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Plasmid#19514DepositorInsertheat shock 70kDa protein 8 (HSPA8 Human)
UseTagsV5ExpressionMammalianMutationPromoterAvailable sinceOct. 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
pLV CMV mCherry-DDX1-V5
Plasmid#175162PurposeLentiviral expression of human DDX1-V5DepositorInsertDDX1 (DDX1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceJan. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
Chr8q_Centromere-Targeting_gRNA
Plasmid#195128PurposegRNA targeting centromere-proximal location on Chromosome 8q in a third generation Cas9 backbone with GFPDepositorInsertChr8q gRNA
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLIB-GST-3C-CDK1
Plasmid#177011PurposeGenerate baculovirus for insect cell expression of CDK1 with N-terminal GST-3C (PreScission)DepositorInsertCDK1 (CDK1 Human)
UseTagsGST-3CExpressionInsectMutationcodon-optimised for insect cell expressionPromoterAvailable sinceNov. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
TDP-REGv2(Gaussia Luciferase)#5 in pTwist-CMV
Plasmid#216164PurposeExpression of secreted Gaussia Luciferase specifically in cells with TDP-43 loss of functionDepositorInsertGaussia princeps luciferase (with double mutation to improve stability)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
DYRK1A/pTRE3G-FL-hXIST
Plasmid#149608PurposeExpress doxycycline-inducible full length of human XIST cDNADepositorInsertX-inactive specific transcript (XIST Human)
UseTagsExpressionMammalianMutationPromoterpTRE3GAvailable sinceJuly 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDB2-hPER1-mScarlet-I-CD4-bla
Plasmid#189980PurposeDonor Vector to integrate mScarlet-I C-terminal into the human PER1 Locus to express it as a fusion proteinDepositorInsertmScarlet-I
UseCRISPRTags3xFlagExpressionMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLentiCriprV2-sgRNA-PER1-#1
Plasmid#189987PurposeLentiviral Cas9/sgRNA vector targeting C-terminus of hPER1, works with Addgene 189979-189982DepositorInsertPeriod1 (PER1 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
DNAJC11-EGFP
Plasmid#210368PurposeExpresses DNAJC11 fused with EGFP in mammalian cellsDepositorInsertDNAJC11 (DNAJC11 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV/myc/ER-αT9ib
Plasmid#110768PurposeExpresses ER intrabody against mouse and human TLR9DepositorInsertintrabody against mouse and human TLR9
UseTagsER retention signal (SEKDEL), ER signal peptide, …ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
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Flag-ECFP-betaPIXa
Plasmid#15235DepositorInsertbeta-PIXa (Arhgef7 Mouse)
UseTagsECFP and FlagExpressionMammalianMutationPromoterAvailable sinceSept. 13, 2007AvailabilityAcademic Institutions and Nonprofits only -
pBpaRS3tRNA
Plasmid#197102PurposeTo express the p-benzoylphenylalanine-specific variant of Methanocaldococcus jannaschii TyrRS and its cognate amber suppressor tRNA and allow UAG codon to be translated into p-benzoylphenylalanine.DepositorInsertTyrRS variant, amber suppressor tRNA
UseTagsExpressionBacterialMutationGly32, Ser107, Thr158, Ser159, Arg286 (TyrRS)PromoterAvailable sinceJune 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-hChR2(E123T/T159C)-EYFP
Plasmid#35511PurposeAAV expression of CaMKIIa-driven hChR2 variant for ultrafast optogenetic control.DepositorInserthChR2
UseAAVTagsEYFPExpressionMammalianMutationE123T and T159CPromoterCaMKIIaAvailable sinceApril 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCAT-YFP-TetR
Plasmid#59018PurposeExpresses a fusion of yellow fluorescent protein (YFP) to the N-terminus of the Escherichia coli Tn10 tet repressor (TetR) driven by the alpha tubulin promoter for use in T. gondiiDepositorInsertYFP-TetR
UseToxoplasma expressionTagsExpressionMutationPromoteralpha-tubulinAvailable sinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
Fyn-SH3_5WT_S25C
Plasmid#118824PurposeBacterial expression of the SH3 domain of human FynDepositorInsertFYN (FYN Human)
UseTags6-His-TEV tagExpressionBacterialMutationS25C; See depositor comments below.PromoterAvailable sinceApril 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
Chr1q_Centromere-Targeting_gRNA
Plasmid#195125PurposegRNA targeting centromere-proximal location on Chromosome 1q in a third generation Cas9 backbone with GFPDepositorInsertChr1q gRNA
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
YAP1-V5 in pLX304
Plasmid#42555DepositorInsertYAP1 (YAP1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pORTMAGE-4
Plasmid#72679PurposeExpresses Lambda Red recombinases and a dominant negative MutL allele all controlled by temperature sensitve cI857 repressor for high precision and efficiency MAGE experiments. Cam resistance marker.DepositorInsertmutL E32K
UseTagsnoneExpressionBacterialMutationE32K mutation conferring dominant mutator phenoty…PromoterAvailable sinceFeb. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX CrkL-SH2
Plasmid#46419DepositorInsertCrkl (CRKL Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 9-108PromoterTacAvailable sinceJuly 12, 2013AvailabilityAcademic Institutions and Nonprofits only