Addgene

We narrowed to 11,165 results for: cel.2

Showing: 9761 - 9780 of 11165 results

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pCMV(WT)-mouse-full-length-CHMP3-HA

Plasmid

#154175

Purpose

expresses mouse CHMP3 in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

Insert

CHMP3 (Chmp3 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pEF1α-mouse-CHMP3(147)-HA

Plasmid

#154177

Purpose

expresses mouse CHMP3(147) in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

Insert

CHMP3 (Chmp3 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

C-terminal truncation of CHMP3 at amino acid 147

Promoter

EF1alpha

Available since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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HA-mRIN2 dRA/PINCO

Plasmid

#108938

Purpose

Retroviral expression of mRIN2 dRA

Depositor

Guido Serini

Article

Sandri et al Cell Res. 2012 Oct;22(10):1479-501. doi:

Insert

Ras and Rab interactor 2 (Rin2 Mouse)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Deletion of RA domain (aa 751-836)

Promoter

LTR

Available since

June 25, 2018

Availability

Academic Institutions and Nonprofits only

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HA-mRIN2 dRHVPS9/PINCO

Plasmid

#108939

Purpose

Retroviral expression of mRIN2 dRHVPS9

Depositor

Guido Serini

Article

Sandri et al Cell Res. 2012 Oct;22(10):1479-501. doi:

Insert

Ras and Rab interactor 2 (Rin2 Mouse)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Deletion of RHVPS9 domain (aa 455-738)

Promoter

LTR

Available since

June 25, 2018

Availability

Academic Institutions and Nonprofits only

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pMIR-Report-SPRED1-3'UTR (containing miR-21 sites)

Plasmid

#62579

Purpose

Translational Luciferase Reporter encoding a fragment of the 3'UTR of SPRED1 containing two miR-21 binding sites (sites 1 and 2)

Depositor

Michael Ruppert

Article

Sharma et al Mol Cell Biol. 2014 Nov 15;34(22):4143-6

Insert

SPRED1 (SPRED1 Human)

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

June 4, 2015

Availability

Academic Institutions and Nonprofits only
pFUW-tetO-3xFLAG-GATA2

Plasmid

#125600

Purpose

doxycycline-inducible expression of human GATA2 (3xFLAG tagged) in mammalian cells

Depositor

Filipe Pereira

Article

Gomes et al Cell Rep. 2018 Dec 4;25(10):2821-2835.e7

Insert

GATA binding protein 2 (GATA2 Human)

Use

Lentiviral

Tags

3xFLAG tag

Expression

Mammalian

Mutation

Promoter

TRE-mCMV

Available since

July 9, 2019

Availability

Academic Institutions and Nonprofits only

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pFRT-TO-RPB1-His WT CRres si2,4R

Plasmid

#139404

Purpose

Dox-inducible, wt C-terminal His tagged RPB1, resistant to siRNAs 2 & 4, for generation of TRex flp In cells.

Depositor

Jesper Svejstrup

Article

Tufegdzic Vidakovic et al Cell. 2020 Mar 5. pii: S0092-8674(20)301

Insert

RPB1 (POLR2A Human)

Use

Tags

6xHis

Expression

Mammalian

Mutation

Promoter

pCMV, TRex

Available since

April 21, 2020

Availability

Academic Institutions and Nonprofits only

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Gfp-zf-vasa

Plasmid

#167323

Purpose

Plasmid for synthesis of GFP chimeric RNA that contained vasa-3'UTRs of zebrafish by in vitro transcription.

Depositor

Goro Yoshizaki

Article

Yoshizaki et al Biol Reprod. 2005 Jul;73(1):88-93. doi:

Insert

zf vasa 3'UTRs (ddx4 Zebrafish)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 28, 2021

Availability

Academic Institutions and Nonprofits only

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WT1 KI gRNA1

Plasmid

#92312

Purpose

CRISPR-GFP-gRNA for cutting WT1

Depositor

Sean Palecek

Article

Bao et al Nat Biomed Eng. 2016;1. pii: 0003. doi:

Insert

WT1 (WT1 Human)

Use

Tags

CRISPR GFP

Expression

Mammalian

Mutation

Promoter

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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WT1 KI gRNA2

Plasmid

#92313

Purpose

CRISPR-GFP-gRNA for cutting WT1

Depositor

Sean Palecek

Article

Bao et al Nat Biomed Eng. 2016;1. pii: 0003. doi:

Insert

WT1 (WT1 Human)

Use

Tags

CRISPR GFP

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pFRT-TO-RPB1-His K1268R CRres si2,4R

Plasmid

#139405

Purpose

Dox-inducible, K1268R C-terminal His tagged RPB1, resistant to siRNAs 2 & 4, for generation of TRex flp In cells.

