP) dimer and EGFP; to visualize any MS2 tagged mRNAs and to identify the RNA proximal proteome by their biotinylation.
    Depositor
    Ralf Jansen
    Insert
    NLS-HA-2xMCP-EGFP-cMycBirA*
    Use
    Lentiviral
    Tags
    NLS, HA, cMyc (N-terminal of BirA*)
    Expression
    Mammalian
    Mutation
    BirA* = BIrA >R118G
    Promoter
    human ubiquitin C (UBC)
    Available since
    Aug. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    131133-plasmid-map-sequence-id-255501

    Enlarge

  17. pUBC_mCherry_serinemod_E488QADAR_p2A_yGFP

    Plasmid
    #154786
    Purpose
    Expresses mCherry fused to hyperactive ADAR catalytic domain, control plasmid for HyperTRIBE
    Depositor
    Robert Singer
    Article
    Biswas et al iScience. 2020 Jun 28;23(7):101318. doi:
    Insert
    Adenosine deaminse (Adar Fly, Synthetic)
    Use
    Lentiviral
    Tags
    GFP, V5, and mCherry
    Expression
    Mammalian
    Mutation
    Synonymized serine to prevent autoediting of ADAR…
    Promoter
    UBC
    Available since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    154786-plasmid-map-sequence-id-306310

    Enlarge

  19. phage - ubc - nls - 2xmcp - egfp - BirA*

    Plasmid
    #131132
    Purpose
    BirA* expressed as a protein fusion with MS2 coat protein (MCP) dimer and EGFP; to visualize any MS2 tagged mRNAs and to identify the RNA proximal proteome by their biotinylation.
    Depositor
    Ralf Jansen
    Article
    Mukherjee et al Proc Natl Acad Sci U S A. 2019 Jun 25;11
    Insert
    NLS-HA-2xMCP-EGFP-BirA*
    Use
    Lentiviral
    Tags
    NLS, HA
    Expression
    Mammalian
    Mutation
    BirA* = BIrA >R118G
    Promoter
    human ubiquitin C (UBC)
    Available since
    Aug. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    131132-plasmid-map-sequence-id-255500

    Enlarge

  20. EK0409 SFFV-mCherry-SGc(s70587)cSG-EGFP (FLP-IN)

    Plasmid
    #191164
    Purpose
    Similar to EK0285 but has additional restriction sites around the sensor sequence. Recommended for cloning new sensors when the presence of MS2 sequences are not relevant (use EK0438 if MS2 sequences
    Depositor
    Xiaojing Gao
    Article
    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038
    Insert
    mCherry:s70587:EGFP (additional cutsites)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    SFFV
    Available since
    Oct. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    191164-plasmid-map-sequence-id-375473

    Enlarge

Showing: 81 - 100 of 211 results