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Plasmid#84598PurposeRenilla Luciferase reporter assay for BCL2 3'UTR with mutations on miR-200c binding siteDepositorInsertBCL2 3'UTR (UTS2R Human)
UseLuciferaseTagsRenilla LuciferaseExpressionMutationMutation on miR-200c binding site on BCL2 3'…PromoterCMVAvailable sinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCIneoRL-TOB1 3'UTR-mir200c-mut
Plasmid#84590PurposeRenilla Luciferase reporter assay for TOB1 3'UTR with mutations on miR-200c binding siteDepositorInsertTOB1 3'UTR (UTS2R Human)
UseLuciferaseTagsRenilla LuciferaseExpressionMutationMutation on miR-200c binding site on TOB1 3'…PromoterCMVAvailable sinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
mCardinal-Cx26-7
Plasmid#56156PurposeLocalization: Gap Junctions, Excitation: 604, Emission: 659DepositorInsertCx26
UseTagsmCardinalExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3.1-kappa-myc-dL5-2xG4S-TMst
Plasmid#73206PurposeExpresses myc-dL5(E52D)-TM (PDGFR derived) on the surface of mammalian cells, with an Igk-leader. (MBIC5, dL5**, FAP)DepositorInsertkappa-myc-dL5-2XG4S-TM (MYC Human)
UseTagsThe FAP is fused with 2 copies of a G4S linker an…ExpressionMammalianMutationThe dL5** FAP is E50D, L89S, also known as E52D/L…PromoterCMVAvailable sinceMarch 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-NLS-myc-dL5-2xG4S-mCer3
Plasmid#73205PurposeExpresses myc-dL5(E52D)-mCer3 fusion protein in nuclei of mammalian cells. (MBIC5, dL5**, FAP)DepositorInsertNLS-myc-dL5-2XG4S-mCer3 (MYC Synthetic, Human)
UseTagsThe FAP and mCerulean3 are fused with 2 copies of…ExpressionMammalianMutationThe dL5** FAP is E50D, L89S, also known as E52D/L…PromoterCMVAvailable sinceMarch 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
PB09-TRE-beta-globin-miR-ctrl-EF1a-GFP
Plasmid#117319PurposeOverexpression of miR-124-surrounding locus (not miR-124) in eurkaryotic cells, encoded in a human beta-globin intronDepositorInserthsa-miR-CTRL
UseTransposon-mediated integrationTagsGFPExpressionMammalianMutationmiR-124 deletedPromoterTet-inducible promoterAvailable sinceJan. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
Flag-FOXM1
Plasmid#153136PurposeExpression of Flag tagged human FOXM1 in mammalian cellsDepositorInserthuman FOXM1 (FOXM1 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMV IE94Available sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLNCX2-mEGFP-CHMP2B
Plasmid#115329Purposeexpresses mEGFP-CHMP2B in mammalian cellsDepositorInsertCHMP2B (CHMP2B Human)
UseRetroviralTagsmEGFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
mCherry-RNF68
Plasmid#126545PurposeExpresses mCherry-tagged RNF68 in mammalian cellsDepositorInsertRNF68 (PCGF1 Human)
UseRetroviralTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceJuly 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-PDGFRalpha
Plasmid#136434PurposeMammalian expression plasmid for c-myc-tagged PDGFRADepositorInsertPDGFRalpha (PDGFRA Human)
UseTagsExtracellular c-myc epitope tag and Signal peptid…ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-G12-mFGF2
Plasmid#219552PurposemFGF2 expression in mammlian cells. pAAV vector. Reporter plasmid.DepositorInsertmFGF2 (Fgf2 Mouse)
UseAAVTagsExpressionMammalianMutationPromoter12xUAS with TATA-box minimal promoterAvailable sinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
FLAG-FullLength-TET1-LLAA(20)
Plasmid#124397PurposeFull-length mutant TET1 (L897A/L900A) used to PiggyBAC rescue the function of TET1/2 DKO cellsDepositorInsertFLAG-FullLength-TET1 with L897A/L900A mutations (TET1 Human)
UseTagsFlagExpressionMammalianMutationL897A/L900A mutations (full length protein is 213…PromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMH-HT_SsCHAD-H29A-R32A-R36A-R69A
Plasmid#127787PurposeExpresses a SsCHAD mutant protein in E. coli cellsDepositorInsertSsCHAD (SULM_RS11045 Sulfolobus solfataricus)
UseTags6xHis tag + TEV cleavage siteExpressionBacterialMutationmutations in His29, Arg32, Arg36 and Arg69 to AlaPromoterAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGG-GTS-TurboID-GFP
Plasmid#209405PurposeTransiently expressing and visualizing the subcellular localization of TurboID fused with a Golgi targeting sequence (GTS) in plantaDepositorInsertGTS-3XHA-TurboID
UseTagsGFPExpressionPlantMutationTurboID gene sequence C249APromoterCauliflower mosaic virus (CaMV) 35S promoterAvailable sinceJan. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGG-NES-TurboID-GFP
Plasmid#209401PurposeTransiently expressing and visualizing the subcellular localization of TurboID fused with a nuclear export signal (NES) in plantaDepositorInsertNES-3XHA-TurboID
UseTagsGFPExpressionPlantMutationTurboID gene sequence C249APromoterCauliflower mosaic virus (CaMV) 35S promoterAvailable sinceJan. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGG-mTP-TurboID-GFP
Plasmid#209404PurposeTransiently expressing and visualizing the subcellular localization of TurboID fused with a Mitochondrial transit peptide (mTP) in plantaDepositorInsertmTP-3XHA-TurboID
UseTagsGFPExpressionPlantMutationTurboID gene sequence C249APromoterCauliflower mosaic virus (CaMV) 35S promoterAvailable sinceJan. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMH-HT_CtCHAD-H253A-R256A-R260A
Plasmid#127788PurposeExpresses a CtCHAD mutant protein in E. coli cellsDepositorInsertCtCHAD (CT0884 Chlorobium tepidum)
UseTags6xHis tag + TEV cleavage siteExpressionBacterialMutationResidues 208-522 (CHAD domain), mutations in His2…PromoterAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMH-HT_CtCHAD-H253A-R256A-R260A-R296A
Plasmid#127789PurposeExpresses a CtCHAD mutant protein in E. coli cellsDepositorInsertCtCHAD (CT0884 Chlorobium tepidum)
UseTags6xHis tag + TEV cleavage siteExpressionBacterialMutationResidues 208-522 (CHAD domain), mutations in His2…PromoterAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMH-HT_CtCHAD-R385A-K386A-K389A-K390A
Plasmid#127792PurposeExpresses a CtCHAD mutant protein in E. coli cellsDepositorInsertCtCHAD (CT0884 Chlorobium tepidum)
UseTags6xHis tag + TEV cleavage siteExpressionBacterialMutationResidues 208-522 (CHAD domain), mutations in Arg3…PromoterAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcFLAG-Grap2-R83E
Plasmid#74512PurposeOverexpression of Grap2 R83E in mammalian cellsDepositorInsertGrap2 (GRAP2 Human)
UseTagsFLAGExpressionMammalianMutationR83EPromoterCMVAvailable sinceMay 20, 2016AvailabilityAcademic Institutions and Nonprofits only