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Plasmid#177578Purposeexpressing SARS-CoV-2 B.1.621 (Mu) spike protein for pseudovirus productionDepositorInsertSpike (Mu variant) (S SARS-CoV-2)
UseRetroviralTagsExpressionMammalianMutationT95I, Y144T, Y145S, R346K, E484K, N501Y, D614G, …PromoterCMVAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3_SARS2_omicron_BA.2.75
Plasmid#190674Purposeexpressing SARS-CoV-2 BA.2.75 spike protein for pseudovirus productionDepositorInsertSpike (S SARS-CoV-2)
UseTagsExpressionMammalianMutationK147E, W152R, F157L, I210V, G257S, D339H, G446S, …PromoterCMVAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3_SARS2_Lambda
Plasmid#177579Purposeexpressing SARS-CoV-2 C.37 (lambda) spike protein for pseudovirus productionDepositorInsertSpike (Lambda variant) (S SARS-CoV-2)
UseRetroviralTagsExpressionMammalianMutation75-76VI, 246-253N, L452Q, F490S, D614G, T859N, la…PromoterCMVAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL4-eIF3B
Plasmid#133308PurposeExpression of luciferase driven by eIF3B promoter regionDepositorInserteIF3B promoter (EIF3B Human)
UseLuciferaseTagsExpressionMammalianMutationPromotereIF3BAvailable sinceOct. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CD74-ROS1 G2032R
Plasmid#183814PurposeExpress CD74-ROS1 fusion (C6;R34) harboring ROS1 G2032R mutation in mammalian cellsDepositorUseRetroviralTagsThe fusion protein of CD74 exon 1-6 and ROS1 exon…ExpressionMammalianMutationG2032RPromoterCMVAvailable sinceApril 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-FRT/TO-eGFP-REV7
Plasmid#114128PurposeExpresses N-terminally tagged eGFP-REV7 in mammalian cellsDepositorInsertREV7 (MAD2L2 Human)
UseGenomic integration, tetracycline inducibleTagseGFPExpressionMammalianMutationPromoterCMV/TetO2Available sinceAug. 15, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCW-eGFP-FHA-SHLD2-m1
Plasmid#114122PurposeLentiviral vector for inducible expression of N-terminally tagged eGFP-RNF8 FHA domain-SHLD2, m1 mutant; 53BP1-independent recruitment to damaged chromatinDepositorInsertSHLD2 (m1 mutant) (SHLD2 Human)
UseLentiviral; Doxycycline inducibleTagsRNF8 FHA domain (aa 2-160) and eGFPExpressionMammalianMutationW489A, F494A, W495A mutations introducedPromoterTRE promoter, Tet ONAvailable sinceAug. 28, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSLIK-NFLAG-hEWSR1G511A-CMyc
Plasmid#50734PurposeProduces lentivirus expressing human EWSR1G511A mutant with FLAG tag at its N-terminus and Myc tag at its C-terminus by co-transfection with psPAX2 and pMD2GDepositorInsertEWSR1 G511A (EWSR1 Human)
UseLentiviralTagsFLAG and MycExpressionMutationChanged Gly 511 to AlaPromoterTREAvailable sinceJan. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSLIK-NFLAG-hEWSR1P552L-CMyc
Plasmid#50736PurposeProduces lentivirus expressing human EWSR1P552L mutant with FLAG tag at its N-terminus and Myc tag at its C-terminus by co-transfection with psPAX2 and pMD2GDepositorInsertEWSR1 P552L (EWSR1 Human)
UseLentiviralTagsFLAG and MycExpressionMutationChanged Pro 552 to LeuPromoterTREAvailable sinceJan. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CD74-ROS1 L2086F
Plasmid#183816PurposeExpress CD74-ROS1 fusion (C6;R34) harboring ROS1 L2086F mutation in mammalian cellsDepositorUseLentiviralTagsThe fusion protein of CD74 exon 1-6 and ROS1 exon…ExpressionMammalianMutationL2086FPromoterCMVAvailable sinceApril 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CD74-ROS1 L2000V
Plasmid#183815PurposeExpress CD74-ROS1 fusion (C6;R34) harboring ROS1 L2000V mutation in mammalian cellsDepositorUseLentiviralTagsThe fusion protein of CD74 exon 1-6 and ROS1 exon…ExpressionMammalianMutationL2000VPromoterCMVAvailable sinceApril 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLIK-NFLAG-hEWSR1WT-CMyc
Plasmid#50733PurposeProduces lentivirus expressing human EWSR1 wild type with FLAG tag at its N-terminus and Myc tag at its C-terminus by co-transfection with psPAX2 and pMD2GDepositorInsertEWSR1 (EWSR1 Human)
UseLentiviralTagsFLAG and MycExpressionMutationPromoterTREAvailable sinceJan. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
HisGB1-Fusion + CBP TAZ1(340-439)
Plasmid#173762PurposeBacterial coexpression of CBP TAZ1 and CITED2/Hif1a activation domain constructsDepositorUseTagsHis6GB1 and thrombinExpressionBacterialMutationfusion of CITED2 and Hif1aPromoterT7Available sinceFeb. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
HisGB1-FusionL21A + CBP TAZ1(340-439)
Plasmid#173763PurposeBacterial Coexpression of CBP TAZ1 with CITED2/Hif1a activation domain constructsDepositorUseTagsHis6GB1 and thrombinExpressionBacterialMutationfusion of CITED2 and Hif1aPromoterT7Available sinceFeb. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
HIsGB1-FusionL63A + CBP TAZ1(340-439)
Plasmid#173764PurposeBacterial coexpression of CBP TAZ1 with CITED2/Hif1a activation domain constructsDepositorUseTagsHis6GB1 and thrombinExpressionBacterialMutationfusion of CITED2 and Hif1aPromoterT7Available sinceFeb. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGL4-eIF3B MBE3 mutant
Plasmid#133310PurposeExpression of luciferase driven by eIF3B promoter region mutated at the E-box binding motif 3DepositorInserteIF3B promoter MBE3 mutant (EIF3B Human)
UseLuciferaseTagsExpressionMammalianMutationccacgtgacc changed to cAaAAAAaccPromotereIF3BAvailable sinceOct. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL4-eIF3B MBE2 mutant
Plasmid#133309PurposeExpression of luciferase driven by eIF3B promoter region mutated at the E-box binding motif 2DepositorInserteIF3B promoter MBE2 mutant (EIF3B Human)
UseLuciferaseTagsExpressionMammalianMutationgccacatgcacc changed to gcAaAaAAcaccPromotereIF3BAvailable sinceOct. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-TAF15 g1172a No Stop
Plasmid#84898PurposeDONOR vector for Gateway cloning of TAF15 g1172a No StopDepositorInsertTAF15 (TAF15 Human)
UseGateway donorTagsExpressionMutationg1172a No StopPromoternoneAvailable sinceApril 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-TAF15 c1222t No Stop
Plasmid#84900PurposeDONOR vector for Gateway cloning of TAF15 c1222t No StopDepositorInsertTAF15 (TAF15 Human)
UseGateway donorTagsExpressionMutationc1222t No StopPromoternoneAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
6xHis-MBP-BrCas12b-FNLDTA
Plasmid#195340PurposeExpresses Engineered BrCas12b in BacteriaDepositorInsertBrCas12b
UseCRISPR and Synthetic BiologyTags6xHis Tag and MBP, TEV cleavage site, 6x His TagExpressionBacterialMutationF208W, N524V, L795I, D868V, T874S, A1015EPromoterT7 PromoterAvailable sinceMay 5, 2023AvailabilityAcademic Institutions and Nonprofits only