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Showing: 81 - 100 of 144 results

  2. pAH-CTX1-rhadCas9-native

    Plasmid
    #129392
    Purpose
    Derived from pAH-CTX1-rha. The native Streptococcus pyogenes dCas9 gene cloned downstream of PrhaBAD. Non-codon-optimized dCas9 version of pAH-CTX1-rhadCas9.
    Depositor
    Silvia Cardona
    Article
    Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021
    Insert
    S. pyogenes dCas9
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Aspartate 10 to Alanine, Histidine 840 to Alanine
    Promoter
    PrhaBAD
    Available since
    Dec. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    129392-plasmid-map-sequence-id-266824

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  6. pAH18

    Plasmid
    #154397
    Purpose
    Derived from pGPI-SceI; Trimethoprim resistant; carries I-SceI gene; contains 475 bp from both upstream and downstream flanking regions between the FRT sites on pAHCTX1-rhadCas9
    Depositor
    Silvia Cardona
    Article
    Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021
    Insert
    Fused upstream and downstream homologous regions around FRT sites when pAH-CTX1-rhadCas9 integrated into K56-2 genome
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    NA
    Available since
    Aug. 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    154397-plasmid-map-sequence-id-308811

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  10. pBBR1-Rha-ETh_tji49-kan

    Plasmid
    #166669
    Purpose
    Genome engineering of Burkholderia
    Depositor
    Jun Fu
    Insert
    ETh_tji4
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    166669-plasmid-map-sequence-id-329553

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  11. pBBR1-Rha-ETh1h2e_yi23-kan

    Plasmid
    #166670
    Purpose
    Genome engineering of Burkholderia
    Depositor
    Jun Fu
    Insert
    ETh1h2e_yi23
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    166670-plasmid-map-sequence-id-329556

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  17. pSCrhaB2_ApyD_ RBS_ApyAOHIS

    Plasmid
    #226247
    Purpose
    Production of ApyA peptide modified by the B12-rSAM enzyme ApyD (optional, refer to publication), the cytochrome P450 enzyme ApyO, and the MNIO enzyme ApyHI, and the methyltransferase ApyS
    Depositor
    Wilfred van der Donk
    Article
    Nguyen et al ACS Cent Sci. 2024 Apr 11;10(5):1022-103
    Inserts
    His6_ApyA
    ApyD
    ApyO
    ApyH
    ApyI
    ApyS
    Use
    Tags
    Hexa-Histidine
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    226247-plasmid-map-sequence-id-450057

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  18. pSCrhaB2_ApyOAHIDS

    Plasmid
    #226246
    Purpose
    Production of ApyA peptide modified by the B12-rSAM enzyme ApyD (optional, refer to publication), the cytochrome P450 enzyme ApyO, and the MNIO enzyme ApyHI, and the methyltransferase ApyS
    Depositor
    Wilfred van der Donk
    Article
    Nguyen et al ACS Cent Sci. 2024 Apr 11;10(5):1022-103
    Inserts
    His6_ApyA
    ApyD
    ApyO
    ApyH
    ApyI
    ApyS
    Use
    Tags
    Hexa-Histidine
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    226246-plasmid-map-sequence-id-450056

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Showing: 81 - 100 of 144 results