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Plasmid#11427DepositorInsertItch (Itch Mouse)
UseTagsmycExpressionMammalianMutationn/aPromoterAvailable sinceApril 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
pOPINB-OTULIN (full-length, aa 1-352)
Plasmid#61464PurposeExpresses human OTULIN (full-length) in E. coli.DepositorInsertOTULIN (OTULIN Human)
UseTagsHis6-3CExpressionBacterialMutationPromoterT7Available sinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX-KG-Itch/2793
Plasmid#11434DepositorInsertItch (Itch Mouse)
UseTagsGSTExpressionBacterialMutationn/aPromoterAvailable sinceApril 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
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1432 pcDNA3 flag MAGI1c
Plasmid#10714DepositorInsertMAGI-1 (Magi1 Mouse)
UseTagsflagExpressionMammalianMutationPromoterAvailable sinceSept. 28, 2005AvailabilityAcademic Institutions and Nonprofits only -
Venus-Noxa-pEGFP-C1
Plasmid#166738PurposeExpress Venus fused to the N-terminus of the Bcl-2 family protein, NoxaDepositorInsertNoxa (PMAIP1 Human)
UseTagsVenusExpressionMammalianMutationPromoterCMVAvailable sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
ALIX(1-702)-mNeonGreen
Plasmid#191193PurposeTruncated form of ALIX that lacks C-terminal proline-rich domain; C-terminally tagged with mNeonGreen.DepositorInsertALIX (1-702) (PDCD6IP Human, Synthetic)
UseTags6xHIS and mNeonGreenExpressionMammalianMutationDeletion of ALIX C-terminal PRD.PromoterCMVAvailable sinceOct. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
GB1-PRD(800-868)-strep
Plasmid#180023PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(800-868)-Strep.DepositorInsertGB1-His6-TEV-ALIX(800-868)-Strep (PDCD6IP Human)
UseTagsGB1-His6-TEV and Strep tag (WSHPQFEK)ExpressionBacterialMutationPromoterAvailable sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
GB1-ALIX(P801G)
Plasmid#180025PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(1-868, P801G). P801G mutation facilitates expression in E. coli.DepositorInsertGB1-His6-TEV-ALIX(1-868, P801G) (PDCD6IP Human)
UseTagsGB1-His6-TEVExpressionBacterialMutationP801GPromoterAvailable sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHisAlix deltaPRD
Plasmid#42577DepositorInsertdelta-PRD ALIX (residues 1-702) (PDCD6IP Human)
UseTagsHIS-TEVExpressionBacterialMutationsilent mutation A1617G (numbering in ALIX gene)PromoterAvailable sinceDec. 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_NOSTRIN-1/1-SV1/2
Plasmid#91323PurposeProtein expression and purification of human SH3 domain construct NOSTRIN-1/1-SV1/2DepositorInsertNOSTRIN-1/1-SV1/2 (NOSTRIN Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
ALIX(P801G)-mNeonGreen
Plasmid#191192PurposeFull-length ALIX with P801G mutation in proline-rich domain; C-terminally tagged with mNeonGreen.DepositorInsertALIX (P801G) (PDCD6IP Human, Synthetic)
UseTags6xHIS and mNeonGreenExpressionMammalianMutationPro 801 mutated to GlyPromoterCMVAvailable sinceOct. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pCINeo-myc-Itch (C822S)/2860
Plasmid#11428DepositorInsertItch (Itch Mouse)
UseTagsmycExpressionMammalianMutationcysteine at 822 mutated to serinePromoterAvailable sinceApril 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
GB1-PRD(703-868, P801G)-Strep
Plasmid#164444PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-868, P801G)-Strep.DepositorInsertGB1-His6-TEV-ALIX(703-868, P801G)-Strep (PDCD6IP Human)
UseTagsGB1-His6-TEV and Strep tag (WSHPQFEK)ExpressionBacterialMutationProline 801 to GlycinePromoterAvailable sinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMV10 lenti pEF-rTetR(SE-G72P)-3XFLAG-WWP2(WW)-T2A-mCherry-BSD-WPRE
Plasmid#188756PurposeLentiviral backbone containing WWP2 WW domain as a fusion with rTetR(SE-G72P)-3XFLAGDepositorArticleInsertWWP2 WW (WWP2 Human)
UseLentiviralTagsrTetR(SE-G72P)-3xFLAGExpressionMammalianMutationPromoterpEF1aAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
GB1-PRD(703-868, P801G)
Plasmid#180029PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-868, P801G). P801G mutation facilitates expression in E. coli.DepositorInsertGB1-His6-TEV-ALIX(703-868, P801G) (PDCD6IP Human)
UseTagsGB1-His6-TEVExpressionBacterialMutationP801GPromoterAvailable sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_HCK-1/1
Plasmid#91273PurposeProtein expression and purification of human SH3 domain construct HCK-1/1DepositorInsertHCK-1/1 (HCK Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
TFORF1640
Plasmid#142812PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertPSIP1 (PSIP1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only