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Plasmid#1186DepositorInserthtt 103Q (HTT Human)
UseTagsGFPExpressionYeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only
These results may also be relevant.
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pEM705 Gso (Ser)
Plasmid#109353PurposeGsX chimera with Gαo C-terminus (Pertussis toxin insensitive mutant)DepositorInsertGso chimera
UseTagsNoneExpressionMammalianMutationChanged Cysteine at position 391 to Serine (Pertu…PromoterCAGAvailable sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEM705 Gsi (Ser)
Plasmid#109352PurposeGsX chimera with Gαi C-terminus (Pertussis toxin insensitive mutant)DepositorInsertGsi chimera
UseTagsNoneExpressionMammalianMutationChanged Cys at position 391 to Serine (Pertussis …PromoterCAGAvailable sinceMay 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTC217
Plasmid#70018PurposeCas9/sgRNA targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon based on Bean Yellow Dwarf Virus; sgRNA= 1bDepositorInsertNuclease (Cas9/sgRNA) + Donor + GVR
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMarch 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pI2-MTU-DO-G418
Plasmid#160018PurposeIntegrative vector for K. marxianus, targeting integration site I2 described in (Rajkumar et al.,2019). G418 selection.DepositorTypeEmpty backboneUseKluyveromyces marxianusTagsExpressionYeastMutationPromotern/aAvailable sinceNov. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pI4-MTU-DO-G418
Plasmid#160215PurposeIntegrative vector for K. marxianus, targeting integration site I4 described in (Rajkumar et al.,2019). G418 selection.DepositorTypeEmpty backboneUseKluyveromyces marxianusTagsExpressionYeastMutationPromotern/aAvailable sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pR26 GFP Dest
Plasmid#74283PurposeGene targeting vector for the mouse Rosa26 locus, including a loxP flanked stop cassette and GFP, for gateway cloningDepositorInsertRosa26 5-homology region
UseTagsExpressionMutationPromoterAvailable sinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFBD-SpyTag-noro-VLP
Plasmid#165989PurposeExpresses norovirus-like particles with SpyTags presented on their surface for convenient decoration of SpyCatcher fusion proteinsDepositorInsertSpyTag-noro-VLP (ORF2 norovirus, Synthetic)
UseTagsSpyTagExpressionInsectMutationPromoterPolyhedrinAvailable sinceMarch 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pSF464
Plasmid#167672PurposeIntermediate vector for four gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for GatewayDepositorInsertattL1-ccdB-attL2
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZmUbi-5'UTR
Plasmid#154057PurposeLevel 0 Golden Gate vector, containing the ZmUbi promoter and 5' UTR with Level 0.5-compatible overhangs (Wheat construct)DepositorInsertPromoter and 5' UTR Ubiquitin (Zea mays)
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterN/AAvailable sinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPB-R1R2_EF1ap300dCas9VP64_T2A_MS2p65HSF1-IRESbsdpA
Plasmid#113343PurposeExpresses p300-dCas9-VP64 and MS2-p65-HSF1 fusion proteinsDepositorInsertp300core-dCas9-VP64-T2A-MS2-p65-HSF1
UsePiggybacTagsExpressionMutationPromoterAvailable sinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFREE_Cm
Plasmid#92052PurposeAllows inducible expression of gRNAs and Cas9 for plasmid curing.DepositorInsertsCas9
gRNA array
UseCRISPRTagsExpressionMutationPromoterpRhamBAD and ptetAvailable sinceAug. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
HA-tag
Plasmid#55182PurposeHA-tagDepositorInsertHA tag
UseSynthetic Biology; Bacillus biobrick boxTagsExpressionMutationPromoterAvailable sinceJuly 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
SPI1_pet28a
Plasmid#131653PurposeBacterial expression of ETS gene SPI1DepositorInsertSPI1 (SPI1 Human)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAC1430
Plasmid#127981PurposeAMA1 plasmid with Aspergillus optimized Cpf1, pyrG selection marker, and USER cassette (PacI/Nt.BbvCI)DepositorInsertCpf1
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialMutationcodon optimized for Aspergillus nidulansPromoterAspergillus nidulans tef1 promoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGS-21a-PcAad1
Plasmid#163039Purposeexpression of aryl-alcohol dehydrogenase from the white-rot fungus Phanerochaete chrysosporiumDepositorInsertaryl-alcohol dehydrogenase
UseTagsGST and His6ExpressionBacterialMutationPromoterAvailable sinceJan. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZmUBQ:LUC
Plasmid#131860PurposeTransient expression of firefly luciferase from the Zea mays ubiquitin promotorDepositorInsertluciferase
UseTagsExpressionPlantMutationPromoterZmUBQAvailable sinceOct. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZmUBQ:GW
Plasmid#132358PurposeExpression of CDS from the Zea mays ubiquitin promotorDepositorTypeEmpty backboneUseTagsYFPExpressionPlantMutationPromoterpZmUBQAvailable sinceOct. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Cas9-162A
Plasmid#84918Purposefor in vitro transcription of Cas9 with polyA tail encodedDepositorInsertCas9
UseTagsFlag, NLS, and polyA tailExpressionMammalianMutationPromoterCAGAvailable sinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSF278
Plasmid#167673PurposeIntermediate vector for three gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for GatewayDepositorInsertattL1-ccdB-attL2
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only