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Plasmid#79262PurposeExpresses EGFP-tagged DmMextli in S2 cellsDepositorInsertDmMextli (mxt Fly, NM_164598)
UseSchneider 2 cell expressionTagsEGFPExpressionMutationPromoterAc5 promoterAvailable sinceJuly 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1:mTRESK(S116C)
Plasmid#224772PurposeIn vitro transcription for Oocyte expression of mTRESK(S116C)DepositorInsertPotassium channel subfamily member 18 (Kcnk18 Mouse)
UseIn vitro transcription for oocyte expressionTagsExpressionMutationS116CPromoterT7Available sinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIRES2-eGFP:HA-mTREK-1
Plasmid#224773PurposeMammalian expression vector for generating mouse TREK-1DepositorInsertPotassium channel subfamily K member 2 (Kcnk2 Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE:mTREK-1(S131C)
Plasmid#224744PurposeIn vitro transcription for Oocyte expression of mTREK-1(S131C)DepositorInsertPotassium channel subfamily K member 2 (Kcnk2 Mouse)
UseIn vitro transcription for oocyte expressionTagsExpressionMutationS131CPromoterT7Available sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE:mTREK-1(S131A)
Plasmid#224745PurposeIn vitro transcription for Oocyte expression of mTREK-1(S131A)DepositorInsertPotassium channel subfamily K member 2 (Kcnk2 Mouse)
UseIn vitro transcription for oocyte expressionTagsExpressionMutationS131APromoterT7Available sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE:mTREK-1(G171F)
Plasmid#224747PurposeIn vitro transcription for Oocyte expression of mTREK-1(G171F)DepositorInsertPotassium channel subfamily K member 2 (Kcnk2 Mouse)
UseIn vitro transcription for oocyte expressionTagsExpressionMutationG171FPromoterT7Available sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE:mTREK-1(A286F)
Plasmid#224746PurposeIn vitro transcription for Oocyte expression of mTREK-1(A286F)DepositorInsertPotassium channel subfamily K member 2 (Kcnk2 Mouse)
UseIn vitro transcription for oocyte expressionTagsExpressionMutationA286FPromoterT7Available sinceSept. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDonor-PRF1-T2A-cDNA
Plasmid#209076PurposeTargeting vector for the human PRF1 locus to replace exon 3 with repaired cDNADepositorInsertPRF1-T2A-cDNA HDRT (PRF1 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-HaloTag-KRas4B(CT)
Plasmid#214276PurposeMammalian expression of HaloTag fused to the C-terminal tail of KRas4BDepositorArticleInsertHaloTag7-KRas4B(174-188) (KRAS Human)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX330_SAFBgRNA8
Plasmid#196103PurposeContains guide RNA to 3' end of mouse SAFB gene for safb1/2 dko. Used with Addgene IDs: 196106, 196107, 196108DepositorInsertSAFB (Safb Mouse)
UseCRISPR and Mouse TargetingTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
tetO-NFYB
Plasmid#170697Purposedoxycycline-inducible overexpression of NFYBDepositorInsertNYFB (NFYB Human)
UseLentiviral; Doxycycline inducibleTagsExpressionMammalianMutationPromoterTRE promoter, Tet-ONAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDECKO_mCherry_NFE2
Plasmid#140105PurposeCRISPR-Cas9 Knock downDepositorInsertgRNA1+scaffold+H1promoter+gRNA2
UseLentiviralTagsExpressionMutationPromotergRNA1 under U6 and gRNA2 under H1Available sinceApril 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDECKO_mCherry_TREML2
Plasmid#140106PurposeCRISPR-Cas9 Knock downDepositorInsertgRNA1+scaffold+H1promoter+gRNA2
UseLentiviralTagsExpressionMutationPromotergRNA1 under U6 and gRNA2 under H1Available sinceApril 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDECKO_mCherry_LINC02432
Plasmid#140107PurposeCRISPR-Cas9 library validationDepositorInsertgRNA1+scaffold+H1promoter+gRNA2
UseLentiviralTagsExpressionMutationPromotergRNA1 under U6 and gRNA2 under H1Available sinceApril 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDECKO_mCherry_MIR3945HG
Plasmid#140108PurposeCRISPR-Cas9 library validationDepositorInsertgRNA1+scaffold+H1promoter+gRNA2
UseLentiviralTagsExpressionMutationPromotergRNA1 under U6 and gRNA2 under H1Available sinceApril 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDECKO_mCherry_Intergenic
Plasmid#140100PurposeCRISPR-Cas9 library validation (negative control)DepositorInsertgRNA1+scaffold+H1promoter+gRNA2
UseLentiviralTagsExpressionMutationPromotergRNA1 under U6 and gRNA2 under H1Available sinceApril 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDECKO_mCherry_FURIN
Plasmid#140103PurposeCRISPR-Cas9 library validationDepositorInsertgRNA1+scaffold+H1promoter+gRNA2
UseLentiviralTagsExpressionMutationPromotergRNA1 under U6 and gRNA2 under H1Available sinceApril 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
ARB366
Plasmid#124049PurposeEncodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a expressing sadCas9 fused to KRAB domain P2A mRuby2, and an sgRNA targeting pUAS expressing mAzamiGreen in a PiggyBac destination vectorDepositorInsertPB_pEF1a-tet3G-P2A-iRFP713_pEF1a-sadCAS9::KRAB-P2A-mRuby2-SV40PA_mu6-sgUAS_pUAS-NLS::mAzamiGreen-rgPA
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
ARB367
Plasmid#124050PurposeEncodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a expressing sadCas9 fused to VPR domain P2A mRuby2, and an sgRNA targeting pUAS expressing mAzamiGreen in a PiggyBac destination vectorDepositorInsertPB_pEF1a-tet3G-P2A-iRFP713_pEF1a-sadCAS9::VPR-P2A-mRuby2-SV40PA_mu6-sgUAS_pUAS-NLS::mAzamiGreen-rgPA
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
tetO-GATA6
Plasmid#170688Purposedoxycycline-inducible overexpression of GATA6DepositorInsertGATA6 (GATA6 Human)
UseLentiviral; Doxycycline inducibleTagsExpressionMammalianMutationPromoterTRE promoter, Tet-ONAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only