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Plasmid#54618PurposeLocalization: N1 Cloning Vector, Excitation: 440, Emission: 616DepositorTypeEmpty backboneUseTagsmKeima-RedExpressionMammalianMutationPromoterAvailable sinceJune 12, 2014AvailabilityAcademic Institutions and Nonprofits only
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pKSB- 3xP3-mTurqSV40 GGGG AAGA
Plasmid#174533Purpose3xP3-mTurquoise fluorescence marker cassette for gene knock-in in mosquitoesDepositorInsert3xP3-mTurquoise BsaI cassette
UseTagsExpressionInsectMutationPromoter3xP3Available sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV-U6-gRNA-TnT-Cre
Plasmid#132551PurposeFor loss-of-function experiments in cardiomyocytesDepositorInsertsTnT-Cre
U6-gRNA
UseAAVTagsExpressionMutationPromoterTnT and U6Available sinceOct. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1_V5_BirA*_FLAG_Ct
Plasmid#194071Purpose3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-blastiDepositorTypeEmpty backboneUseLentiviralTagsFLAG-BirA(R118G)-V5ExpressionMammalianMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJ251-GERC
Plasmid#47441PurposeExpresses mCherry and sfGFP from divergent promoters. The backbone is cloned into DNA2.0 pJ251, and the design is based off of pZS2-123.DepositorInsertGene Expression Reporter Construct
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pExp-NHis
Plasmid#112558PurposeUsed for expression of protein in E. coli as TEV cleavable N-terminual 8His fusions with optional C-terminal StrepII-tagDepositorTypeEmpty backboneUseTags8xHis and Strep tagExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1_V5_APEX_FLAG_Nt
Plasmid#194068Purpose3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-blastiDepositorTypeEmpty backboneUseLentiviralTagsV5-APEX-FLAGExpressionMammalianMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-N2cL
Plasmid#100812Purposeexpress rabies virus CVS N2c strain polymerase gene (N2cL)DepositorInsertrabies virus CVS N2c strain polymerase gene
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJune 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-N2cN
Plasmid#100801Purposeexpress rabies virus CVS N2c strain nucleoprotein gene (N2cN)DepositorInsertrabies virus CVS N2c strain nucleoprotein gene
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJune 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-N2cP
Plasmid#100808Purposeexpress rabies virus CVS N2c strain phosphoprotein gene (N2cP)DepositorInsertrabies virus CVS N2c strain phosphoprotein gene
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJune 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pINER5
Plasmid#185895PurposeIn vivo gene amplification (HamAmp) of nerolidol synthetic module at RPL25 locus (15 copy).DepositorInsertPRPL25(Arm1)>Kl.LEU2>TKl.LEU2-PGAL1>ERG20>TRPL3-TRPL25(Arm3)-ARS305-PSk.GAL2>YFAST-EVBR1.2A-AcNES1>TRPL41B-PPDA1>RPL25(part;Arm2
UseTagsExpressionYeastMutationNonePromoterAvailable sinceAug. 25, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTre-Tight-luciferase-GFP (467)
Plasmid#65491Purposenegative control for expression assaysDepositorInsertEGFP
UseLuciferaseTagsExpressionMammalianMutationPromotertight TRE promoterAvailable sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.3_HES4
Plasmid#124072PurposeHES4 with normal stop codonDepositorInsertHES4 (HES4 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKSB- 3xP3-GFPsv40 GGGG AAGA
Plasmid#174531Purpose3xP3-GFP fluorescence marker cassette for gene knock-in in mosquitoesDepositorInsert3xP3-GFP BsaI cassette
UseTagsExpressionInsectMutationPromoter3xP3Available sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti_PGK_Prkaa1
Plasmid#204356PurposeLentiviral plasmid, Expresses Prkaa1 under the control of the PGK promoterDepositorInsertAMP-activated protein kinase alpha 1 (Prkaa1 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterPGKAvailable sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV_Efla_FLEX_APEX2-eGFPf
Plasmid#171939PurposeExpresses a membrane targeted (farnesylation) APEX2-eGFP fusion proteinDepositorInsertAPEX2-eGFPf
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterEf1aAvailable sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
PA-mCherry1-N1
Plasmid#54507PurposeLocalization: N1 Cloning Vector, Excitation: 400 / 504, Emission: 515 / 517DepositorTypeEmpty backboneUseTagsPA-mCherry1ExpressionMammalianMutationPromoterAvailable sinceJune 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
IDG_TMC4_OE_1
Plasmid#161652PurposeOverexpresses TMC4DepositorInsertTMC4 (TMC4 Human)
UseTagsV5ExpressionMammalianMutationPromoterAvailable sinceNov. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-EF1a-GFP-miRE_shRen-Puro
Plasmid#135683PurposeConstitutive expression (EF1a promoter) of GFP cDNA plus miRE-embedded shRenilla (control shRNA), Puromycin selectionDepositorInsertshRenilla
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-EFS-SpCas9-P2A-blast
Plasmid#106477Purposesingle-vector CROPseq system with blasticidin resistance selection for use in the CROPseq CRISPR knockout screensDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEFSAvailable sinceApril 9, 2018AvailabilityAcademic Institutions and Nonprofits only