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Plasmid#202745Purposeexpresses recruitable fluorescent protein-tagged enzymeDepositorInsertmCherry:FKBP1A(3-108) : [GGSA]4GG :PIP4K2A (33-406) (PIP4K2A Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
LentiU6-hMYOD1 gRNA_1-MS2-Puro
Plasmid#192673PurposeLentiviral expression of sgRNA targeting hMYOD1 promoter to activate human MYOD1 transcriptionDepositorInsertHuman MYOD1 activating gRNA #1 (MYOD1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
LentiU6-hMYOD1 gRNA_1-MS2-Puro
Plasmid#192673PurposeLentiviral expression of sgRNA targeting hMYOD1 promoter to activate human MYOD1 transcriptionDepositorInsertHuman MYOD1 activating gRNA #1 (MYOD1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
OTUB1/pRetroX-IRES-DsRed#10
Plasmid#180279PurposePlasmid encoding the human protease OTUB1 without a tag. Polycistronic red fluorescent retroviral vector (unmodified from TAKARA BIO).DepositorInserthuman Otubain-1 (OTUB1 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
OTUB1/pRetroX-IRES-DsRed#10
Plasmid#180279PurposePlasmid encoding the human protease OTUB1 without a tag. Polycistronic red fluorescent retroviral vector (unmodified from TAKARA BIO).DepositorInserthuman Otubain-1 (OTUB1 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
OA-1010Av2
Plasmid#176082PurposepBac-10xUAS-L596_g9608-p10-UTR-Opie2-dsRedDepositorInsertL596_g9608.t1
UseTags3xHAExpressionBacterialMutationPromoterAvailable sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
OA-1010Bv2
Plasmid#176083PurposepBac-10xUAS-L596_g25050-p10-UTR-Opie2-dsRedDepositorInsertL596_g25050.t1
UseTags3xHAExpressionBacterialMutationPromoterAvailable sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
OA-1010Av2
Plasmid#176082PurposepBac-10xUAS-L596_g9608-p10-UTR-Opie2-dsRedDepositorInsertL596_g9608.t1
UseTags3xHAExpressionBacterialMutationPromoterAvailable sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
OA-1010Bv2
Plasmid#176083PurposepBac-10xUAS-L596_g25050-p10-UTR-Opie2-dsRedDepositorInsertL596_g25050.t1
UseTags3xHAExpressionBacterialMutationPromoterAvailable sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T3-Stx3-L77A/L80A
Plasmid#100273PurposeExpresses GST-Stx3-L77A/L80A in bacteria.DepositorInsertStx3-1-265-L77A/L80A (Stx3 Rat)
UseTagsGSTExpressionBacterialMutationNo transmembrane domain; residues 4-264 present, …PromoterTacAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T3-Stx3-L77A/L80A
Plasmid#100273PurposeExpresses GST-Stx3-L77A/L80A in bacteria.DepositorInsertStx3-1-265-L77A/L80A (Stx3 Rat)
UseTagsGSTExpressionBacterialMutationNo transmembrane domain; residues 4-264 present, …PromoterTacAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T3-Stx3-E78A/E83A
Plasmid#100272PurposeExpresses GST-Stx3-E78A/E83A in bacteria.DepositorInsertStx3-1-265-E78A/E83A (Stx3 Rat)
UseTagsGSTExpressionBacterialMutationNo transmembrane domain; residues 4-264 present, …PromoterTacAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T3-Stx3-E78A/E83A
Plasmid#100272PurposeExpresses GST-Stx3-E78A/E83A in bacteria.DepositorInsertStx3-1-265-E78A/E83A (Stx3 Rat)
UseTagsGSTExpressionBacterialMutationNo transmembrane domain; residues 4-264 present, …PromoterTacAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T3-Stx3-LE165/166AA
Plasmid#100271PurposeExpresses GST-tagged open-conformation mutant of Stx3 in bacteria.DepositorInsertStx3-1-265-LE165/166AA (Stx3 Rat)
UseTagsGSTExpressionBacterialMutationNo transmembrane domain; residues 4-264 present, …PromotertacAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T3-Stx3-LE165/166AA
Plasmid#100271PurposeExpresses GST-tagged open-conformation mutant of Stx3 in bacteria.DepositorInsertStx3-1-265-LE165/166AA (Stx3 Rat)
UseTagsGSTExpressionBacterialMutationNo transmembrane domain; residues 4-264 present, …PromotertacAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDB367
Plasmid#110277PurposegHEEE_02 with a N-terminal 10-His DsbC TEV-peptide fusionDepositorInsertgHEEE_02
UseTags10-His, Tev recognition peptide, and mature DsbC …ExpressionBacterialMutationPromoterT7Available sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDB367
Plasmid#110277PurposegHEEE_02 with a N-terminal 10-His DsbC TEV-peptide fusionDepositorInsertgHEEE_02
UseTags10-His, Tev recognition peptide, and mature DsbC …ExpressionBacterialMutationPromoterT7Available sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pQCXIH-Flag Smad3 AAGY
Plasmid#27027DepositorInsertSmad3 (SMAD3 Human)
UseRetroviralTagsFlagExpressionMammalianMutationP181A P182APromoterAvailable sinceJan. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pQCXIH-Flag Smad3 AAGY
Plasmid#27027DepositorInsertSmad3 (SMAD3 Human)
UseRetroviralTagsFlagExpressionMammalianMutationP181A P182APromoterAvailable sinceJan. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Anillin
Plasmid#68027PurposeFull length Anillin rendered RNAi-resistant) in mammalian expression vector pEGFP-C1DepositorInsertAnillin (ANLN Human)
UseTagseGFPExpressionMammalianMutationsilent mutations at 798 5'-TGCC TCTTTGAATAAA…PromoterCMVAvailable sinceSept. 11, 2015AvailabilityAcademic Institutions and Nonprofits only