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pDEST26 Ago2 S387E

Plasmid

#59906

Purpose

Expresses 6xHis-Ago2 (N-termi tag) S387E mutant in mammalian cells

Depositor

Carl Novina

Article

Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

Insert

Argonaute 2 (AGO2 Human)

Use

Tags

6xHis (N-termi tag)

Expression

Mammalian

Mutation

S387E, phospho-dominant

Promoter

CMV

Available since

May 26, 2015

Availability

Academic Institutions and Nonprofits only

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pCDNA3_PKA-RIIα

Plasmid

#168492

Purpose

Mammalian expression of mouse PKA regulatory subunit Iiα, untagged.

Depositor

Haining Zhong

Article

Xiong et al Proc Natl Acad Sci U S A. 2021 Apr 13;11

Insert

mouse PKA regulatory subunit IIα, untagged

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 14, 2021

Availability

Academic Institutions and Nonprofits only

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pmcherry-LCa

Plasmid

#53972

Purpose

Expresses the light chain of clathrin

Depositor

Tom Kirchhausen

Article

Kural et al Cell Rep. 2012 Nov 29;2(5):1111-9. doi:

Insert

clathrin light chain

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 1, 2014

Availability

Academic Institutions and Nonprofits only
pKH3-avian RSK1 K112/464R

Plasmid

#8981

Depositor

John Blenis

Article

Roux et al Mol Cell Biol. 2003 Jul . 23(14):4796-80

Insert

RSK (RPS6KA1 Chicken)

Use

Tags

3x HA

Expression

Mammalian

Mutation

K112R, K464R

Promoter

Available since

Feb. 13, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA6-MRPL50-VC155

Plasmid

#182373

Purpose

mVenus-based mito-riboBiFC vector (Negative control)

Depositor

Chunghun Lim

Article

Lee et al BMC Biol. 2022 Jan 7;20(1):12. doi: 10.1

Insert

Mrpl50 (Mrpl50 Mouse)

Use

Tags

VC155

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA6-MRPS16-VN172

Plasmid

#182375

Purpose

mVenus-based mito-riboBiFC vector (Negative control)

Depositor

Chunghun Lim

Article

Lee et al BMC Biol. 2022 Jan 7;20(1):12. doi: 10.1

Insert

Mrps16 (Mrps16 Mouse)

Use

Tags

VN172

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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pCDH-FLAG-R-Ras2

Plasmid

#102335

Purpose

Human R-Ras2 with FLAG tag for mammalian expression

Depositor

Hening Lin

Article

Zhang et al Elife. 2017 Apr 13;6. pii: e25158. doi:

Insert

RRAS2 (RRAS2 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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psiCheck2-SV40p-3xFLAG-HSVTKp-mCherry

Plasmid

#182377

Purpose

Dual expression construct encoding 3xFLAG and mCherry from separate promoters

Depositor

Chunghun Lim

Article

Lee et al BMC Biol. 2022 Jan 7;20(1):12. doi: 10.1

Inserts

3xFlag
mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

HSV TK promoter and SV40 promoter

Available since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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mCherry-FRBx2

Plasmid

#193571

Purpose

Expresses mCherry tagged with two FRB mutants (T2098L) in mammalian cells.

Depositor

Justin Taraska

Article

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

mCherry

Use

Tags

FRB (T2098L)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA6-MRPS6-VN172

Plasmid

#182374

Purpose

mVenus-based mito-riboBiFC vector

Depositor

Chunghun Lim

Article

Lee et al BMC Biol. 2022 Jan 7;20(1):12. doi: 10.1

Insert

Mrps6 (Mrps6 Mouse)

Use

Tags

VN172

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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pYES2-HTH-HHT2

Plasmid

#86482

Purpose

yeast expression of HHT2 with His10-TEV-HA tag

Depositor

Akash Gunjan

Article

Singh et al Nat Cell Biol. 2009 Aug;11(8):925-33. do

Insert

HHT2 (HHT2 Budding Yeast)

