-
Plasmid#180642PurposeBacterial expression for CHMP4C C-terminal tail, residues 156-233. Used for FP competition experiments. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-14.DepositorInsertCHMP4C (CHMP4C Human)
UseTagsHIS-SUMOExpressionBacterialMutationresidues 316-366PromoterT7Available sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
GB1-ΔN-CHMP4C(S191C,I225A,L228A,W231A)
Plasmid#199242PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-CHMP4C(121-233, S191C,I225A,L228A,W231A)DepositorInsertGB1-His6-TEV-CHMP4C(121-233, S191C, I225A, L228A, W231A) (CHMP4C Human)
UseTagsGB1-His6-TEVExpressionBacterialMutationS191C, I225A, L228A, W231APromoterAvailable sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX2T-TEV CHMP1B 4-199 L188A/L192A
Plasmid#108289PurposeExpresses residues 4-199 with L to A mutations at residues 188 and 192 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-284DepositorInsertCHMP1B (CHMP1B Human)
UseTagsGSTExpressionBacterialMutationchanged L 188 to A, changed L 192 to APromoterAvailable sinceApril 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX2T-TEV CHMP1B 4-199 L192A/L195A
Plasmid#108291PurposeExpresses residues 4-199 with L to A mutations at residues 192 and 195 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-285DepositorInsertCHMP1B (CHMP1B Human)
UseTagsGSTExpressionBacterialMutationchanged L 192 to A, changed L 195 to APromoterAvailable sinceApril 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV(Δ3)-squirrel-monkey-full-length-CHMP3-HA
Plasmid#154169Purposeexpresses squirrel monkey CHMP3 in mammalian cellsDepositorInsertCHMP3
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV(Δ2)-squirrel-monkey-full-length-CHMP3-HA
Plasmid#154170Purposeexpresses squirrel monkey CHMP3 in mammalian cellsDepositorInsertCHMP3
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV(Δ1)-squirrel-monkey-full-length-CHMP3-HA
Plasmid#154171Purposeexpresses squirrel monkey CHMP3 in mammalian cellsDepositorInsertCHMP3
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEF1α-squirrel-monkey-full-length-CHMP3-HA
Plasmid#154172Purposeexpresses squirrel monkey CHMP3 in mammalian cellsDepositorInsertCHMP3
UseTagsHAExpressionMammalianMutationPromoterEF1-alphaAvailable sinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-OSF-PP-squirrel-monkey-CHMP3(155)
Plasmid#154183Purposeexpresses squirrel monkey CHMP3(155) in mammalian cellsDepositorInsertCHMP3(155)
UseTagsFLAG, Strep-Tag II, and preScission siteExpressionMammalianMutationC-terminal truncation of CHMP3 at amino acid 155PromoterAvailable sinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
GST-CHMP4A (205-222) (pGEX2T(TEV))
Plasmid#80633PurposeResidues 205-222 of CHMP4A, Bacterial Expression Vector; TEV-cleavable GST tag; Sundquist Lab Internal ID: WISP06-60DepositorInsertCHMP4A (CHMP4A Human)
UseTagsGSTExpressionBacterialMutationResidues 205-222;PromoterAvailable sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEF1α-squirrel-monkey-retroCHMP3-T2A-H2B-BFP
Plasmid#154185Purposeexpresses squirrel monkey retroCHMP3 with self-cleaving tag in mammalian cellsDepositorInsertretroCHMP3
UseTagsHA and T2A-H2B-BFPExpressionMammalianMutationPromoterEF1-alphaAvailable sinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
Swachmann-Bodian-Diamond Syndrome Gene, sbds_R (OZ546)
Plasmid#28071DepositorInsertZinc finger array targeting Swachmann-Bodian-Diamond Syndrome Gene, sbds (sbds Zebrafish)
UseZebrafish targetingTagsExpressionMutationPromoterAvailable sinceFeb. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
Swachmann-Bodian-Diamond Syndrome Gene, sbds_L (OZ545)
Plasmid#28070DepositorInsertZinc finger array targeting Swachmann-Bodian-Diamond Syndrome Gene, sbds (sbds Zebrafish)
UseZebrafish targetingTagsExpressionMutationPromoterAvailable sinceFeb. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
GST-CHMP4A (205-222) E209A (pGEX2T(TEV))
Plasmid#80640PurposeResidues 205-222 of CHMP4A, Mutation modestly disrupts binding to ALIX; Bacterial Expression Vector; TEV-cleavable GST-tag; Sundquist Lab Internal ID: WISP06-206DepositorInsertCHMP4A (CHMP4A Human)
UseTagsGSTExpressionBacterialMutationChanged Glutamate 209 to alaninePromoterAvailable sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX2T-TEV CHMP1B 186-199 L188A/L192A
Plasmid#108288PurposeExpresses residues 186-199 with L to A mutations at residues 188 and 192 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-286DepositorInsertCHMP1B (CHMP1B Human)
UseTagsGSTExpressionBacterialMutationchanged L 188 to A, changed L 192 to APromoterAvailable sinceApril 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX2T-TEV CHMP1B 186-199 L192A/L195A
Plasmid#108290PurposeExpresses residues 186-199 with L to A mutations at residues 192 and 195 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-287DepositorInsertCHMP1B (CHMP1B Human)
UseTagsGSTExpressionBacterialMutationchanged L 188 to A, changed L 192 to APromoterAvailable sinceApril 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
GST-CHMP4A (205-222) L217A (pGEX2T(TEV))
Plasmid#80638PurposeResidues 205-222 of CHMP4A, Mutation disrupts binding to ALIX; Bacterial Expression Vector; TEV-cleavable GST-tag; Sundquist Lab Internal ID: WISP06-204DepositorInsertCHMP4A (CHMP4A Human)
UseTagsGSTExpressionBacterialMutationResidues 205-222; Changed Leucine 217 to AlaninePromoterAvailable sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
GST-CHMP4A (205-222) W220A (pGEX2T(TEV))
Plasmid#80637PurposeResidues 205-222 of CHMP4A, Mutation disrupts binding to ALIX; Bacterial Expression Vector; TEV-cleavable GST-tag; Sundquist Lab Internal ID: WISP06-203DepositorInsertCHMP4A (CHMP4A Human)
UseTagsGSTExpressionBacterialMutationResidues 205-222; Changed Trp 220 to AlaPromoterAvailable sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
GST-CHMP4A (205-222) L214A (pGEX2T(TEV))
Plasmid#80639PurposeResidues 205-222 of CHMP4A, Mutation disrupts binding to ALIX; Bacterial Expression Vector; TEV-cleavable GST-tag; Sundquist Lab Internal ID: WISP06-205DepositorInsertCHMP4A (CHMP4A Human)
UseTagsGSTExpressionBacterialMutationResidues 205-222; Changed Leucine 214 to AlaninePromoterAvailable sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
Swachmann-Bodian-Diamond Syndrome Gene, sbds_R (OZ546)
Plasmid#28071DepositorInsertZinc finger array targeting Swachmann-Bodian-Diamond Syndrome Gene, sbds (sbds Zebrafish)
UseZebrafish targetingTagsExpressionMutationPromoterAvailable sinceFeb. 25, 2011AvailabilityAcademic Institutions and Nonprofits only