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Plasmid#115790PurposeAn AAV vector expressing S. aureus dCas9 fused to VP64.DepositorInsertSa-dCas9
UseAAVTagsNLS and NLS-VP64ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-3xsgRNA_Opn1mw-RHO-Cas9N-IntN-polyA
Plasmid#165450PurposeExpress N-terminal part of split dCas9-VPR and 3 sgRNAs targeting murine Opn1mw promoterDepositorInsertsdCas9N-IntN
3x Opn1mw promoter-targeting sgRNAs
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterU6 and short human rhodopsin promoterAvailable sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
OA-986B
Plasmid#124999PurposeExpresses dCas9-VPR driven by the Ubiquitin-63E promoterDepositorInsertdCas9-VPR
UsePiggybackTagsExpressionInsectMutationPromoterUbiquitinAvailable sinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
OA-986C
Plasmid#125000PurposeExpresses dCas9-VPR driven by the bottleneck promoterDepositorInsertdCas9-VPR
UsePiggybackTagsExpressionInsectMutationPromoterbottleneckAvailable sinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEDJ-87
Plasmid#163085PurposeMarkerFree plasmid for integration of pADH1-MCP-VPR into site XI-5DepositorInsertVPR
UseTagsMCPExpressionYeastMutationPromoterADH1Available sinceSept. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT076
Plasmid#137880PurposeDonor vector for integration at the human AAVS1 safe harbor locus of Doxicycline-inducible dCas9-VPR-T2A-EGFPDepositorInsertTRE3G-dCas9-VPR-T2A-EGFP-CAG-TetON-NeoR-SA
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
JEH127
Plasmid#179299PurposeExpresses dCas9 fused to VPRDepositorInsertdCas9-VPR
UseTagsExpressionMammalianMutationD10A, H840A (catalytically inactive Cas9)PromoterCAGAvailable sinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pXPR_120
Plasmid#96917Purposefor CRISPRa, lentiviral expression of dCas9-VPR 2A BlastRDepositorInsertdCas9
UseLentiviralTagsVPRExpressionMammalianMutationD10A, N863APromoterEF1aAvailable sinceJune 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
GWF180
Plasmid#133607PurposeCAG-DNCR2-VPR-IRES-Puro-WPRE-SV40PA-PGK-NS3aH1-tagBFP-SpdCas9DepositorInsertDNCR2-VPR, NS3aH1-tagBFP-SpdCas9
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
ARB367
Plasmid#124050PurposeEncodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a expressing sadCas9 fused to VPR domain P2A mRuby2, and an sgRNA targeting pUAS expressing mAzamiGreen in a PiggyBac destination vectorDepositorInsertPB_pEF1a-tet3G-P2A-iRFP713_pEF1a-sadCAS9::VPR-P2A-mRuby2-SV40PA_mu6-sgUAS_pUAS-NLS::mAzamiGreen-rgPA
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
GB4068
Plasmid#187810PurposeModule for the constitutive expression of Cup2:Gal4AD and copper-inducible expression of dCasEV2.1 (dCas9:EDLL and MS2:VPR).DepositorInsertcopper-inducible dCasEV2.1
UseTagsExpressionPlantMutationPromoterAvailable sinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
GB2513
Plasmid#187803PurposeModule for constitutive expression of dCas9:EDLL, Ms2:VPR and a gRNA targeting the DFR promoter.DepositorInsertdCasEV2.1
UseTagsExpressionPlantMutationPromoterAvailable sinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
GB4070
Plasmid#187811PurposeModule for the constitutive expression of Cup2:Gal4AD and gRNA-1 DFR, and the copper-inducible expression of dCasEV2.1 (dCas9:EDLL and MS2:VPR).DepositorInsertcopper-inducible dCasEV2.1
UseTagsExpressionPlantMutationPromoterAvailable sinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRS156
Plasmid#182707PurposeCRISPRa plasmid for Candida albicans (dCas9-VPR and sgRNA cloning site)DepositorTypeEmpty backboneUseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMVP and pMAGIC Cloning Systems
Plasmid Kit#1000000155PurposepMVP and pMAGIC are cloning systems for adenoviral, lentiviral, expression, PiggyBac transposon, and Sleeping Beauty transposon vectors for transgene or RNAi delivery and dCas9-based engineering.DepositorAvailable sinceSept. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
Fungal Toolkit for Modular Cloning (FTK)
Plasmid Kit#1000000191PurposeConsists of 96 genetic parts as Modular Cloning-compatible entry vectors, including synthetic and native promoters, terminators, selection markers, and CRISPR components, for filamentous fungi.DepositorAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only