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Plasmid#91786PurposeConstitutive expression of mTagBFP2-Dectin1A-N-10 using PGK promoter in a lentiviral backbone.DepositorInsertDectin1A (CLEC7A Human)
UseLentiviralTagsmTagBFP2ExpressionMammalianMutationPromoterPGKAvailable sinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
1x_a1gRNAOp_pCYC1m_yeBFP2
Plasmid#64390Purposeencodes one a1 operator site upstream of minimal pCYC1 promoter driving yeast enhanced BFPDepositorInsertyeast enhanced BFP2
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
oxBFP
Plasmid#68063PurposeC-terminal fusion to oxBFPDepositorTypeEmpty backboneUseTagsoxBFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pExp_TRE:EBFP2
Plasmid#51793PurposeInducible expression of EBFP2 with rtTA for input fluorescenceDepositorInsertEBFP2
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterTREAvailable sinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
MXS_TagBFP
Plasmid#62400PurposeMXS_chaining vector with TagBFPDepositorInsertTagBFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLEX_970_puro_DEST_V5_BFP
Plasmid#45297DepositorInsertBFP
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceMay 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_G4_Q118R_EBFP_to_EGFP_Dual_pegRNA
Plasmid#178100PurposeControl vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 Q118R-G4 and EBFP_To_EGFP pegRNAs from two independent U6 promoters.DepositorInsertATP1A1 G4 Q118R pegRNA + EBFP to EGFP pegRNA
UseCRISPR; Prime editingTagsExpressionMammalianMutationPromoterTandem U6 promotersAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
NAX_CAGGS_CAS9_m4_VPR_EBFP2
Plasmid#167881PurposePiggyBac compatible plasmid expressing dCas9-VPRDepositorInsertdCas9-VPR
UseCRISPRTagsExpressionMutationD10A, D839A, H840A, N863APromoterAvailable sinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
cfSBFP2-N
Plasmid#110633PurposeCys-free blue fluorescent protein for extracellular imaging. SBFP2 variant (C48S/C70M). Molecular brightness is almost comparable to SBFP2.DepositorInsertcfSBFP2
UseTagsExpressionMutationCys48Ser/Cys70MetPromoterAvailable sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
NAX_CAGGS_CAS9_EBFP2
Plasmid#167857PurposePiggyBac compatible plasmid expressing spCas9DepositorInsertCas9
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQLinkHD_EBFP2
Plasmid#118853Purposefluorescent protein expression with an N-terminal His-tagDepositorInsertEBFP2
UseTagsHis-tagExpressionBacterialMutationEBFP1.2-I128V/V150I/ D155V/V224RPromoterAvailable sinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCVL.SFFV.Kozak.HA.NLS.Y2Ani.IRES.BFP
Plasmid#45578PurposeExpresses Y2 variant of I-Ani I nuclease in mammalian cells with BFP trackerDepositorInsertcoY2 Ani
UseLentiviralTagsHA, IRES BFP, and NLSExpressionMammalianMutationco indicates codon optimization for mammalian exp…PromoterSFFVAvailable sinceNov. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
LCV2_BFP2
Plasmid#155097PurposeLentiviral backbone for cloning and expression of U6 driven gRNAs with Esp3I/BsmBI cloning sites, puromycin selection and BFP2 expressionDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcryBFP_Umyo18bGFP
Plasmid#172527PurposealphaA-crystallin promoter (cry) drives GFP expression in the lens. The 600bp unc-45b muscle-specific promoter drives expression of GFP-tagged zebrafish Myo18b.DepositorInsertmyo18b
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
NAX_CAGGS_CAS9_m4_KRAB_EBFP2
Plasmid#167873PurposePiggyBac compatible plasmid expressing dCas9-KRABDepositorInsertdCas9-KRAB
UseCRISPRTagsExpressionMutationD10A, D839A, H840A, N863APromoterAvailable sinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
12x_a1gRNAOp_pCYC1m_yeBFP2
Plasmid#64393Purposeencodes twelve a1 operator sites upstream of minimal pCYC1 promoter driving yeast enhanced BFPDepositorInsertyeast enhanced BFP2
UseTagsExpressionYeastMutationPromoterpCYC1Available sinceMay 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
6x_a1gRNAOp_pCYC1m_yeBFP2
Plasmid#64392Purposeencodes six a1 operator sites upstream of minimal pCYC1 promoter driving yeast enhanced BFPDepositorInsertyeast enhanced BFP2
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
psecBFP2
Plasmid#62230Purposeencodes an inert blue fluorescent protein for use in oxidizing cellular environments including the periplasm and mammalian secretory pathway.DepositorInsertsecBFP2
UseTagssecbfp2ExpressionMammalianMutationPromotercmvAvailable sinceJuly 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
3x_a1gRNAOp_pCYC1m_yeBFP2
Plasmid#64391Purposeencodes three a1 operator sites upstream of minimal pCYC1 promoter driving yeast enhanced BFPDepositorInsertyeast enhanced BFP2
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pWzl_neo_DEST_flag_BFP
Plasmid#45302DepositorInsertBFP
UseRetroviralTagsflagExpressionMammalianMutationPromoterAvailable sinceJune 27, 2013AvailabilityAcademic Institutions and Nonprofits only