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Plasmid#48221PurposedCas9VP160 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expressionDepositorInsertdCas9(D10A;H840A) fusion with VP160 activation domain
UseCRISPR; Gateway donorTagsHA Tag and VP160ExpressionMutationD10A;H840A nuclease-deficientPromoterAvailable sinceSept. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTBRII / CEP-Zeo / Hygro
Plasmid#16622DepositorInsertTGF beta type II receptor (TGFBR2 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceMarch 24, 2008AvailabilityAcademic Institutions and Nonprofits only -
pX330A-Yap1-E1-#1
Plasmid#171517Purposedeletion of a genomic locus in Yap1 geneDepositorInsertYap1 (Yap1 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX330A-Yap1-E2-#1
Plasmid#171518Purposedeletion of a genomic locus in Yap1 geneDepositorInsertYap1 (Yap1 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
(194) pcDNA3.1-HA-nEPEA-(EAAAK)4-OGT(4,K852A)
Plasmid#160742PurposeExpresses an inactive truncated OGT (4.5 TPRs, K852A) that can't bind O-GlcNAc fused to the EPEA nanobody. A different nanobody can be inserted by using Sgfl + Sgsl restriction enzymes.DepositorInsertHA-nEPEA-(EAAAK)4-OGT(4,K852A)
UseTagsHA-TagExpressionMammalianMutationPromoterT7/CMVAvailable sinceMay 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pECE-M2-AMPKalpha2 M93G
Plasmid#31653DepositorInsertprotein kinase, AMP-activated, alpha 2 catalytic subunit M93G (PRKAA2 Human)
UseTagsM2ExpressionMammalianMutationchanged Methionine 93 to GlycinePromoterAvailable sinceApril 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDECKO_mCherry_CEACAM1
Plasmid#140102PurposeCRISPR-Cas9 library validationDepositorInsertgRNA1+scaffold+H1promoter+gRNA2
UseLentiviralTagsExpressionMutationPromotergRNA1 under U6 and gRNA2 under H1Available sinceApril 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
JPF0535a
Plasmid#124042PurposeEncodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a expressing spdCas9 fused to tagBFP P2A tagBFP, and an sgRNA targeting pTRE expressing mAzamiGreen in a PiggyBac destination vectorDepositorInsertPB_pEF1a-tet3G-P2A-iRFP713_pEF1a-spdCAS9::tagBFP-P2A-tagBFP-SV40PA_mu6-sgTRE_pTRE-NLS::mAzamiGreen-rgPA
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDECKO_mCherry_Rat_cebp-alfa
Plasmid#140098PurposeCRISPR-Cas9 library validation (positive control)DepositorInsertgRNA1+scaffold+H1promoter+gRNA2
UseLentiviralTagsExpressionMutationPromotergRNA1 under U6 and gRNA2 under H1Available sinceApril 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDECKO_mCherry_SPI1
Plasmid#140099PurposeCRISPR-Cas9 library validation (positive control)DepositorInsertgRNA1+scaffold+H1promoter+gRNA2
UseLentiviralTagsExpressionMutationPromotergRNA1 under U6 and gRNA2 under H1Available sinceApril 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
eSpCas9-EF-5Nanog_2
Plasmid#174871PurposeCRISPR vector for generating Nanog STREAMING-tag KI cellDepositorInsertsgRNA for mouse Nanog (Nanog Synthetic)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pECE-HA-AMPKalpha2 M93G
Plasmid#31655DepositorInsertprotein kinase, AMP-activated, alpha 2 catalytic subunit M93G (PRKAA2 Human)
UseTagsHA (YDVPDYASL)ExpressionMammalianMutationchanged Methionine 93 to GlycinePromoterAvailable sinceApril 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
MAC_C_HSP7C
Plasmid#111667PurposeMAC-tagged gene expressionDepositorInsertHSP7C (HSPA8 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
p.UTA.2.0 miR-27a-3p 3 perfect copies.
Plasmid#82439PurposeDual Fluorescent endogenous miRNA sensor. Perfect complementary region of hsa-miR-27a-3p in three copiesDepositorInserthsa-miR-27a-3p (MIR27A Human)
UseTagsExpressionMutationThree copies hsa-miR-27a-3p Complementary region …PromoterAvailable sinceMarch 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
200 bp Axin2 mutant
Plasmid#25704DepositorInsertAxin 2 promoter (AXIN2 Human)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationTCF DNA binding site at bp -108 to -102 mutated …PromoterAvailable sinceJune 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
FR_HNF1B_A263insGG
Plasmid#31103DepositorInsertHNF1B (HNF1B Human)
UseFlp/frtTagsmycExpressionMammalianMutationmutation A263insGGPromoterAvailable sinceOct. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
ARB365
Plasmid#124048PurposeEncodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a expressing sadCas9 fused to mRuby2 P2A mRuby2, and an sgRNA targeting pUAS expressing mAzamiGreen in a PiggyBac destination vectorDepositorInsertPB_pEF1a-tet3G-P2A-iRFP713_pEF1a-sadCAS9::mRuby2-P2A-mRuby2-SV40PA_mu6-sgUAS_pUAS-NLS::mAzamiGreen-rgPA
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
FR_HNF1B_P328L329del
Plasmid#31102DepositorInsertHNF1B (HNF1B Human)
UseFlp/frtTagsmycExpressionMammalianMutationmutation P328L329delCCTCTPromoterAvailable sinceOct. 28, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGFP AH2 tensin
Plasmid#50514PurposeThis plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.DepositorInsertTensin (TNS1 Chicken)
UseTagsGFPExpressionMammalianMutationAA659--761, including actin homology 2 domain of …PromoterCMVAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFL-SV40-p38 β/MAPK11 5'-UTR
Plasmid#115357Purposefirefly luciferase (Fluc) reporter containing the 5’-UTR of p38 β/MAPK11 mRNADepositorInsertp38 β/MAPK11 5'-UTR (MAPK11 Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 13, 2018AvailabilityAcademic Institutions and Nonprofits only