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pEMB52 LRRK2_BC_1520_2148_I2149ENLYFQGWSHPQFEK

Plasmid

#40566

Depositor

Michael J Fox Foundation MJFF

Insert

LRRK2 (LRRK2 Human)

Use

Baculovirus

Tags

6xHis and Tev-Strep (ENLYFQGWSHPQFEK)

Expression

Mutation

WT; aa1520_2148

Promoter

polH

Available since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

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pEMB018 LRRK2_BC_1520_2148_K1906M

Plasmid

#40567

Depositor

Michael J Fox Foundation MJFF

Insert

LRRK2 (LRRK2 Human)

Use

Baculovirus

Tags

6xHis

Expression

Mutation

aa1520_2148; K1906M

Promoter

polH

Available since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

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pEMB017 NP_9409803_LRRK2_1867_2161_L1914N_V2048T_F2083Y

Plasmid

#40427

Depositor

Michael J Fox Foundation MJFF

Insert

LRRK2 (LRRK2 Human)

Use

Baculovirus

Tags

GST-Tev and HisTev

Expression

Mutation

aa1867_2161; L1914N, V2048T, F2083Y

Promoter

polH

Available since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

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pEMB018 / LRRK2_BC_1300_2148

Plasmid

#40500

Depositor

Michael J Fox Foundation MJFF

Insert

LRRK2 (LRRK2 Human)

Use

Baculovirus

Tags

6xHis

Expression

Mutation

WT; aa1300_2148

Promoter

polH

Available since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

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pEMB018 / LRRK2_BC_1300_2170

Plasmid

#40501

Depositor

Michael J Fox Foundation MJFF

Insert

LRRK2 (LRRK2 Human)

Use

Baculovirus

Tags

6xHis

Expression

Mutation

WT; aa300_2170

Promoter

polH

Available since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

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pEMB018 / LRRK2_BC_1300_2210

Plasmid

#40502

Depositor

Michael J Fox Foundation MJFF

Insert

LRRK2 (LRRK2 Human)

Use

Baculovirus

Tags

6xHis

Expression

Mutation

WT; aa1300_2210

Promoter

polH

Available since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

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pCS-memb-mCherry

Plasmid

#53750

Purpose

Contains mCherry fused to a 2x membrane localization sequence in the pCS2+ vector.

Depositor

Sean Megason

Article

Megason et al Methods Mol Biol. 2009;546:317-32. doi:

Insert

2x membrane linker

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

simian CMV IE94

Available since

June 10, 2014

Availability

Academic Institutions and Nonprofits only

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pCS-membrane-ceruleanFP

Plasmid

#53749

Purpose

Contains membrane localized cerulean fluorescent protein in pCS expression vector.

Depositor

Sean Megason

Article

Megason et al Methods Mol Biol. 2009;546:317-32. doi:

Insert

2x membrane linker

Use

Tags

Cerulean

Expression

Mammalian

Mutation

Promoter

simian CMV IE94

Available since

June 10, 2014

Availability

Academic Institutions and Nonprofits only

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pQC membrane TdTomato IX

Plasmid

#37351

Depositor

Connie Cepko

Article

Beier et al Dev Biol. 2011 May 15;353(2):309-20. Epu

Insert

membrane TdTomato

Use

Tags

Palmitoylation signal*

Expression

Mutation

Promoter

Available since

July 30, 2012

Availability

Academic Institutions and Nonprofits only

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pGEMHE-membrane-mEGFP

Plasmid

#105526

Purpose

T7 promotor drives in vitro mRNA transcription of a mEGFP-tagged membrane reporter

Depositor

Melina Schuh

Article

Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

Insert

GNAI2 (GNAI2 Human)

Use

In vitro transcription

Tags

mEGFP

Expression

Mutation

fragment encoding the plasma membrane targeting s…

Promoter

T7

Available since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

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pSP64-membrane-mCherry

Plasmid

#135598

Purpose

Expression of membrane-mCherry mRNA for microinjection for visualization of membrane in S. pupuratus

Depositor

Mamiko Yajima

Article

Uchida et al Dev Biol. 2018 Sep 1;441(1):19-30. doi:

Insert

membrane-mCherry

Use

In vitro transcription (mrna synthesis)

Tags

mCherry

Expression

Mutation

Promoter

Unknown

Available since

Jan. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pQC membrane Venus IX

Plasmid

#37343

Depositor

Connie Cepko

Article

Beier et al Dev Biol. 2011 May 15;353(2):309-20. Epu

Insert

membrane Venus

Use

Tags

Expression

Mutation

Promoter

Available since

July 27, 2012

Availability

Academic Institutions and Nonprofits only

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pQC membrane CFP IX

Plasmid

#37336

Depositor

Connie Cepko

Article

Beier et al Dev Biol. 2011 May 15;353(2):309-20. Epu

Insert

membrane CFP

Use

Tags

Expression

Mutation

Promoter

Available since

July 27, 2012

Availability

Academic Institutions and Nonprofits only

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pCLASP_Crz1(19A)_CLASP_RGS2membrane

Plasmid

#133085

Purpose

Plasmid contains Crz1* with CLASP. This consists of the plasma membrane anchor (pm-LOVTRAP) and the cargo (Zdk-Crz1*-yeLANS). CLASP modulates nuclear localization of Crz1* in response to blue light.

Depositor

Hana El-Samad

Article

Chen et al Cell Syst. 2020 Sep 4. pii: S2405-4712(2

Insert

Crz1*-CLASP

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

pADH1

Available since

Sept. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pQC membrane mKorange IX

Plasmid

#37345

Depositor

Connie Cepko

Article

Beier et al Dev Biol. 2011 May 15;353(2):309-20. Epu

Insert

membrane mKusabira Orange

Use

Tags

Expression

Mutation

Promoter

Available since

July 27, 2012

Availability

Academic Institutions and Nonprofits only

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pEMB36(revcomp)/Dvl1_1_670

Plasmid

#40557

Depositor

Michael J Fox Foundation MJFF

Insert

DVL1 (DVL1 Human)

Use

Baculovirus

Tags

Expression

Mutation

WT; aa1_670

Promoter

polH

Available since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

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pEntry_HCoV-229E-Membrane

Plasmid

#168966

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

HCoV-229E-Membrane (M )

Use

Gateway-compatible entry vector

Tags

Expression

Mutation

Promoter

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pEntry_HCoV-NL63-Membrane

Plasmid

#168925

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

HCoV-NL63-Membrane (HCNV63gp5 )

Use

Gateway-compatible entry vector

Tags

Expression

Mutation

Promoter

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pEntry_HCoV-OC43-Membrane

Plasmid

#168946

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

HCoV-OC43-Membrane (M )

Use

Gateway-compatible entry vector

Tags

Expression

Mutation

Promoter

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pEntry_HCoV-HKU1-Membrane

Plasmid

#168905

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

HCoV-HKU1-Membrane (M )

Use

Gateway-compatible entry vector

Tags

Expression

Mutation

Promoter

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge