Addgene

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pMOD_A0801

Plasmid

#91022

Purpose

Module A, Promoter: 35S, Gene: Csy4-P2A-AtCas9_dead (D10A + H840A), Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Csy4-P2A-AtCas9_dead (D10A + H840A)

Use

CRISPR

Tags

Expression

Mutation

D10A, H840A

Promoter

35S

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pETM41-EcLonA

Plasmid

#38330

Depositor

Florian Freimoser

Article

Werner et al Fungal Genet Biol. 2007 Sep;44(9):845-52

Insert

Lon protease fragment (lon Escherichia coli)

Use

Tags

His, MBP, and TEV

Expression

Bacterial

Mutation

Polyphosphate binding fragment of Lon protease. …

Promoter

T7

Available since

Aug. 10, 2012

Availability

Academic Institutions and Nonprofits only
PLK3 1-334-pEGFP

Plasmid

#23266

Depositor

Wei Dai

Article

Yang et al Cancer Res. 2008 Jun 1. 68(11):4077-85.

Insert

PLK3 (PLK3 Human)

Use

Tags

pEGFP

Expression

Mammalian

Mutation

aa 1-334

Promoter

Available since

Dec. 20, 2012

Availability

Academic Institutions and Nonprofits only
pCMV-Flag-YAP-4SA(S61)

Plasmid

#33080

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2010 Jan 1. 24(1):72-85.

Insert

YAP (YAP1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

S109A, S127A, S164A, S381A

Promoter

CMV

Available since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
pICSL80036

Plasmid

#68259

Purpose

Level 0 Golden Gate Part: Coding sequence, hygromycin phophotransferase II (Escherichia coli)

Depositor

Nicola Patron

Article

Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

Insert

Coding sequence, hygromycin phophotransferase II (Escherichia coli)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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mEos2-Alpha-5-Integrin-12

Plasmid

#57344

Purpose

Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

Alpha-5 Integrin (ITGA5 Human)

Use

Tags

mEos2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pFAB822

Plasmid

#47856

Purpose

BIOFAB reporter plasmid for measuring BBa_B1006 termination efficiency

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

BBa_B1006 terminator

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

pLTetO11

Available since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pFAB803

Plasmid

#47847

Purpose

BIOFAB reporter plasmid for measuring T7 early termination efficiency

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

T7 early terminator

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

pLTetO2

Available since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only

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MSCV-miRE_shBRD9_561-SV40-GFP

Plasmid

#75139

Purpose

Retroviral expression plasmid encoding shBRD9_561 (targeting human BRD)

Depositor

Christopher Vakoc

Article

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

shBRD9_561

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

May 23, 2016

Availability

Academic Institutions and Nonprofits only
bdSUMO-R9-GFP-HiBit

Plasmid

#170990

Purpose

For bacterial expression of bdSUMO tagged muGFP with R9 peptide and NanoLuc complementation peptide variant #86

Depositor

Angus Johnston

Article

Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

Insert

14x-His-bdSUMO-R9-muGFP-HiBiT

Use

Luciferase

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pBABE-Hygro HoxB9

Plasmid

#27022

Depositor

Joan Massague

Article

Nguyen et al Cell. 2009 Jul 10. 138(1):51-62.

Insert

HoxB9 (HOXB9 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 17, 2010

Availability

Academic Institutions and Nonprofits only
pGoldyTALEN-TUBA1B-TAL-2

Plasmid

#62854

Purpose

TALEN mediated gene editing at human TUBA1B locus

Depositor

Gaudenz Danuser

Article

Gan et al Cell Syst. 2016 Sep 21. pii: S2405-4712(

Insert

TALEN array

Use

TALEN

Tags

Expression

Mammalian

Mutation

Promoter

miniCAGG

Available since

May 27, 2015

Availability

Academic Institutions and Nonprofits only

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pGoldyTALEN-TUBA1B-TAL-1

Plasmid

#62853

Purpose

TALEN mediated gene editing at human TUBA1B locus

Depositor

Gaudenz Danuser

Article

Gan et al Cell Syst. 2016 Sep 21. pii: S2405-4712(

Insert

TALEN array

Use

TALEN

Tags

Expression

Mammalian

Mutation

Promoter

miniCAGG

Available since

May 27, 2015

Availability

Academic Institutions and Nonprofits only

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GAL4-mNFAT1(2-144)

Plasmid

#11108

Purpose

Expresses mouse NFAT1 (amino acids 2-144) fused to GAL4 DNA binding domain in mammalian cells

Depositor

Anjana Rao

Article

Garcia-Rodriguez et al Eur J Immunol. 2000 Aug . 30(8):2432-6.

Insert

NFAT1 (Nfatc2 Mouse)

Use

Tags

GAL4-DBD

Expression

Mammalian

Mutation

Contains NFAT1 amino acids 2-144; L78P and P140Q …

Promoter

Available since

April 24, 2006

Availability

Academic Institutions and Nonprofits only

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pmcsg7-mCRT(WT)

Plasmid

#83505

Purpose

Bacterial expression of Calreticulin

Depositor

Malini Raghavan

Article

Del Cid et al J Biol Chem. 2010 Feb 12;285(7):4520-35.

Insert

CALR (Calr Mouse)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 12, 2016

Availability

Academic Institutions and Nonprofits only
mTagBFP2-CD36-C-10

Plasmid

#55280

Purpose

Localization: Membrane, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

CD36 (CD36 Human)

Use

Tags

mTagBFP2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 22, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pLKO.1-sh-mSox10-2

Plasmid

#37007

Depositor

Bob Weinberg

Article

Guo et al Cell. 2012 Mar 2;148(5):1015-28.

Insert

sh-mSox10-2 (Sox10 Mouse)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

May 31, 2012

Availability

Academic Institutions and Nonprofits only
pExT

Plasmid

#36889

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Inserts

T (i.e., ATG, start codon as a first exon)
GFP
beta-globin intron
Hygro

Use

Tags

Expression

Mammalian

Mutation

deleted nucleotides before nucleotide 275 and ins…

Promoter

CAG (chicken beta actin promoter and CMV enhancer…

Available since

Aug. 8, 2012

Availability

Academic Institutions and Nonprofits only

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pSBL_DK193

Plasmid

#156424

Purpose

Insect cell expression of 3a(Q57H)-sfGFP, needs to be introduced into baculoviral bacmid

Depositor

Stephen Brohawn

Article

Kern et al bioRxiv [Preprint]. 2021 Jan 26:2020.06.

Insert

SARS-CoV-2 3a protein (Q57H)

Use

Tags

sfGFP-His

Expression

Insect

Mutation

Q57H

Promoter

Polyhedrin

Available since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pExG

Plasmid

#36888

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Inserts

GFP
tdTomato-3Myc
beta-globin intron
Hygro

Use

Tags

3 Myc tags

Expression

Mammalian

Mutation

deleted nucleotides after nucleotide 274, inserti…

Promoter

CAG (chicken beta actin promoter and CMV enhancer…

Available since

Aug. 17, 2012

Availability

Academic Institutions and Nonprofits only

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