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Plasmid#53467DepositorInsertRab05 (Rab5 Fly)
UseTagsdsRedExpressionInsectMutationQ88LPromoterGAL4Available sinceJune 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HA-mIFITM3-K83,88,104A
Plasmid#58405PurposeExpresses murine IFITM3-K83,88,104A with an N-terminal HA tag in mammalian cellsDepositorInsertIFITM3 (Ifitm3 Mouse)
UseTagsHAExpressionMammalianMutationLysines 83, 88, and 104 to AlaninePromoterCMV IEAvailable sinceSept. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
mOrange2-Vimentin-7
Plasmid#57977PurposeLocalization: Intermediate Filaments, Excitation: 549, Emission: 565DepositorInsertVimentin (VIM Human)
UseTagsmOrange2ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
5xtetO (tet3) Gene Desert
Plasmid#131339PurposegRNA to insert 5xTet operator sequence into a gene desert on chromosome 5.DepositorInsertDesert-gRNA
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUASP dsRed Rab5 DN
Plasmid#44823DepositorInsertRab5 (Rab5 Fly)
UseDrosophilaTagsdsRedExpressionInsectMutationS43N (dominant negative)PromoterAvailable sinceApril 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSUPERpuro-BglII-Hs4E-T_AC
Plasmid#148445PurposeMammalian Expression of Hs4E-T-shRNADepositorInsertHs4E-T-shRNA (EIF4ENIF1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pHR LAT-FFF/Zap70 iLID-Drop
Plasmid#171032PurposeLight-induced LAT-Zap70 dimerization and LAT clustering. Zap70 is TagRFP-tagged. LAT has three Tyr-Phe mutations.DepositorInsertLAT (LAT Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLpC-Zipp.-L-IFP-F[1]+Plum (mammalian)
Plasmid#52907PurposeBinary retroviral pl. (puromycin). Zipper (PacI/ClaI):Linker (GGGGS)X2+IFP-F[1]. In the second MCS, the Plum fluorescent protein is cloned in between PmeI/SalI. Confers ampicillin resistance to DH5α.DepositorInsertZipp.-L-IFP-F[1]+Plum
UseRetroviralTagsL-IFP-F[1]ExpressionMammalianMutationPromoterUnknownAvailable sinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEG302_dCAS9-MQ1(C141S,S317A)_g4+g10+g18
Plasmid#172317PurposeCRISPR dCas9 directly fused to a catalytic inactive version of bacterial DNA methyltransferase MQ1 to target to the FWA locus with three guide RNAsDepositorInsertg18_U6_g10_U6_g4_U6_UBQ10_Ω_dCas9_MQ1(C141S,S317A)_OCS
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xhyb PylT A41AA C55A LaG17-SynNotch 204TAG 442TAA
Plasmid#154779Purposeplasmid with 4xhybrid PylT cassette (mutant A41AA C55A) and LaG17-SynNotch 204TAG 277TAA, for transient transfection or stable, piggybac-mediated, integrationDepositorInsertLaG17-SynNotch
UseTagsLaG17ExpressionMammalianMutation204TAG 442TAA in LaG17-SynNotch, hybrid PylT with…PromoterEF1Available sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/AUG-stop-nLuc-3XFLAG
Plasmid#127308PurposeExpress 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon, but translation terminated immediately by stop codonDepositorInsertnanoLuciferase
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/CUG-nLuc-3XFLAG-CL1/PEST
Plasmid#127316PurposeExpress 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from CUG start codonDepositorInsertnanoLuciferase
UseTags3xFLAG and CL1/PESTExpressionMammalianMutationPromoterCMVAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJY-RpABE-FT_INF
Plasmid#112874Purposebinary vector for INF (Induced Neo-Functionalization) of FT in ArabidopsisDepositorInsertsRPS5A promoter
ABE7.10
U6-sgRNA targeting FT
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceSept. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJY-RpABE-PDS3_IMS
Plasmid#112875Purposebinary vector for IMS (Induced Mis-Splicing) of PDS3 in ArabidopsisDepositorInsertsRPS5A promoter
ABE7.10
U6-sgRNA targeting PDS3
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceSept. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pX330_CALR sgRNA / hSpCas9
Plasmid#172838PurposeMammalian expression of a sgRNA targeting the intron 1 of CALR (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting intron 1 of CALR under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB106-OEP7(1–50)
Plasmid#100602PurposeBinary vector for expression of Citrine-fused AtOEP7(1-50) (OEP7(1–50)-Citrine) to visualize chloroplast outer envelope membrane in plantsDepositorInsertOEP7(1–50)-Citrine (OEP7 Mustard Weed)
UseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/ACG-nLuc-3XFLAG-CL1/PEST
Plasmid#127318PurposeExpress 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from ACG start codonDepositorInsertnanoLuciferase
UseTags3xFLAG and CL1/PESTExpressionMammalianMutationPromoterCMVAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
Brm BDmut-Flag pShuttle
Plasmid#122108PurposeExpression of full length Brm containing point mutations in its bromodomain in mammalian cellsDepositorInsertBrm (SMARCA2 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMT3-RhoGC
Plasmid#100031PurposeThis plasmid is for expression of a rhodopsin/guanylyl cyclase fusion protein.DepositorInsertRhoGC
UseTags1D4 T9'A (AETSQVAPA) and C8 (PRGPDRPEGIEE)ExpressionMammalianMutationPromoterAdenovirus major late promoterAvailable sinceSept. 5, 2017AvailabilityAcademic Institutions and Nonprofits only