1. p416 103Q GAL

   Plasmid

   #1186

   Depositor

   Susan Lindquist

   Article

   Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

   Insert

   htt 103Q (HTT Human)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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These results may also be relevant.

1. pUPD-PGK1 (01-11)

   Plasmid

   #101689

   Purpose

   pPGK1 in domestication vector

   Depositor

   Elena Caro Bernat

   Article

   Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

   Insert

   GGAG-[promoter of S. cerevisiae YCR012W (982bp)]-CCAT
   

   Use

   Yeast expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pFH85

   Plasmid

   #128198

   Purpose

   classic sgRNA backbone for SpCas9, combine with TaU3 promoter module (pFH33)

   Depositor

   Vladimir Nekrasov

   Article

   Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

   Insert

   classic sgRNA backbone for SpCas9, combine with TaU3 promoter module (pFH33)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pPB-R1R2-EM7neoPheS

   Plasmid

   #112923

   Purpose

   piggyBac Transposon vector with a Gateway destination cassette (neoPheS selection)

   Depositor

   Kosuke Yusa

   Article

   Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

   Insert

   neoPheS
   

   Use

   Piggybac transposon gateway destination vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAC575

   Plasmid

   #127976

   Purpose

   AMA1 plasmid with ble selection marker, and USER cassette (PacI/Nt.BbvCI)

   Depositor

   Uffe Mortensen

   Article

   Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.

   Insert

   ble (bleomycin resistance marker)
   

   Use

   Fungal expression

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Aspergillus nidulans trpC promoter

   Available since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pTC208

   Plasmid

   #70017

   Purpose

   TALENs targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon based onTomato Yellow Leaf Curl Virus

   Depositor

   Daniel Voytas

   Article

   Cermak et al Genome Biol. 2015 Nov 6;16(1):232. doi:

   Insert

   Nuclease (TALENs) + Donor + GVR
   

   Use

   TALEN

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Jan. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pI4-MTU-DO-HIS

   Plasmid

   #160026

   Purpose

   Integrative vector for K. marxianus, targeting integration site I4 described in (Rajkumar et al.,2019). Histidine selection.

   Depositor

   John Morrissey

   Article

   Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

   Type

   Empty backbone

   Use

   Kluyveromyces marxianus

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   n/a

   Available since

   March 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pI2-MTU-DO-HPH

   Plasmid

   #160019

   Purpose

   Integrative vector for K. marxianus, targeting integration site I2 described in (Rajkumar et al.,2019). Hygromycin selection.

   Depositor

   John Morrissey

   Article

   Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

   Type

   Empty backbone

   Use

   Kluyveromyces marxianus

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   n/a

   Available since

   Nov. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pI2-MTU-DO-LEU

   Plasmid

   #160017

   Purpose

   Integrative vector for K. marxianus, targeting integration site I2 described in (Rajkumar et al.,2019). Leucine selection.

   Depositor

   John Morrissey

   Article

   Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

   Type

   Empty backbone

   Use

   Kluyveromyces marxianus

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   n/a

   Available since

   Nov. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pFH42

   Plasmid

   #125881

   Purpose

   Level0 Cestrum Yellow Leaf Curling Virus CmYLCV Pol II promoter

   Depositor

   Vladimir Nekrasov

   Article

   Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

   Insert

   Level0 Cestrum Yellow Leaf Curling Virus CmYLCV Pol II promoter
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFH86

    Plasmid

    #128199

    Purpose

    improved sgRNA backbone for SpCas9, combine with TaU3 promoter module (pFH33)

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    improved sgRNA backbone for SpCas9, combine with TaU3 promoter module (pFH33)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET-Cdc12-Cdc11

    Plasmid

    #131162

    Purpose

    Expression of Septin rods

    Depositor

    Nils Johnsson

    Article

    Renz et al BMC Biotechnol. 2013 Jul 26;13:60. doi:

    Insert

    6his-Cdc12 + Cdc11
    

    Use

    Bicistronic

    Tags

    Cdc11: S-Tag and Cdc12: 6his

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAc-Cdc3-Cdc10

    Plasmid

    #131160

    Purpose

    Expression of Septin rods

    Depositor

    Nils Johnsson

    Article

    Renz et al BMC Biotechnol. 2013 Jul 26;13:60. doi:

    Insert

    Cdc3 + Cdc10
    

    Use

    Bicistronic

    Tags

    Cdc10: S Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pUC19-U6-crRNA1

    Plasmid

    #123639

    Purpose

    Mammalian expression, Genome editing, gRNA scaffold

    Depositor

    Wei Li

    Article

    Teng et al Genome Biol. 2019 Feb 5;20(1):15. doi: 1

    Insert

    crRNA scaffold 1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pUPD-G418R (01-21)

    Plasmid

    #101703

    Purpose

    G418R in domestication vector

    Depositor

    Elena Caro Bernat

    Article

    Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

    Insert

    GGAG-[G418R (loxP-PAgTEF-kanMX-TAgTEF-loxP)]-CGCT
    

    Use

    Yeast expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFH88

    Plasmid

    #128201

    Purpose

    sgRNA backbone for SaCas9, combine with TaU3 promoter module (pFH33)

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    sgRNA backbone for SaCas9, combine with TaU3 promoter module (pFH33)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pUPD-Su9MTS (12)

    Plasmid

    #101691

    Purpose

    Su9MTS in domestication vector

    Depositor

    Elena Caro Bernat

    Article

    Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

    Insert

    CCAT-[MTS from Neurospora crassa ATP synthase subunit 9 (OR74A)]-AATG
    

    Use

    Yeast expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pUASp YFP Rho1 DN

    Plasmid

    #52231

    Purpose

    Drosophila expression of YFP-Rho1 dominant negative mutant

    Depositor

    Matthew Scott

    Insert

    Rho1 (Rho1 Fly)
    

    Use

    Drosophila

    Tags

    YFP

    Expression

    Insect

    Mutation

    T19N; DN mutation

    Promoter

    GAL4

    Available since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pCAG-sfGFP-GSAx9-iCre-ERT2

    Plasmid

    #125748

    Purpose

    sfGFP and iCreERT2 fused by GSAx9 linker

    Depositor

    Mario Capecchi

    Article

    Nam et al Neural Dev. 2020 Mar 17;15(1):3. doi: 10

    Insert

    sfGFP-GSAx9-iCre-ERT2
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. p426-SNR52p-gRNA.clr-2.Y-SUP4t

    Plasmid

    #89692

    Purpose

    gRNA for clr-2 locus

    Depositor

    Christian Hong

    Article

    Matsu-Ura et al Fungal Biol Biotechnol. 2015 Jul 15;2:4.

    Insert

    gRNA for clr-2 locus
    

    Use

    CRISPR; Grna

    Tags

    No

    Expression

    Mutation

    Promoter

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAG-TetR-RNF169UBD

    Plasmid

    #119011

    Purpose

    Mammalian expression of a fusion protein of single chain Tet repressor with the human RNF169 Ubiquitin binding domain and BFP expression

    Depositor

    Ralf Kuehn

    Article

    Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

    Insert

    TetR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge