-
Plasmid#179293PurposeE. coli expression plasmid (T7 promoter) for full-length human G0S2 with N-terminal His6 + SUMO + TEV protease cleavage siteDepositorInsertG0S2 (G0S2 Human)
UseTagsHis6 + SUMO + TEV protease cleavage siteExpressionBacterialMutationfull-length G0S2, residues 1-103 from accession n…PromoterT7Available sinceFeb. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEMS1132
Plasmid#29053PurposeHigh copy homologous recombination plasmid for gene targeting at the mouse Hprt locus containing Ple67 (FEV MiniPromoter) driving an EGFP/Cre fusion reporterDepositorInsertPle67 (FEV Human)
UsePleiades promoter project [sic, pleaides plieades]TagsEGFP-Cre-NLSExpressionMutationPromoterAvailable sinceJan. 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLenti-EML4-ALK variant 3a I1171N
Plasmid#183832PurposeExpress EML4-ALK fusion variant 3a (E6a;A20) harboring ALK I1171N mutation in mammalian cellsDepositorUseLentiviralTagsThe fusion protein of EML4 exon 1-6a and ALK exon…ExpressionMammalianMutationI1171NPromoterCMVAvailable sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CD74-ROS1 S1986F/G2032R/L2086F
Plasmid#183822PurposeExpress CD74-ROS1 fusion (C6;R34) harboring ROS1 S1986F/G2032R/L2086F mutation in mammalian cellsDepositorUseLentiviralTagsThe fusion protein of CD74 exon 1-6 and ROS1 exon…ExpressionMammalianMutationS1986F/G2032R/L2086FPromoterCMVAvailable sinceApril 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CD74-ROS1 G2032R/L2086F
Plasmid#183820PurposeExpress CD74-ROS1 fusion (C6;R34) harboring ROS1 G2032R/L2086F mutation in mammalian cellsDepositorUseLentiviralTagsThe fusion protein of CD74 exon 1-6 and ROS1 exon…ExpressionMammalianMutationG2032R/L2086FPromoterCMVAvailable sinceApril 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CD74-ROS1 S1986F/G2032R
Plasmid#183821PurposeExpress CD74-ROS1 fusion (C6;R34) harboring ROS1 S1986F/G2032R mutation in mammalian cellsDepositorUseLentiviralTagsThe fusion protein of CD74 exon 1-6 and ROS1 exon…ExpressionMammalianMutationS1986F/G2032RPromoterCMVAvailable sinceApril 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CD74-ROS1 S1986F/L2000V
Plasmid#183818PurposeExpress CD74-ROS1 fusion (C6;R34) harboring ROS1 S1986F/L2000V mutation in mammalian cellsDepositorUseLentiviralTagsThe fusion protein of CD74 exon 1-6 and ROS1 exon…ExpressionMammalianMutationS1986F/L2000VPromoterCMVAvailable sinceApril 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CD74-ROS1 S1986F/L2086F
Plasmid#183819PurposeExpress CD74-ROS1 fusion (C6;R34) harboring ROS1 S1986F/L2086F mutation in mammalian cellsDepositorUseLentiviralTagsThe fusion protein of CD74 exon 1-6 and ROS1 exon…ExpressionMammalianMutationS1986F/L2086FPromoterCMVAvailable sinceApril 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-EML4-ALK variant 3a I1171N/D1203N
Plasmid#183833PurposeExpress EML4-ALK fusion variant 3a (E6a;A20) harboring ALK I1171N/D1203N mutation in mammalian cellsDepositorUseLentiviralTagsThe fusion protein of EML4 exon 1-6a and ALK exon…ExpressionMammalianMutationI1171N/D1203NPromoterCMVAvailable sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
MoChlo: Modular Cloning Chloroplast Toolbox
Plasmid Kit#1000000156PurposeThe MoChlo Toolbox consists of a library of standardized chloroplast-specific genetic modules for Golden-Gate cloning of synthetic operons into chloroplast transformation vectors.DepositorAvailable sinceAug. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
BJ5183 cells
Bacterial Strain#16398DepositorBacterial ResistanceStreptomycinSpeciesEscherichia coliAvailable sinceJune 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
pTKA001
Plasmid#164577PurposeExpresses SARS-CoV-2 nsp7 from a pET46 backbone for IPTG-inducible bacterial expressionDepositorInsertnsp7 (ORF1ab )
UseTags6xHis, Ek, TEVExpressionBacterialMutationPromoterT7 PromoterAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTKA002
Plasmid#164578PurposeExpresses SARS-CoV-2 nsp8 from a pET46 backbone for IPTG-inducible bacterial expressionDepositorInsertnsp8
UseTags6xHis, Ek, TEVExpressionBacterialMutationPromoterT7 PromoterAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
V. natriegens NC7
Bacterial Strain#215356PurposeVibrio natriegens with genomically integrated natural competence regulator, TfoX. For zero-capital molecular biology applications.DepositorBacterial ResistanceNoneAvailable sinceFeb. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
V. natriegens NC1
Bacterial Strain#215355PurposeVibrio natriegens with genomically integrated natural competence regulator, TfoX, with chloramphenicol resistance. For zero-capital molecular biology applications.DepositorBacterial ResistanceNoneAvailable sinceFeb. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
FNL Combinatorial Cloning Platform
Plasmid Kit#1000000211PurposeA collection of Multisite Gateway Entry clone plasmids which can be used in a combinatorial fashion to generate large numbers of tagged expression clones for protein expression in a variety of systemsDepositorAvailable sinceDec. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGJK_His-SUMO-LbuCas13a
Plasmid#172488PurposeBacterial expression of codon-optimized His-SUMO-LbuCas13a.DepositorInsertbdSUMO-LbuCas13a (codon-optimized)
UseTagsHis6 and bdSUMOExpressionBacterialMutationPromoterT7 promoter and lac operatorAvailable sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
-
-
Top7 H1-H2
Plasmid#12479DepositorInsertTop7 H1-H2
UseTags6xHisExpressionBacterialMutationH1-H2PromoterAvailable sinceMarch 13, 2007AvailabilityAcademic Institutions and Nonprofits only