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Plasmid Kit#1000000224PurposeThis kit consists of ten ONE vector G protein Optical (ONE-GO) biosensor plasmids for the expression of BRET-based biosensors of G proteins activated by G-protein-coupled receptors (GPCRs).DepositorAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only
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pLenti_CMV:Flag-GpNLuc
Plasmid#208840PurposeExpresses FLAG epitope-tagged GpNLuc "Green" BRET reporter in mammalian cells.DepositorInsertGpNLuc LumiFluor
UseLentiviralTags1xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CMV:Flag-Antares
Plasmid#208852PurposeExpresses FLAG epitope-tagged Antares "Orange" BRET reporter in mammalian cells.DepositorInsertAntares
UseLentiviralTags1xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CMV:Flag-YeNL
Plasmid#208849PurposeExpresses FLAG epitope-tagged YeNL "Yellow" BRET reporter in mammalian cells.DepositorInsertYeNL Nanolantern
UseLentiviralTags1xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CMV:Flag-OeNL
Plasmid#208850PurposeExpresses FLAG epitope-tagged OeNL "Orange" BRET reporter in mammalian cells.DepositorInsertOeNL Nanolantern
UseLentiviralTags1xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CMV:Flag-OgNLuc
Plasmid#208851PurposeExpresses FLAG epitope-tagged OgNLuc "Orange" BRET reporter in mammalian cells.DepositorInsertOgNLuc LumiFluor
UseLentiviralTags1xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGDP3 cat
Plasmid#112888PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.DepositorInsertcat (Brevibacillus brevis) (cat Brevibacillus brevis)
UseTags6xHisExpressionBacterialMutationPromoterPblaAvailable sinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CMV:Flag-KaNLuc
Plasmid#208855PurposeExpresses FLAG epitope-tagged KaNLuc "Red" BRET reporter in mammalian cells.DepositorInsertKaNLuc LumiFluor
UseLentiviralTags1xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CMV:Flag-Lumiscarlet
Plasmid#208854PurposeExpresses FLAG epitope-tagged Lumiscarlet "Red" BRET reporter in mammalian cells.DepositorInsertLumiscarlet
UseLentiviralTags1xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CMV:Flag-dKeNLuc
Plasmid#208856PurposeExpresses FLAG epitope-tagged dKeNLuc "Red" BRET reporter in mammalian cells.DepositorInsertdKeNLuc LumiFluor
UseLentiviralTags1xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CMV:Flag-CeNLuc
Plasmid#208838PurposeExpresses FLAG epitope-tagged CeNLuc "Blue" BRET reporter in mammalian cells.DepositorInsertCeNLuc LumiFluor
UseLentiviralTags1xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CMV:Flag-CeNL
Plasmid#208839PurposeExpresses FLAG epitope-tagged CeNL "Blue" BRET reporter in mammalian cells.DepositorInsertCeNL Nanolantern
UseLentiviralTags1xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CMV:Flag-GeNL
Plasmid#208841PurposeExpresses FLAG epitope-tagged GeNL "Green" BRET reporter in mammalian cells.DepositorInsertGeNL Nanolantern
UseLentiviralTags1xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL3-sbIL6P
Plasmid#65099PurposeLuciferase reporter construct under the seabream IL6 promoter.DepositorInsertinterleukin-6 promoter
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceJan. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-rB50
Plasmid#18957DepositorInsertBrevican N-terminus (Bcan Rat)
UseTagsExpressionMammalianMutationBrevican N-terminus, aminoacids 1-395PromoterAvailable sinceDec. 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
FgH1tUTG_mup53_Ex4
Plasmid#85534PurposeInducible expression of guide RNA (mup53_Ex4) with fluorescent GFP reporterDepositorInsertmu p53
UseTagsExpressionMutationPromoterH1tAvailable sinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
FgH1tUTG_muBim_Ex3
Plasmid#85533PurposeInducible expression of guide RNA (muBim_Ex3) with fluorescent GFP reporterDepositorInsertmu Bim
UseTagsExpressionMutationPromoterH1tAvailable sinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
FgH1tUTG
Plasmid#70183PurposeInducible expression of guide RNA with fluorescent GFP reporterDepositorInsertH1-Tet-sgrna cassette
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
FUCas9Cherry
Plasmid#70182PurposeExpresses Cas9 with fluorescent Cherry reporterDepositorInserthumanised S.pyogenes cas9
UseCRISPR and LentiviralTags3xFLAGExpressionMammalianMutationPromoterhUbCAvailable sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
FgH1tUTG_huBim_Ex3
Plasmid#85532PurposeInducible expression of guide RNA (huBim_Ex3) with fluorescent GFP reporterDepositorInserthu Bim
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterH1tAvailable sinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only