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Plasmid#163855PurposeZebrafish muscle specific expression of mRuby2-dVHH[GFP]DepositorInsertdVHH[GFP]
UseIn vitro transcription of rnaTagsRuby2ExpressionMutationPromoterAvailable sinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVTU_260_BirA
Plasmid#63682PurposeEncodes E. coli biotin ligase gene (BirA) for expression in yeast cellsDepositorInsertBirA
UseTags6X Histidine and TEV cleavage siteExpressionYeastMutationPromoterADH1/yeastAvailable sinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET30b_T7Sav
Plasmid#138588PurposeT7-tagged streptavidin with native C-terminus controlled by PT7 promoter; KanR, pBR322 oriDepositorInsertT7-tagged streptavidin variant with native C-terminus
UseSynthetic BiologyTagsT7-tagExpressionBacterialMutationPromoterPT7Available sinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
p3E-TurboID
Plasmid#166570PurposeMultisite Gateway 3' entry clone for fusions onto the N-terminus of TurboID. Parton lab clone JSSDepositorInsertTurboID
UseMultisite gateway entry cloneTagsExpressionMutationPromoterAvailable sinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-TurboID-EGFP
Plasmid#163846PurposeIn vitro transcription of TurboID-EGFP. Parton lab clone JRSDepositorInsertTurboID
UseIn vitro transcription of rnaTagsEGFPExpressionMutationPromoterAvailable sinceSept. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pME-mKate2-P2A-TurboID-NS
Plasmid#166568PurposeMultisite Gateway middle entry clone encoding TurboID with mKate2 marker. Parton lab clone IYNDepositorInsertmKate2-P2A-TurboID
UseMultisite gateway entry cloneTagsExpressionMutationPromoterAvailable sinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBAD-BirA-eCPX-AP
Plasmid#121907PurposeExpresses BirA and eCPX-APDepositorInsertsUseTags6xHisExpressionBacterialMutationPromoterT7 and pBAD promoterAvailable sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-miniTurboID-EGFP
Plasmid#163847PurposeIn vitro transcription of miniTurboID-EGFP. Parton lab clone JRTDepositorInsertminiTurbo
UseIn vitro transcription of rnaTagsEGFPExpressionMutationPromoterAvailable sinceSept. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-NLS-TurboID
Plasmid#163849PurposeIn vitro transcription of nls-TurboID. Parton lab clone JSTDepositorInsertTurboID
UseIn vitro transcription of rnaTagsnlsExpressionMutationPromoterAvailable sinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUASp-Egl-Trbo
Plasmid#179993PurposeExpresses Egl fused at the C-terminus with TurboID. Expression is driven using the UAS-Gal4 system.DepositorInsertEgl-Trbo
UseTagsTurboIDExpressionInsectMutationPromoterUAS-Gal4Available sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pME-BASU-NS
Plasmid#166565PurposeMultisite Gateway middle entry clone encoding BASU. Parton lab clone JRODepositorInsertBASU
UseMultisite gateway entry cloneTagsExpressionMutationPromoterAvailable sinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-TurboID-CaaX
Plasmid#163848PurposeIn vitro transcription of TurboID-CaaX. Parton lab clone JSUDepositorInsertTurboID
UseIn vitro transcription of rnaTagsCaaXExpressionMutationPromoterAvailable sinceSept. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Tesmin-BioID2-3xFLAG
Plasmid#186815PurposeCAG promoter-driven expression of TESMIN-BioID2-3xFLAGDepositorInsertTesmin (Tesmin Mouse)
UseTagsBioID2-3xFLAGExpressionMammalianMutationPromoterCAG promoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-CD44b-TurboID
Plasmid#163850PurposeIn vitro transcription of CD44b-TurboID. Parton lab clone JTJDepositorInsertCD44b (cd44b Zebrafish)
UseIn vitro transcription of rnaTagsTurboIDExpressionMutationPromoterAvailable sinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-BASU-EGFP
Plasmid#163845PurposeIn vitro transcription of BASU-EGFP. Parton lab clone JRRDepositorInsertBASU
UseIn vitro transcription of rnaTagsEGFPExpressionMutationPromoterAvailable sinceSept. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTP264
Plasmid#149334PurposeBacterial expression of Biotin ligase BirADepositorInsertBiotin ligase BirA
UseTagsHis14 and bdNEDD8ExpressionBacterialMutationPromoterAvailable sinceJune 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET21d-myc-BirA
Plasmid#109424PurposeBacterial expression of biotin ligase (BirA) with an N-terminal myc epitopeDepositorInsertBirA (birA )
UseTagsmycExpressionBacterialMutationPromoterT7Available sinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCKmatBC
Plasmid#138587PurposematBC cassette from R. trifolii controlled by a Plac promoter; pSC101 ori, catDepositorInsertbicistronic cassette containing a malonate transporter (matC) and a malonyl-CoA synthetase (matB)
UseSynthetic BiologyTagsExpressionBacterialMutationmatB contains native GUG start codonPromoterPlac promoterAvailable sinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUASt-GBP-Trbo
Plasmid#179996PurposeExpresses two tandem copies of the GFP nanobody fused at the C-terminus with TurboID. Expression is driven using the UAS-Gal4 system. The UASt version is ideal for expression in somatic tissues.DepositorInsertGBP nanobody
UseTagsTrboExpressionInsectMutationPromoterAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBAD-BirA-eCPX-AP(K10)
Plasmid#121908PurposeExpresses BirA and eCPX-AP(K10)DepositorInsertsUseTags6xHisExpressionBacterialMutationchanged lysine 10 in the AP to alaninePromoterT7 and pBAD promoterAvailable sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only