Depositor

Jesper Svejstrup

Article

Tufegdzic Vidakovic et al Cell. 2020 Mar 5. pii: S0092-8674(20)301

Insert

RPB1 (POLR2A Human)

Use

Tags

6xHis

Expression

Mammalian

Mutation

K1268R

Promoter

Available since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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pFRT-TO-RPB1-His K1278R CRres si2,4R

Plasmid

#139406

Purpose

Dox-inducible, K1278R C-terminal His tagged RPB1, resistant to siRNAs 2 & 4, for generation of TRex flp In cells.

Depositor

Jesper Svejstrup

Article

Tufegdzic Vidakovic et al Cell. 2020 Mar 5. pii: S0092-8674(20)301

Insert

RPB1 (POLR2A Human)

Use

Tags

6xHis

Expression

Mammalian

Mutation

K1278R

Promoter

Available since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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pLEX-HA-birA*-K-Ras(wildtype)-IRES-Puro

Plasmid

#120561

Purpose

Expresses HA-birA*-K-Ras wildtype fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

KRAS4B (KRAS Human)

Use

Lentiviral

Tags

HA-birA*

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pLEX-HA-birA*-K-Ras(G12D)-IRES-Puro

Plasmid

#120562

Purpose

Expresses HA-birA*-K-Ras G12D fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

KRAS4B (KRAS Human)

Use

Lentiviral

Tags

HA-birA*

Expression

Mammalian

Mutation

G12D

Promoter

CMV

Available since

March 18, 2019

Availability

Academic Institutions and Nonprofits only

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pLEX-FKBP-Kras-Blasticidin

Plasmid

#120714

Purpose

Expresses membrane-targeted recruiter protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

KRAS4B (KRAS Human)

Use

Lentiviral

Tags

FKBP and mCherry

Expression

Mammalian

Mutation

amino acids 158-188

Promoter

CMV

Available since

Jan. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pFRT-TO-RPB1-His CRres K619,627,642R si2,4R

Plasmid

#139409

Purpose

Dox-inducible, K619, 627, 642R C-terminal His tagged RPB1, resistant to siRNAs 2 & 4, for generation of TRex flp In cells.

Depositor

Jesper Svejstrup

Article

Tufegdzic Vidakovic et al Cell. 2020 Mar 5. pii: S0092-8674(20)301

Insert

RPB1 (POLR2A Human)

Use

Tags

6xHis

Expression

Mammalian

Mutation

K619, 627, 642R

Promoter

Available since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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pFRT-TO-RPB1-His K751,758,767R CRres si2,4R

Plasmid

#139408

Purpose

Dox-inducible, K751, 758, 767R C-terminal His tagged RPB1, resistant to siRNAs 2 & 4, for generation of TRex flp In cells.

Depositor

Jesper Svejstrup

Article

Tufegdzic Vidakovic et al Cell. 2020 Mar 5. pii: S0092-8674(20)301

Insert

RPB1 (POLR2A Human)

Use

Tags

6xHis

Expression

Mammalian

Mutation

K751, 758, 767R

Promoter

Available since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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pFRT-TO-RPB1-His K710,719R CRres si2,4R

Plasmid

#139407

Purpose

Dox-inducible, K710, 719R C-terminal His tagged RPB1, resistant to siRNAs 2 & 4, for generation of TRex flp In cells.

Depositor

Jesper Svejstrup

Article

Tufegdzic Vidakovic et al Cell. 2020 Mar 5. pii: S0092-8674(20)301

Insert

RPB1 (POLR2A Human)

Use

Tags

6xHis

Expression

Mammalian

Mutation

K710,719R

Promoter

Available since

April 21, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-OSF-PP-human-CHMP3(150)

Plasmid

#154181

Purpose

expresses human CHMP3(150) in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

Insert

CHMP3 (CHMP3 Human)

Use

Tags

FLAG and Strep-Tag II

Expression

Mammalian

Mutation

C-terminal truncation of CHMP3 at amino acid 150

Promoter

CAG

Available since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pLV_TRET_Ngn2‐2A‐Ngn1

Plasmid

#61471

Purpose

3rd generation lentiviral vector; TetON promoter driving mouse Ngn2 and Ngn1

Depositor

Ron Weiss

Article

Busskamp et al Mol Syst Biol. 2014 Nov 17;10(11):760. d

Inserts

Ngn2 (Neurog2 Mouse)
Neurog1 (Neurog1 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 2, 2015

Availability

Academic Institutions and Nonprofits only

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