Addgene

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pcDNA 3.1 - circMAN2A1

Plasmid

#198837

Purpose

This contains the 400nt isoform of exons two and three from MAN2A1 circular RNA, 3X Flag tag inserted in exon two

Depositor

Stefan Stamm

Article

Arizaca Maquera et al Front Mol Neurosci. 2023 May 5;16:114107

Insert

circular mannosidase alpha class 2A member 1

Use

Tags

Expression

Mammalian

Mutation

circular RNA composed by 2 exons

Promoter

CMV

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pET-Duet-6xHis-TEV-Nsp8(76-198)

Plasmid

#201024

Purpose

Bacterial expression of codon optimized SARS-CoV-2 nsp8 residues 76-198, with an N-terminus His tag followed by a TEV cleavage site.

Depositor

Sharon Rozovsky

Article

Ghelichkhani et al J Mol Biol. 2023 Apr 15;435(8):168008. d

Insert

non-structural protein 8 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)

Use

Tags

6xHis-TEV

Expression

Bacterial

Mutation

Gene insert is codon optimized for Ecoli.

Promoter

T7/LacO

Available since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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pLX317-VRK2

Plasmid

#199641

Purpose

Expresses VRK2

Depositor

William Hahn

Article

So et al JCI Insight. 2022 Oct 10;7(19):e158755.

Insert

VRK2 (VRK2 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pBMN HA-ATG13(Δ1-198)

Plasmid

#186222

Purpose

For expression of ATG13(Δ1-198) tagged with HA

Depositor

Michael Lazarou

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Insert

ATG13 (ATG13 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Deleted amino acids 1 - 198

Promoter

Available since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pCAGG-GST-TEV-NAP1 (WT)

Plasmid

#198034

Purpose

Used for the expression and purification of NAP1 in a mammalian expression system (SMC Internal No. 2064)

Depositor

Sascha Martens

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Insert

NAP1 (AZI2 Human)

Use

Tags

GST-TEV

Expression

Mammalian

Mutation

Promoter

Available since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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pFastBac_Dual_GST-TEV-EGFP-TBK1ΔCTD (res 1-666)

Plasmid

#198072

Purpose

Used for the expression and purification of EGFP labelled TBK1 carrying a mutation for the removal of the CTD (SMC Internal No. 1874)

Depositor

Sascha Martens

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Insert

TBK1 (TBK1 Human)

Use

Tags

GST-TEV-EGFP

Expression

Insect

Mutation

TBK1ΔCTD (res 1-666)

Promoter

Available since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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pFastBac_Dual_GST-TEV-EGFP-TBK1-E696K

Plasmid

#198037

Purpose

Used for the expression and purification of EGFP labelled TBK1 carrying E696K mutation (SMC Internal No.: 1649)

Depositor

Sascha Martens

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Insert

TBK1 (TBK1 Human)

Use

Tags

GST-TEV-EGFP

Expression

Insect

Mutation

E696K

Promoter

Available since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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pFastBac-GST-TEV-mCherry-TBK1

Plasmid

#198033

Purpose

Used for the expression and purification of mCherry labelled TBK1 (SMC Internal No.: 1675)

Depositor

Sascha Martens

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Insert

TBK1 (TBK1 Human)

Use

Tags

GST-TEV and mCherry

Expression

Insect

Mutation

E696K

Promoter

Available since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO.1-Teton-puro-shPP1C #2

Plasmid

#198763

Purpose

conditional knockdown of PP1C

Depositor

Hyonchol Jang

Article

Kim et al Cancers (Basel). 2020 Sep 11;12(9):2601.

Insert

shPP1C #2 (PPP1CC Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO.1-Teton-puro-shPP1C #1

Plasmid

#198762

Purpose

conditional knockdown of PP1C

Depositor

Hyonchol Jang

Article

Kim et al Cancers (Basel). 2020 Sep 11;12(9):2601.

