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Plasmid#105813PurposeFunctional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear mCherry expression marker; your protein of interest with C-terminal FLAG.DepositorTypeEmpty backboneUseRNAi; Flp-in competentTagsTEV-FLAGExpressionMammalianMutationPromoterAvailable sinceMarch 5, 2018AvailabilityAcademic Institutions and Nonprofits only
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pMNK55 - piRNAi 21ur-1224 - cku-80
Plasmid#159820PurposepiRNA-induced RNA interference (piRNAi) to bias double-strand repair toward homologous recombinationDepositorInsertcku-80 piRNAi 21ur-1224
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMNK54 - piRNAi 21ur-1224 - him-5
Plasmid#159818PurposepiRNA-induced RNA interference (piRNAi) to induce males in transgenic animalsDepositorInserthim-5 piRNAi 21ur-1224
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMNK56 - piRNAi 21ur-1224 - him-8
Plasmid#159819PurposepiRNA-induced RNA interference (piRNAi) to induce males in transgenic animalsDepositorInserthim-8 piRNAi 21ur-1224
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEN_TT 3XFLAG-wtGATA6-3XAU1_RNAi resistant
Plasmid#72922PurposeGateway entry vector for an inducible N-terminally 3XFLAG-tagged and C-terminally AU1-tagged, RNAi resistant human wtGATA6DepositorInsertGATA Binding Protein 6 (GATA6 Human)
UseEntry vector for gateway cloningTags3XAU1 and 3XFLAGExpressionBacterialMutationPromoterTRE tightAvailable sinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
3789 pmko-neo RNAi-GFP
Plasmid#8881DepositorInsertGFP
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pKK-RNAi-nucCHERRYmiR-EGFP-TEV
Plasmid#105810PurposeFunctional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear mCherry expression marker; your protein of interest with N-terminal EGFP.DepositorTypeEmpty backboneUseRNAi; Flp-in competentTagsEGFP-TEVExpressionMammalianMutationPromoterAvailable sinceMarch 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKK-RNAi-nucCHERRYmiR-TEV-EGFP
Plasmid#105814PurposeFunctional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear mCherry expression marker; your protein of interest with C-terminal EGFP.DepositorTypeEmpty backboneUseRNAi; Flp-in competentTagsTEV-EGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-mSNX27-H112A RNAi-resistant
Plasmid#163621Purposemouse SNX27 cDNA with point mutation to resist siRNA against SNX27 and with a point mutation H112A C-terminally tagged with GFPDepositorInsertmouse SNX27-H112A RNAi-resistant (Snx27 Mouse)
UseTagsGFPExpressionMammalianMutationH112A and RNAi resistancePromoterAvailable sinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKK-RNAi-nucCHERRYmiR-FLAG-TEV
Plasmid#105809PurposeFunctional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear mCherry expression marker; your protein of interest with N-terminal FLAG.DepositorTypeEmpty backboneUseRNAi; Flp-in competentTagsFLAG-TEVExpressionMammalianMutationPromoterAvailable sinceFeb. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-myc-Rem2 RNAiR2 short
Plasmid#51596Purposeexpresses RNAiR myc-tagged rat Rem2 (short version, missing 69 AA at N terminus)DepositorInsertRem2 (Rem2 Rat)
UseTagsmycExpressionMammalianMutationsilent mutations at shRNA 1 and 2 recognition sit…PromoterCMVAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
UB13p::dsSPL10 v01 (SPL10-RNAi)
Plasmid#27409DepositorInsertSPL10 inverted (AT1G27370 Mustard Weed)
UseTagsExpressionMutationPromoterAvailable sinceJan. 20, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-TO-HA-SH3BP1 (RNAi-res)
Plasmid#136645PurposeExpression of SH3BP1 in mammalian cells; resistant to siRNA targeting GAUGACAGCCACCCACUUCDepositorInsertSH3BP1 (RNAi resistant) (SH3BP1 Human)
UseTagsHA (MQDLPGNDNSTAG)ExpressionMammalianMutationGATGACAGCCACCCACTTC changed to AATGACAGCAACACACTTCPromoterAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR FL CPAP RNAi resistant
Plasmid#46390DepositorInsertCPAP RNAi resistant mutant (CENPJ Human)
UseEntry vectorTagsExpressionMutationsilent mutation that renders it resistant to siRN…PromoterAvailable sinceJuly 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLYS1-EMRE/RNAi resistant
Plasmid#50056PurposeExpression of human EMRE in mammalian cells with silent mutation making it resistant to RNAiDepositorInsertEMRE-RNAi resistant (SMDT1 Human)
UseTagsExpressionMammalianMutationRNAi resistant, silent mutantPromoterAvailable sinceFeb. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-myc-Rem2 RNAiR2
Plasmid#51585Purposeexpresses RNAi-resistant (RNAiR) myc-tagged mouse Rem2 (long version)DepositorInsertRem2 (Rem2 Mouse)
UseTagsmycExpressionMammalianMutationsilent mutations at shRNA 1 and 2 recognition sit…PromoterCMVAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pbs/pU6-HDAC4 RNAi
Plasmid#21100DepositorInsertRNAi against histone deacetylase4 (HDAC4 Mouse, Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-mSNX27 RNAi-resistant
Plasmid#163620Purposemouse SNX27 cDNA with point mutation to resist siRNA against SNX27 C-terminally tagged with GFPDepositorInsertmouse SNX27 RNAi-resistant (Snx27 Mouse)
UseTagsGFPExpressionMammalianMutationRNAi resistancePromoterAvailable sinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
Bnip3L RNAi pSuper-retro
Plasmid#17468DepositorInsertBnip3L RNAi (BNIP3L Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCMV-myc-Sema4D RNAiR
Plasmid#51600Purposeexpresses RNAi-resistant mouse Sema4D with N-terminal myc tagDepositorInsertSema4D (Sema4d Mouse)
UseTagsmycExpressionMammalianMutationsilent mutations between amino acids (approx 275-…PromoterCMVAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only