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Showing: 9841 - 9860 of 18524 results

  3. R4pGWB601_UBQ10p-Turbo-NES-YFP

    Plasmid
    #127366
    Purpose
    Binary vector for expressing cytosolic TurboID-YFP under the UBQ10 promoter in plants
    Depositor
    Dominique Bergmann
    Article
    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1
    Insert
    TurboID (BirA mutant)
    Use
    Tags
    GS linker, NES, V5, and YFP
    Expression
    Plant
    Mutation
    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…
    Promoter
    Arabidopsis UBQ10 promoter
    Available since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    127366-plasmid-map-sequence-id-246342

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  5. pSL1145 (pSPIN, pBBR1 backbone, R*MmeI)

    Plasmid
    #160736
    Purpose
    Single-plasmid V. cholerae CAST, encodes all proteins, crRNA, and donor DNA. Non-targeting crRNA with BsaI sites for spacer cloning. Mini-tn has MmeI site in R end for Tn-seq. pBBR1 backbone.
    Depositor
    Samuel H. Sternberg
    Article
    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103
    Insert
    VchCAST proteins, crRNA, and donor DNA
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119
    Available since
    March 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    160736-plasmid-map-sequence-id-326992

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  7. R4pGWB601_UBQ10p-miniTurbo-NES-YFP

    Plasmid
    #127369
    Purpose
    Binary vector for expressing cytosolic miniTurbo-YFP under the UBQ10 promoter in plants
    Depositor
    Dominique Bergmann
    Article
    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1
    Insert
    miniTurbo (BirA mutant)
    Use
    Tags
    GS linker, NES, V5, and YFP
    Expression
    Plant
    Mutation
    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …
    Promoter
    Arabidopsis UBQ10 promoter
    Available since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    127369-plasmid-map-sequence-id-246339

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  14. pU6-Universal.Sth.dCas9

    Plasmid
    #171088
    Purpose
    CRISPR interference. Recipient construct for cloning 20-nt spacers into the sgRNA scaffold compatible with Streptococcus thermophilus dCas9 (CRISPR1 system) via BsaI restriction sites.
    Depositor
    Sebastian Lourido
    Article
    Markus et al mSphere. 2021 Oct 13:e0047421. doi: 10.1
    Inserts
    Toxoplasma U6 upstream region – spacer cloning site - sgRNA scaffold (compatible with S. thermophilus Cas9 CRISPR1)
    DHFR-TSc3
    Use
    Toxoplasma gondii expression
    Tags
    Expression
    Mutation
    Note: A S245F mutation is present but did not app…
    Promoter
    DHFR-TS (Toxoplasma gondii) and U6 (Toxoplasma go…
    Available since
    Oct. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    171088-plasmid-map-sequence-id-335785

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  18. pDF0582 huDisCas7-11 R1045-GGGS-R1122 U6-NT guide

    Plasmid
    #186991
    Purpose
    AAV transgene plasmid for Cas7-11-R1045-GGGS-R1122, with U6 promoter-driven expression of non-targeting crRNA guide
    Depositor
    Omar Abudayyeh, Jonathan Gootenberg
    Article
    Kato et al Cell. 2022 May 25. pii: S0092-8674(22)00
    Insert
    cas7-11-r1045-gggs-r1122, non-targeting crRNA guide
    Use
    AAV
    Tags
    Expression
    Mutation
    r1045-gggs-r1122 deletion
    Promoter
    Available since
    Sept. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    186991-plasmid-map-sequence-id-370002

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  19. pSW646 - pML1>>

    Plasmid
    #115995
    Purpose
    destination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expression
    Depositor
    Sebastien Wolf
    Article
    Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0
    Insert
    pML1:B-dummy:GR-LhG4:D-dummy:tRBCS:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
    Use
    Tags
    HDEL and SP(ER)
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    115995-plasmid-map-sequence-id-227319

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Showing: 9841 - 9860 of 18524 results