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Lentiviral expression of S. aureus dead Cas9 (dCas9/dSaCas9/SadCas9) fused to the KRAB domain as well as a gRNA targeting the tight TRE promoter and the blasticidin resistance gene.
Donor cassette plasmid for high-throughput tagging, encoding an mClover3 synthetic exon in frame 0, as well as a blasticidin resistance gene for selection of genomic integrants.
Donor cassette plasmid for high-throughput tagging, encoding an mClover3 synthetic exon in frame 2, as well as a blasticidin resistance gene for selection of genomic integrants.
Donor cassette plasmid for high-throughput tagging, encoding an mClover3 synthetic exon in frame 1, as well as a blasticidin resistance gene for selection of genomic integrants.
Donor cassette plasmid for high-throughput tagging, encoding an mNG2(11) synthetic exon in frame 0, as well as a blasticidin resistance gene for selection of genomic integrants.
Donor cassette plasmid for high-throughput tagging, encoding an mNG2(11) synthetic exon in frame 2, as well as a blasticidin resistance gene for selection of genomic integrants.
Donor cassette plasmid for high-throughput tagging, encoding an mNG2(11) synthetic exon in frame 1, as well as a blasticidin resistance gene for selection of genomic integrants.