Addgene

We narrowed to 183 results for: Vibrio

Showing: 81 - 100 of 183 results

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pSL2623 (pCas6_Vch)

Plasmid

#200895

Purpose

Expresses human codon-optimized V. cholerae Cas6 from a CMV promoter.

Depositor

Samuel H. Sternberg

Article

Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

Insert

VchCas6

Use

Tags

NLS

Expression

Mammalian

Mutation

Promoter

Available since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pSL2622 (pCas7_Vch)

Plasmid

#200894

Purpose

Expresses human codon-optimized V. cholerae Cas7 from a CMV promoter.

Depositor

Samuel H. Sternberg

Article

Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

Insert

VchCas7

Use

Tags

NLS

Expression

Mammalian

Mutation

Promoter

Available since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pTetQCas-BsaI

Plasmid

#170629

Purpose

Expression of CRISPR-associated transposases

Depositor

Sheng Yang

Article

Zhang et al CRISPR J. 2021 Jun;4(3):350-359. doi: 10

Insert

VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(BsaI)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pTetQCas-8+IS186

Plasmid

#170636

Purpose

Expression of CRISPR-associated transposases

Depositor

Sheng Yang

Article

Zhang et al CRISPR J. 2021 Jun;4(3):350-359. doi: 10

Insert

VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(8+IS186 array)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pSL0426 (CRISPR with spc3)

Plasmid

#130642

Purpose

Expresses CRISPR RNA from a T7 promoter for VchCAST system. The CRISPR array encodes a guide RNA with spacer 3 from the native CRISPR.

Depositor

Samuel H. Sternberg

Article

Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

Insert

CRISPR(spacer-3)

Use

CRISPR; Transposon

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pSL0373 (His-MBP-TniQ)

Plasmid

#130638

Purpose

Expresses V. cholerae CAST TniQ from a T7 promoter, with N-terminal His10-MBP-TEVsite tag for purification.

Depositor

Samuel H. Sternberg

Article

Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

Insert

VchTniQ

Use

CRISPR; Transposon

Tags

His10-MBP-TEVsite on TniQ

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pSL0721 (pDonor_L1-105)

Plasmid

#130645

Purpose

Encodes a mini-transposon derived from V. cholerae Tn6677 CAST, with CmR cargo gene and shortened left end. Total transposon size = 933 bp.

Depositor

Samuel H. Sternberg

Article

Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

Insert

VchCAST donor DNA (short L)

Use

CRISPR; Transposon

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pSL0715 (pDonor_R1-57)

Plasmid

#130644

Purpose

Encodes a mini-transposon derived from V. cholerae Tn6677 CAST, with CmR cargo gene and shortened right end. Total transposon size = 910 bp.

Depositor

Samuel H. Sternberg

Article

Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

Insert

VchCAST donor DNA (short R)

Use

CRISPR; Transposon

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pLPC31

Plasmid

#209996

Purpose

Contains Level 0 Part: 3' Connector (3C2SN) for the construction of Level 1 plasmids

Depositor

Thorsten Mascher, Georg Fritz

Article

Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

Insert

3' Connector (3C2SN)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pLPC32

Plasmid

#209997

Purpose

Contains Level 0 Part: 3' Connector (3C5CSN) for the construction of Level 1 plasmids

Depositor

Thorsten Mascher, Georg Fritz

Article

Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

Insert

3' Connector (3C5CSN)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pLP-sfgfp

Plasmid

#209994

Purpose

Contains Level 0 Part: Coding sequences (sfGFP) for the construction of Level 1 plasmids

Depositor

Thorsten Mascher, Georg Fritz

Article

Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

Insert

sfGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pLPT1

Plasmid

#209995

Purpose

Contains Level 0 Part: Terminator (TB1006) for the construction of Level 1 plasmids

Depositor

Thorsten Mascher, Georg Fritz

Article

Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

Insert

Terminator (TB1006)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pLPC52

Plasmid

#209991

Purpose

Contains Level 0 Part: 5' Connector (5C2SN) for the construction of Level 1 plasmids

Depositor

Thorsten Mascher, Georg Fritz

Article

Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

Insert

5' Connector (5C2SN)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pLPS1

Plasmid

#209992

Purpose

Contains Level 0 Part: Ribosome binding site (RB0034) for the construction of Level 1 plasmids

Depositor

Thorsten Mascher, Georg Fritz

Article

Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

Insert

RBS (RB0034)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pLPC51

Plasmid

#209990

Purpose

Contains Level 0 Part: 5' Connector (5C1CSN) for the construction of Level 1 plasmids

Depositor

Thorsten Mascher, Georg Fritz

Article

Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

Insert

5' Connector (5C1CSN)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pLP2

Plasmid

#209989

Purpose

Entry vector to store Level 0 resistance cassette parts and E. coli shuttle part (containing no E. coli ori)

Depositor

Thorsten Mascher, Georg Fritz

Article

Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pAraQCas-BsaI

Plasmid

#170633

Purpose

Expression of CRISPR-associated transposases

Depositor

Sheng Yang

Article

Zhang et al CRISPR J. 2021 Jun;4(3):350-359. doi: 10

Insert

VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(BsaI)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pLP-mScarlet-I

Plasmid

#209993

Purpose

Contains Level 0 Part: Coding sequences (mScarlet-I) for the construction of Level 1 plasmids

Depositor

Thorsten Mascher, Georg Fritz

Article

Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

Insert

mScarlet-I

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pST_116_LVL2 cam

Plasmid

#179332

Purpose

NT-CRISPR plasmid for a single gRNA.

Depositor

Anke Becker

Article

Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:

Insert

Ptac tfoX, Ptet cas9, PJ23106 acrIIA4, Ptet gRNA with sfGFP dropout

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

March 21, 2022

Availability

Academic Institutions and Nonprofits only

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pSL2783 (pTnsC-VP64_Vch)

Plasmid

#200896

Purpose

Expresses human codon-optimized V. cholerae TnsC-VP64 from a CMV promoter.

Depositor

Samuel H. Sternberg

Article

Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

Insert

VchTnsC-VP64

Use

Tags

NLS

Expression

Mammalian

Mutation

Promoter

Available since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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