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pSW646 - pML1>>

Plasmid

#115995

Purpose

destination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expression

Depositor

Sebastien Wolf

Article

Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

Insert

pML1:B-dummy:GR-LhG4:D-dummy:tRBCS:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR

Use

Tags

HDEL and SP(ER)

Expression

Plant

Mutation

Promoter

Available since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-Gluc-RMA-IRES-EGFP

Plasmid

#189630

Purpose

Expresses Gluc-RMA and EGFP in the presence of Cre recombinase. Double-floxed Gluc-RMA and EGFP for monitoring Cre-expressing neuronal populations.

Depositor

Jerzy Szablowski

Article

Lee et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

Inserts

Gaussia luciferase fused to Fc
IRES-EGFP

Use

AAV, Cre/Lox, and Luciferase

Tags

Gluc and IgG1 Fc

Expression

Mammalian

Mutation

Promoter

IRES and hSyn

Available since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-TM-KA2-CaM-NES-TevN-AsLOV2-TEVseq-tTA

Plasmid

#171615

Purpose

Soma-targeted Cal-Light with KA2

Depositor

Hyungbae Kwon

Article

Hyun et al Nat Commun. 2022 Dec 12;13(1):7692. doi:

Insert

TM-KA2-CaM-NES-TevN-AsLOV2-TEVseq-tTA

Use

Synthetic Biology

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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HA-EglN2-pcDNA3

Plasmid

#18961

Depositor

William Kaelin

Article

Lee et al Cancer Cell. 2005 Aug . 8(2):155-67.

Insert

Egg laying Nine - 2 (EGLN2 Human)

Use

Tags

HA-tag

Expression

Mammalian

Mutation

wt cDNA

Promoter

Available since

June 19, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO/GFP-TTP

Plasmid

#141190

Purpose

Expresses GFP-TTP in mammalian cells, can be used to make inducible cell line

Depositor

Simon Bekker-Jensen

Article

Tollenaere et al Cell Rep. 2019 Mar 26;26(13):3511-3521.e

Insert

TTP (ZFP36 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 5, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA pDEST40 Cas9-POLD3

Plasmid

#183194

Purpose

Expression clone

Depositor

Emma Haapaniemi

Article

Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

Insert

POLD3

Use

CRISPR

Tags

Cas9WT

Expression

Mammalian

Mutation

n/a

Promoter

Available since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-E988K

Plasmid

#173945

Purpose

Bacterial expression of inactive PARP1 mutant (E988K attenuates catalytic and PAR-branching activity of PARP1)

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-E988K (PARP1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

V762A, E988K

Promoter

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-C125G

Plasmid

#174792

Purpose

Bacterial expression of a less active PARP1 mutant (C125G reduces affinity for single-strand DNA break)

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-C125G (PARP1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

C125G, V762A

Promoter

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-IFT54

Plasmid

#218726

Purpose

Expresses N-terminally EGFP-tagged IFT54 in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Hiyamizu et al J Cell Sci. 2023 Mar 1;136(5):jcs260462.

Insert

IFT54 (TRAF3IP1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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FR_HNF1B

Plasmid

#31101

Depositor

Gerhart Ryffel

Article

Thomas et al Nucleic Acids Res. 2004 . 32(19):e150.

Insert

HNF1B (HNF1B Human)

Use

Flp/frt

Tags

myc

Expression

Mammalian

Mutation

wild type

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pAAV-SYN-H-sDarken

Plasmid

#184797

Purpose

High affinity version of the Serotonin Sensor sDarken under the control of a hSyn Promoter.

Depositor

Olivia Masseck

Article

Kubitschke et al Nat Commun. 2022 Dec 6;13(1):7525. doi:

Insert

H-sDarken

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pLV Gpat4-V5

Plasmid

#175145

Purpose

Lentiviral expression of mouse Gpat4-V5

Depositor

Larry Gerace

Article

Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

Insert

Gpat4 (Gpat4 Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(GFP)-PGKpuro2ABFP-W

Plasmid

#200475

Purpose

Lentiviral vector expressing gRNA targeting GFP

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

GFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO-puro-tetON-shRFP

Plasmid

#110940

Purpose

Tet-inducible shRNA targeting RFP

Depositor

William Hahn

Article

Ilic et al Proc Natl Acad Sci U S A. 2017 Apr 25;11

Insert

shRFP

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

H1/TO

Available since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pUltraI-Tet3.0[TAA]

Plasmid

#164580

Purpose

Machinery plasmid containing a single copy of the Mb Tet3.0RS and cognate tRNA for incorporation of Tet3.0 at TAA codons

Depositor

Ryan Mehl

Article

Bednar et al ACS Chem Biol. 2021 Nov 19;16(11):2612-2

Inserts

Mb Tet3.0RS (R2-3)
Mm tRNA[TAA]

Use

Tags

Expression

Bacterial

Mutation

L170G, N311G, C313A

Promoter

ProK and TacI

Available since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX SH3(2)-4R

Plasmid

#112090

Purpose

Bacterial expression plasmid containing a GST tag with 4 repeats of the second SH3 domain from human NCK1.

Depositor

Michael Rosen

Article

Li et al Nature. 2012 Mar 7;483(7389):336-40. doi

Insert

SH3(2)-4R (NCK1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Lac

Available since

Feb. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-DIO ChRmine-oScarlet-KV 2.1-WPRE

Plasmid

#183536

Purpose

Optogenetics

Depositor

Karl Deisseroth

Article

Fan et al Cell. 2023 Feb 2;186(3):543-559.e19. doi

Insert

ChRmine-oScarlet-Kv2.1

Use

AAV

Tags

Expression

Mutation

None

Promoter

CaMKIIa

Available since

Feb. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR-N90-beta-catenin

Plasmid

#31787

Depositor

Xin Chen

Article

Tward et al Proc Natl Acad Sci U S A. 2007 Sep 11;10

Insert

beta-catenin (CTNNB1 Human)

Use

Entry vector

Tags

Myc

Expression

Mutation

Deletion of aa 1-90- Constitutively Active

Promoter

Available since

Oct. 21, 2011

Availability

Academic Institutions and Nonprofits only

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pAAV Syn 2xLyn-ERex-Venus-bPAC(F198Y)

Plasmid

#165491

Purpose

Neuronal expression of PACmn, a photoactivatable adenylyl cyclase derived from bPAC, membrane-anchored, no/reduced dark activity .

Depositor

Thomas Oertner

Article

Yang et al BMC Biol. 2021 Oct 18;19(1):227. doi: 10

Insert

2xLyn-ERex-Venus-bPAC(F198Y)

Use

AAV

Tags

Venus

Expression

Mammalian

Mutation

Promoter

hSyn1

Available since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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R702-X31-566: His6-MBP-tev-G-Hs.PDE6D(2-150)

Plasmid

#159689

Purpose

E. coli protein expression of His6-MBP-tev-G-Hs.PDE6D(2-150)

Depositor

Dominic Esposito

Article

Dharmaiah et al Proc Natl Acad Sci U S A. 2016 Nov 1;113

Insert

His6-MBP-tev-G-Hs.PDE6D(2-150) (PDE6D Human)

Use

Tags

His6-MBP-tev

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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