Insert

shPP1C #1 (PPP1CC Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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Sniper2P

Plasmid

#193857

Purpose

Expresses human codon-optimized Sniper2P and blasticidin resistance: EFS promoter-Sniper2P-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Chem Biol. 2023 Mar 9. doi: 10.1038/

Insert

Sniper2L

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Mutation

E1007L

Promoter

EFS

Available since

March 24, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmAGO1-del942-971-GGSG_M

Plasmid

#146950

Purpose

Insect Expression of DmAGO1-del942-971-GGSG

Depositor

Elisa Izaurralde

Article

Boland et al Proc Natl Acad Sci U S A. 2011 Jun 28;10

Insert

DmAGO1-del942-971-GGSG (AGO1 Fly)

Use

Tags

Expression

Insect

Mutation

variation with 2 non silent mutations V315A and H…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmAGO1-del942-971-GSG_M

Plasmid

#146951

Purpose

Insect Expression of DmAGO1-del942-971-GSG

Depositor

Elisa Izaurralde

Article

Boland et al Proc Natl Acad Sci U S A. 2011 Jun 28;10

Insert

DmAGO1-del942-971-GSG (AGO1 Fly)

Use

Tags

Expression

Insect

Mutation

variation with 2 non silent mutations V315A and H…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmAGO1-E798AQ800A_M

Plasmid

#146952

Purpose

Insect Expression of DmAGO1-E798AQ800A

Depositor

Elisa Izaurralde

Article

Boland et al Proc Natl Acad Sci U S A. 2011 Jun 28;10

Insert

DmAGO1-E798AQ800A (AGO1 Fly)

Use

Tags

Expression

Insect

Mutation

variation with 2 non silent mutations V315A and H…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmAGO1-E798QQ800E_M

Plasmid

#146953

Purpose

Insect Expression of DmAGO1-E798QQ800E

Depositor

Elisa Izaurralde

Article

Boland et al Proc Natl Acad Sci U S A. 2011 Jun 28;10

Insert

DmAGO1-E798QQ800E (AGO1 Fly)

Use

Tags

Expression

Insect

Mutation

variation with 2 non silent mutations V315A and H…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmAGO1-H941A_M

Plasmid

#146954

Purpose

Insect Expression of DmAGO1-H941A

Depositor

Elisa Izaurralde

Article

Boland et al Proc Natl Acad Sci U S A. 2011 Jun 28;10

Insert

DmAGO1-H941A (AGO1 Fly)

Use

Tags

Expression

Insect

Mutation

variation with 2 non silent mutations V315A and H…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmAGO1-H941K_M

Plasmid

#146955

Purpose

Insect Expression of DmAGO1-H941K

Depositor

Elisa Izaurralde

Article

Boland et al Proc Natl Acad Sci U S A. 2011 Jun 28;10

Insert

DmAGO1-H941K (AGO1 Fly)

Use

Tags

Expression

Insect

Mutation

variation with 2 non silent mutations V315A and H…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmAGO1-H941Q_M

Plasmid

#146956

Purpose

Insect Expression of DmAGO1-H941Q

Depositor

Elisa Izaurralde

Article

Boland et al Proc Natl Acad Sci U S A. 2011 Jun 28;10

Insert

DmAGO1-H941Q (AGO1 Fly)

Use

Tags

Expression

Insect

Mutation

variation with 2 non silent mutations V315A and H…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmAGO1-K690AK694A_M

Plasmid

#146958

Purpose

Insect Expression of DmAGO1-K690AK694A

Depositor

Elisa Izaurralde

Article

Boland et al Proc Natl Acad Sci U S A. 2011 Jun 28;10

Insert

DmAGO1-K690AK694A (AGO1 Fly)

Use

Tags

Expression

Insect

Mutation

variation with 2 non silent mutations V315A and H…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmAGO1-K694A_M

Plasmid

#146959

Purpose

Insect Expression of DmAGO1-K694A

Depositor

Elisa Izaurralde

Article

Boland et al Proc Natl Acad Sci U S A. 2011 Jun 28;10

Insert

DmAGO1-K694A (AGO1 Fly)

Use

Tags

Expression

Insect

Mutation

variation with 2 non silent mutations V315A and H…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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