Addgene

We narrowed to 11,165 results for: cel.2

Showing: 9881 - 9900 of 11165 results

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pEF_PE2

Plasmid

#199267

Purpose

Mammalian expression of SpCas9 prime editor 2 under EF1α promoter

Depositor

Ting Zhou

Article

Li et al Nat Commun. 2022 Oct 27;13(1):6354. doi:

Insert

PE2-P2A-mp53DD

Use

Tags

Expression

Mammalian

Mutation

Δ40-903

Promoter

Available since

April 13, 2023

Availability

Academic Institutions and Nonprofits only

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tetO-FOSL1

Plasmid

#170687

Purpose

doxycycline-inducible overexpression of FOSL1

Depositor

Paul Burridge

Article

Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

Insert

FOSL1 (FOSL1 Human)

Use

Lentiviral; Doxycycline inducible

Tags

Expression

Mammalian

Mutation

Promoter

TRE promoter, Tet-ON

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3 HA eIF4GI (1-1599)

Plasmid

#45640

Depositor

Nahum Sonenberg

Article

Yanagiya et al Mol Cell Biol. 2009 Mar;29(6):1661-9. do

Insert

eIF4GI transcript variant 2 (EIF4G1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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HA-HIF2alpha-P405A/P531A-pcDNA3

Plasmid

#18956

Depositor

William Kaelin

Article

Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

Insert

Hypoxia inducible factor 2 alpha (EPAS1 Human)

Use

Tags

HA-tag

Expression

Mammalian

Mutation

cDNA changed amino acids: Proline 405 to Alanin…

Promoter

Available since

Aug. 20, 2008

Availability

Academic Institutions and Nonprofits only

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miniSOG-Mito-7

Plasmid

#57773

Purpose

Localization: Mitochondria, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Mito (COX8A Human)

Use

Tags

miniSOG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 29, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pGL3-B-161;+80pCalb2

Plasmid

#66745

Purpose

luciferase reporter for Calb2 promoter (-161to +80, WT)

Depositor

Emanuela Felley-Bosco, Rolf Stahel

Article

Kresoja-Rakic et al Oncotarget. 2016 Feb 1. doi: 10.18632/on

Insert

Calb2 promoter (-161bp+80) (CALB2 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

Calb2

Available since

Jan. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pGL3-B-264;+80pCalb2

Plasmid

#66746

Purpose

luciferase reporter for Calb2 promoter (-264 to +80, WT)

Depositor

Emanuela Felley-Bosco, Rolf Stahel

Article

Kresoja-Rakic et al Oncotarget. 2016 Feb 1. doi: 10.18632/on

Insert

Calb2 promoter (-264bp+80) (CALB2 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

Calb2

Available since

Jan. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pGL3-B-419;+80pCalb2

Plasmid

#66747

Purpose

luciferase reporter for Calb2 promoter (-419 to +80, WT)

Depositor

Emanuela Felley-Bosco, Rolf Stahel

Article

Kresoja-Rakic et al Oncotarget. 2016 Feb 1. doi: 10.18632/on

Insert

Calb2 promoter (-419bp+80) (CALB2 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

Calb2

Available since

Jan. 19, 2016

Availability

Academic Institutions and Nonprofits only

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(114) pcDNA3.1-GFP-Flag-JunB-EPEA

Plasmid

#160743

Purpose

Expresses a GFP tagged JunB with Flag and EPEA tags.

