Addgene

We narrowed to 4,623 results for: codon optimized

Showing: 961 - 980 of 4623 results

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INCTbiosyn-pAct-AtINT9

Plasmid

#127533

Purpose

Plasmid encodes A. thaliana codon optimized Integrase 9.

Depositor

Martin Bonamino

Article

Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

Insert

Integrase 9 coding sequence codon optimized for A. thaliana expression.

Use

Tags

Expression

Plant

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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INCTbiosyn-pAct-AtINT13

Plasmid

#127534

Purpose

Plasmid encodes A. thaliana codon optimized Integrase 13.

Depositor

Martin Bonamino

Article

Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

Insert

Integrase 13 coding sequence codon optimized for A. thaliana expression.

Use

Tags

Expression

Plant

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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INCTbiosyn-pAct-AtINTphiC31

Plasmid

#127535

Purpose

Plasmid encodes A. thaliana codon optimized Integrase phiC31.

Depositor

Martin Bonamino

Article

Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

Insert

Integrase phiC31 coding sequence codon optimized for A. thaliana expression.

Use

Tags

Expression

Plant

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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INCTbiosyn-pAct-AtINTBxb1

Plasmid

#127536

Purpose

Plasmid encodes A. thaliana codon optimized Integrase Bxb1.

Depositor

Martin Bonamino

Article

Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

Insert

Integrase Bxb1 coding sequence codon optimized for A. thaliana expression.

Use

Tags

Expression

Plant

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pIA33

Plasmid

#120803

Purpose

E. coli-C. difficile shuttle vector for CRISPR interference (CRISPRi) in C. difficile; sgRNA targets rfp; Pxyl::dCas9-opt Pgdh::sgRNA-rfp

Depositor

Craig Ellermeier, David S. Weiss

Article

Muh et al J Bacteriol. 2019 Feb 11. pii: JB.00711-

Inserts

deactivated nuclease dCas9, codon-optimized for C. difficile
single guide RNA targeting red fluorescent protein

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Base-pairing region targets red fluorescent prote…

Promoter

Pgdh and Pxyl

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-hAcrVA1-NLS(sv40) (BPK5050)

Plasmid

#115136

Purpose

CMV-T7 promoter expression plasmid for human codon optimized AcrVA1 anti-CRISPR protein with C-terminal NLS

Depositor

Benjamin Kleinstiver, Keith Joung, Joseph Bondy-Denomy

Article

Marino et al Science. 2018 Sep 6. pii: science.aau517

Insert

human codon optimized AcrVA1 anti-CRISPR protein

Use

Tags

NLS(SV40)

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-hAcrVA2.1-NLS(sv40) (BPK5059)

Plasmid

#115137

Purpose

CMV-T7 promoter expression plasmid for human codon optimized AcrVA2.1 anti-CRISPR protein with C-terminal NLS

Depositor

Benjamin Kleinstiver, Keith Joung, Joseph Bondy-Denomy

Article

Marino et al Science. 2018 Sep 6. pii: science.aau517

Insert

human codon optimized AcrVA2.1 anti-CRISPR protein

Use

Tags

NLS(SV40)

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-hAcrVA2-NLS(sv40) (AAS2283)

Plasmid

#115138

Purpose

CMV-T7 promoter expression plasmid for human codon optimized AcrVA2 anti-CRISPR protein with C-terminal NLS

Depositor

Benjamin Kleinstiver, Keith Joung, Joseph Bondy-Denomy

Article

Marino et al Science. 2018 Sep 6. pii: science.aau517

Insert

human codon optimized AcrVA2 anti-CRISPR protein

Use

Tags

NLS(SV40)

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-hAcrVA3.1-NLS(sv40) (RTW2624)

Plasmid

#115139

Purpose

CMV-T7 promoter expression plasmid for human codon optimized AcrVA3.1 anti-CRISPR protein with C-terminal NLS

Depositor

Benjamin Kleinstiver, Keith Joung, Joseph Bondy-Denomy

Article

Marino et al Science. 2018 Sep 6. pii: science.aau517

Insert

human codon optimized AcrVA3.1 anti-CRISPR protein

Use

Tags

NLS(SV40)

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-hAcrVA3-NLS(sv40) (BPK5077)

Plasmid

#115140

Purpose

CMV-T7 promoter expression plasmid for human codon optimized AcrVA3 anti-CRISPR protein with C-terminal NLS

Depositor

Benjamin Kleinstiver, Keith Joung, Joseph Bondy-Denomy

Article

Marino et al Science. 2018 Sep 6. pii: science.aau517

Insert

human codon optimized AcrVA3 anti-CRISPR protein

Use

Tags

NLS(SV40)

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-hOrf2mor-NLS(sv40) (BPK5095)

