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Plasmid#82023Purposean updated version of cLac143 (pACSA_PcptOO_C38T,A39T_YFP_PMB2_LacIWF). The homologous region upstream of the inducible system was fixed to add a stop codon for A1837. No difference in expressionDepositorInsertYFP
UseTagsExpressionMutationPromoterAvailable sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSW039
Plasmid#140035PurposeKO of PCC 11901 psbA2 gene/expresses YFP under control of inducible cLac143 promoterDepositorInsertYellow fluorescent protein
UseTagsExpressionBacterialMutationPromotercLac143Available sinceJan. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSW036
Plasmid#140034PurposeKO of PCC 11901 acsA gene/expresses YFP under control of the cpt promoterDepositorInsertYellow fluorescent protein
UseTagsExpressionBacterialMutationPromotercptAvailable sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET41-GST-KaiC
Plasmid#68051PurposeStrain: AM5117, Used for protein purificationDepositorInsertKaiC
UseTags6xHis, GST, and S-TagExpressionBacterialMutationPromoterT7Available sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28-SUMO-KaiB
Plasmid#68050PurposeStrain: AM5116, Used for protein purificationDepositorInsertKaiB
UseTags6xHis and SUMOExpressionBacterialMutationPromoterT7Available sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
rbcLS-pMAL-2px
Plasmid#41621DepositorInsertrbcL/rbcS
UseTagsExpressionBacterialMutationPromoterPtacAvailable sinceNov. 29, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCcmO-StrepII-PDT
Plasmid#165584PurposeNative CcmO fusion with a StrepII tag and PDTDepositorInsertCcmO-PDT
UseTagsProtein degradation tagExpressionBacterialMutationPromoterAvailable sinceNov. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28-SUMO-KaiA
Plasmid#68049PurposeStrain: AM5115, Used for protein purificationDepositorInsertKaiA
UseTags6xHis and SUMOExpressionBacterialMutationPromoterT7Available sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pALM174
Plasmid#82022Purposean updated version of cLac94 (pACSA_PcptOO_YFP_PMB2__LacIWF). The homologous region upstream of the inducible system was fixed to add a stop codon for A1837. No difference in expressionDepositorInsertYFP
UseTagsExpressionMutationPromoterPcptooAvailable sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pC0.423
Plasmid#203947PurposeLevel 0 part. 2,4-diacetylphloroglucinol (DAPG)-inducible phlF_PphlF promoter.DepositorInsertphlF-PphlF
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
CRISPR-psbA2 point mutation
Plasmid#182929Purposepoint mutation on psbA2 by CRISPR in Synechocystis 6803DepositorInsertsddcpf1
gRNA targeting psbA2 in Synechocystis 6803: gatcttcggtcgcttgatctttc
psbA
UseCRISPRTagsExpressionMutationS264A, and silent mutation to remove PAM sitePromoterAvailable sinceApril 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCas73
Plasmid#82398PurposeΔccm:Km, used to delete ccm operonDepositorInsertccm deletion
UseTagsExpressionMutationPromoternoneAvailable sinceSept. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRSET FLIPPi-200u
Plasmid#13555DepositorInsertFLIPPi G162A
UseTagsECFP and EYFPExpressionBacterialMutationG162A in binding proteinPromoterAvailable sinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pRSET FLIPPi-30m
Plasmid#13556DepositorInsertFLIPPi T22A
UseTagsECFP and EYFPExpressionBacterialMutationT22A in binding proteinPromoterAvailable sinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pRSET FLIPPi-5u
Plasmid#13554DepositorInsertFLIPPi S52A
UseTagsECFP and EYFPExpressionBacterialMutationS52A in binding proteinPromoterAvailable sinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pC1.530
Plasmid#205441PurposeLevel 1 Position 1 pUC19 vector with sgRNA and GmR. Used for homologous recombination with Cas12a editing vector pC1.509. sgRNA targets pC1.509 for removal.DepositorInsertRepB gRNA
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSW068
Plasmid#140036PurposeKO of PCC 11901 fadD gene/expresses codon-optimized 'tesA gene from E. coli from inducible cLac143 promoterDepositorInsertTruncated thioesterase gene
UseTagsExpressionBacterialMutationPromotercLac143Available sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRSET FLIPPi-260n
Plasmid#13552DepositorInsertFLIPPi WT
UseTagsECFP and EYFPExpressionBacterialMutationPromoterAvailable sinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pSZT025
Plasmid#140033PurposeKO of PCC 7002 fadD gene/expresses codon-optimized 'tesA gene from E. coli from inducible cLac143 promoterDepositorInsertTruncated thioesterase gene
UseTagsExpressionBacterialMutationPromotercLac143Available sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCas5
Plasmid#82378PurposesgRNA targeting YFP expressed from pJ23119 in the NS1 locus with kanamycin resistanceDepositorInsertgRNA
UseTagsExpressionMutationPromoterPJ23119Available sinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only