Depositor

Christina Woo

Article

Ramirez et al ACS Chem Biol. 2020 Apr 17;15(4):1059-10

Insert

GFP-Flag-JunB-EPEA (JUN Human)

Use

Tags

EPEA, Flag, and GFP

Expression

Mammalian

Mutation

Promoter

T7/CMV

Available since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

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mEmerald-Mito-7

Plasmid

#54160

Purpose

Localization: Mitochondria, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Planchon et al Nat Methods. 2011 May;8(5):417-23. doi:

Insert

Mito (COX8A Human)

Use

Tags

mEmerald

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 16, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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PB-PE

Plasmid

#162796

Purpose

All-in-one prime editor piggyBac transposon

Depositor

Tobias Cantz

Article

Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

Insert

SpCas9_ H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)

Use

CRISPR and Synthetic Biology; Piggybac transposon

Tags

SV40 NLS

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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Str-STIM1-NN_neomycin

Plasmid

#65311

Purpose

Luminal ER hook only (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

STIM1 mutated for binding motif to microtubule (STIM1 Human)

Use

Tags

streptavidin

Expression

Mammalian

Mutation

2 amino acids of STIM1 (IP from SxIP motif) were …

Promoter

CMV

Available since

June 10, 2015

Availability

Academic Institutions and Nonprofits only

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pAC94-pmax-dCas9VP160-2A-puro

Plasmid

#48226

Purpose

dCas9VP160-2A-puro (puro-selectable) on pmax expression vecor. Note: This is being tested.

Depositor

Rudolf Jaenisch

Article

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Insert

dCas9(D10A;H840A) fusion with VP160 activation domain followed by 2A-puro

Use

CRISPR

Tags

2A-puro, HA Tag, and VP160

Expression

Mammalian

Mutation

D10A;H840A (catalytically inactive)

Promoter

CAGGS

Available since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pMTS_mScarlet_N1

Plasmid

#85057

Purpose

In vivo visualization of the mitochondria (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

MTS (COX8A Human)

Use

Tags

mScarlet

Expression

Mammalian

Mutation

aa1-29

Promoter

CMV

Available since

March 16, 2017

Availability

Academic Institutions and Nonprofits only

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pg-proPHD2-PHD2-EGFP

Plasmid

#87210

Purpose

PHD2-EGFP expressed under the control of the PHD2 promoter

Depositor

Violaine Sée

Article

Bagnall et al J Biol Chem. 2014 Feb 28;289(9):5549-64.

Insert

PHD2 (EGLN1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

H313N

Promoter

PHD2

Available since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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ATG-2460

Plasmid

#108713

Purpose

EF1-CBR2opt-T2A-copGFP (construct used for lentiviral transduction)

Depositor

Keith Wood

Article

Hall et al Nat Commun. 2018 Jan 9;9(1):132. doi: 10

Insert

CBR2opt-copGFP fusion

Use

Lentiviral

Tags

CBR2opt fused to copGFP

Expression

Mutation

Promega used directed evolution to create CBR (Cl…

Promoter

elongation factor-1

Available since

Oct. 9, 2018

Availability

Academic Institutions and Nonprofits only

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HiBiT-FZD2

Plasmid

#195840

Purpose

Expresses human FZD2 with an N-terminal HiBiT

Depositor

Gunnar Schulte

Article

Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

Insert

FZD2 (FZD2 Human)

Use

Tags

HiBiT

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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MAC_N_LMNA

Plasmid

#111688

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

Insert

LMNA (LMNA Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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GFP-AuroraB kd K106R

Plasmid

#108493

Purpose

expression of EGFP-AuroraB kinase dead (K106R)

Depositor

Susanne Lens

Article

Hengeveld et al Mol Cell Proteomics. 2012 May;11(5):47-5

Insert

AuroraB (AURKB Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Kinase death K106A

Promoter

CMV

Available since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pUltraI-Tet3.0[TAA]

Plasmid

#164580

Purpose

Machinery plasmid containing a single copy of the Mb Tet3.0RS and cognate tRNA for incorporation of Tet3.0 at TAA codons

Depositor

Ryan Mehl

Article

Bednar et al ACS Chem Biol. 2021 Nov 19;16(11):2612-2

Inserts

Mb Tet3.0RS (R2-3)
Mm tRNA[TAA]

Use

Tags

Expression

Bacterial

Mutation

L170G, N311G, C313A

Promoter

ProK and TacI

Available since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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