Plasmid

#115141

Purpose

CMV-T7 promoter expression plasmid for human codon optimized Orf2mor anti-CRISPR protein with C-terminal NLS

Depositor

Benjamin Kleinstiver, Keith Joung, Joseph Bondy-Denomy

Article

Marino et al Science. 2018 Sep 6. pii: science.aau517

Insert

human codon optimized Orf2mor anti-CRISPR protein

Use

Tags

NLS(SV40)

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pCAG-hMb3Cas12a-NLS(nucleoplasmin)-3xHA (RTW2500)

Plasmid

#115142

Purpose

CAG promoter expression plasmid for human codon optimized Mb3Cas12a nuclease with C-terminal NLS and HA tag

Depositor

Benjamin Kleinstiver, Keith Joung, Joseph Bondy-Denomy

Article

Marino et al Science. 2018 Sep 6. pii: science.aau517

Insert

human codon optimized Mb3Cas12a

Use

Tags

NLS(nucleoplasmin) and 3xHA

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pHDM-Hgpm2

Plasmid

#164441

Purpose

Encodes codon-optimized HIV gagpol for pseudovirus production

Depositor

Alejandro Balazs

Article

Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

Insert

Codon Optimized HIV-1 GagPol

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pCMV10-3xFLAG-prs-hMIGA2 (170-593)

Plasmid

#192833

Purpose

Express codon-optimized human MIGA2 fragment 170-593 in Expi293 cells

Depositor

Karin Reinisch

Article

Hong et al J Cell Biol. 2022 Dec 5;221(12):e2022070

Insert

Codon-optimized human Mitoguardin 2 (MIGA2 Human)

Use

Tags

3xFLAG-PreScission cleavage site

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pET29-hMIGA2(170-593)-6xhis

Plasmid

#192834

Purpose

Express codon-optimized human MIGA2 fragment 170-593 with a C-terminal 6xhis tag (lipid transfer construct) in bacteria

Depositor

Karin Reinisch

Article

Hong et al J Cell Biol. 2022 Dec 5;221(12):e2022070

Insert

Codon-optimized human Mitoguardin 2 (MIGA2 Human)

Use

Tags

6xhis

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pET29-hMIGA2(306-593)-6xhis

Plasmid

#192837

Purpose

Express codon-optimized human MIGA2 fragment 306-593 with a C-terminal 6xhis tag (lipid transfer construct) in bacteria

Depositor

Karin Reinisch

Article

Hong et al J Cell Biol. 2022 Dec 5;221(12):e2022070

Insert

Codon-optimized human Mitoguardin 2 (MIGA2 Human)

Use

Tags

6xhis

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pLVX-hMIGA2-DsRed

Plasmid

#192866

Purpose

Express codon-optimized human MIGA2 full length with a C-terminal DsRed tag in mammalian cells

Depositor

Karin Reinisch

Article

Hong et al J Cell Biol. 2022 Dec 5;221(12):e2022070

Insert

Codon-optimized human Mitoguardin 2 (MIGA2 Human)

Use

Tags

DsRed

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pGK-hMIGA2-DsRed

Plasmid

#192867

Purpose

Express codon-optimized human MIGA2 full length with a C-terminal DsRed tag in mammalian cells

Depositor

Karin Reinisch

Article

Hong et al J Cell Biol. 2022 Dec 5;221(12):e2022070

Insert

Codon-optimized human Mitoguardin 2 (MIGA2 Human)

Use

Tags

DsRed

Expression

Mammalian

Mutation

Promoter

PGK

Availability

Academic Institutions and Nonprofits only

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pEF1a-hMLH1NTD-NLS

Plasmid

#174826

Purpose

Mammalian expression of human MLH1NTD-NLS (codon optimized)

Depositor

David Liu

Article

Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

Insert

human MLH1_NTD-NLS (codon optimized)

Use

Tags

SV40 bpNLS

Expression

Mammalian

Mutation

truncation of residues 1-335

Promoter

EF1a

Availability

Academic Institutions and Nonprofits only

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pCMVd1-NLS-GAL4-iLight-VP16-T2A-mTagBFP2

Plasmid

#170273

Purpose

NLS-Gal4(1-65)-iLight(human codon optimized)-VP16-T2A-mTagBFP2 construct expression in mammalian cells.

Depositor

Vladislav Verkhusha

Article

Kaberniuk et al Nat Commun. 2021 Jun 23;12(1):3859. doi:

Insert

NLS-Gal4(1-65)-iLight(human codon optimized)-VP16-T2A-mTagBFP2

Use

Tags

Expression

Mammalian

Mutation

See Comments

Promoter

CMVd1

Availability

Academic Institutions and Nonprofits only

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