Use

Tags

HIS10-TEV-HA (HTH)

Expression

Yeast

Mutation

Promoter

GAL1

Available since

Jan. 27, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pnEA-NpM-CteIF4G_954-1030_AF

Plasmid

#148700

Purpose

Bacterial Expression of CteIF4G_954-1030

Depositor

Elisa Izaurralde

Article

Gruner et al Nucleic Acids Res. 2018 Jul 27;46(13):68

Insert

CteIF4G_954-1030

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-CteIF4G_996-1033-GB1_AF

Plasmid

#148705

Purpose

Bacterial Expression of CteIF4G_966-1033

Depositor

Elisa Izaurralde

Article

Gruner et al Nucleic Acids Res. 2018 Jul 27;46(13):68

Insert

CteIF4G_966-1033

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3_PKA-RIIα-sREACh

Plasmid

#168489

Purpose

Mammalian expression of mouse PKA regulatory subunit IIα C-terminally tagged by sREACh.

Depositor

Haining Zhong

Article

Xiong et al Proc Natl Acad Sci U S A. 2021 Apr 13;11

Insert

PKA regulatory subunit IIα C-terminally tagged by sREACh

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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IgH P+ VA20

Plasmid

#127249

Purpose

IgH V-region

Depositor

Christine Milcarek

Article

Shell et al J Immunol. 2007 Dec 1;179(11):7663-73.

Insert

Promoter+V-region of IgH of A20 B cell line

Use

Other

Tags

Expression

Mutation

Promoter

IgH

Available since

Aug. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 (+) FLAG-Ago2 S387E

Plasmid

#60653

Purpose

Expresses CMV-FLAG-Ago2 (N-term tag) S387E mutant in mammalian cells

Depositor

Carl Novina

Article

Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

Insert

Argonaute 2 (AGO2 Human)

Use

Tags

CMV-FLAG-Ago2 (N-term tag)

Expression

Mammalian

Mutation

S387E mutant

Promoter

CMV

Available since

Dec. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 (+) FLAG-Ago2 S387A

Plasmid

#60652

Purpose

Expresses CMV-FLAG-Ago2 (N-term tag) S387A mutant in mammalian cells

Depositor

Carl Novina

Article

Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

Insert

Argonaute 2 (AGO2 Human)

Use

Tags

CMV-FLAG-Ago2 (N-term tag)

Expression

Mutation

S387A mutant

Promoter

CMV

Available since

Dec. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pDEST26 Ago2 S387A

Plasmid

#59905

Purpose

Expresses 6xHis-Ago2 (N-termi tag) S387A mutant in mammalian cells

Depositor

Carl Novina

Article

Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

Insert

Argonaute 2 (AGO2 Human)

Use

Tags

6xHis (N-termi tag)

Expression

Mammalian

Mutation

S387A, phospho-null alanine

Promoter

CMV

Available since

May 26, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST26 Ago2 5A

Plasmid

#60651

Purpose

Expresses 6xHis-Ago2 (N-termi tag) mutated five serines to alanines in mammalian cells

Depositor

Carl Novina

Article

Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

Insert

Argonaute 2 (AGO2 Human)

Use

Tags

6xHis (N-termi tag)

Expression

Mammalian

Mutation

mutated five serines to alanines

Promoter

CMV

Available since

May 26, 2015

Availability

Academic Institutions and Nonprofits only

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pLEX307_CR-PDHA2S291D

Plasmid

#115200

Purpose

Lentiviral transduction and expression of CRISPR/Cas9-resistant PDHA2S291D into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')

Depositor

Mohan Babu

Article

Jessulat et al Nucleic Acids Res. 2021 Jul 9. pii: 6318

Insert

CR (CRISPR/Cas9-resistant)-PDHA2S291D (PDHA2 Human)

Use

Lentiviral

Tags

Expression

Mutation

c.321C>T (silent when translated to protein, c…

Promoter

EF1alpha

Available since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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