Purpose
Expresses both fragments of T7 RNAP split at position 179. The N-terminal fragment is driven by PLac while the C-terminal fragment is driven by PBAD. C-terminal fragment has the point mutations N748D.
Inserts
Residues 1-179 of split T7 RNAP
Residues 180-880 of split T7 RNAP
Use
Synthetic Biology
Tags
Expression
Bacterial
Mutation
Residues 1-180 of split T7 RNAP and Residues 180-…
Available since
Nov. 5, 2014
Availability
Academic Institutions and Nonprofits only