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Plasmid#224530PurposeA Gateway compatible 3' entry clone containing a V5 tagged mClover3 followed by a polyADepositorInsertV5-mClover3-pA
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
p3E-IRES-mTagBFP2-pA (JDW 1361)
Plasmid#224535PurposeA Gateway compatible 3' entry clone containing an IRES-FLAG-NLS-mTagBFP2-pADepositorInsertIRES-FLAG-NLS-mTagBFP2-pA
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
p3E-IRES-(n2)StayGold(c4)-pA (JDW 1364)
Plasmid#224537PurposeA Gateway compatible 3' entry clone containing IRES-(n2)StayGold(c4)-pADepositorInsertIRES-(n2)StayGold(c4)-pA
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-Lifeact- mScarlet-HA (JDW 1247)
Plasmid#224538PurposeA Gateway compatible middle entry clone containing Lifeact-mScarlet-I (far red f-actin reporter)DepositorInsertLifeact-mScarlet-HA
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME H2B mCerulean (JDW 1150)
Plasmid#224543PurposeA Gateway compatible middle entry clone containing a histone H2B fusion to mCerulean to label the nucleusDepositorInsertH2B-mCerulean
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEY142
Plasmid#223489Purposeunc-17(3k) fluorescent neural reporter driving nuclear mCyOFP1 expression (refer to NeuroPAL paper for expression)DepositorInsertunc-17(3k) (unc-17 Nematode)
UseTagsExpressionWormMutationPromoterAvailable sinceSept. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEY136
Plasmid#223483Purposetag-168 fluorescent neural reporter driving nuclear TagRFP-T expression (refer to NeuroPAL paper for expression)DepositorInserttag-168
UseTagsExpressionWormMutationPromoterAvailable sinceSept. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEY120
Plasmid#223467Purposesra-11(pB) fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)DepositorInsertsra-11(pB) (sra-11 Nematode)
UseTagsExpressionWormMutationPromoterAvailable sinceSept. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEY118
Plasmid#223465Purposeser-2(prom3) fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)DepositorInsertser-2(prom3) (ser-2 Nematode)
UseTagsExpressionWormMutationPromoterAvailable sinceSept. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEY114
Plasmid#223461Purposepdf-1 fluorescent neural reporter driving nuclear CyOFP1 expression (refer to NeuroPAL paper for expression)DepositorInsertpdf-1 (pdf-1 Nematode)
UseTagsExpressionWormMutationPromoterAvailable sinceSept. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEY110
Plasmid#223457Purposeodr-10 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)DepositorInsertodr-10 (odr-10 Nematode)
UseTagsExpressionWormMutationPromoterAvailable sinceSept. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEY100
Plasmid#223447Purposelad-2 fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)DepositorInsertlad-2 (lad-2 Nematode)
UseTagsExpressionWormMutationPromoterAvailable sinceSept. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEY76
Plasmid#223445Purposedop-2 fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)DepositorInsertdop-2 (dop-2 Nematode)
UseTagsExpressionWormMutationPromoterAvailable sinceSept. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEY74
Plasmid#223444Purposecho-1(3.1-2.6k) fluorescent neural reporter driving nuclear CyOFP1 expression (refer to NeuroPAL paper for expression)DepositorInsertcho-1(3.1-2.6k) (cho-1 Nematode)
UseTagsExpressionWormMutationPromoterAvailable sinceSept. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
lentiMutB3GALT5-blast
Plasmid#208381Purposelentiviral vector for expressing human B3GALT5 with C-terminal myc-DDK tag, includes nucleotide change in PAM targeting sequenceDepositorInsertbeta-1,3-galactosyltransferase 5 (B3GALT5 Human)
UseLentiviralTagsmyc, FLAGExpressionMammalianMutationnucleotide change in PAM targeting sequence nt 51…PromoterEF-1 alpha core promoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBK1289-AAV-dCjCas9
Plasmid#223138PurposeAAV backbone for dCjCas9 (endonuclease dead Cas9 from Campylobacter jejuni) with Cj-gRNA scaffoldDepositorTypeEmpty backboneUseAAV and CRISPRTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPU-HSA-miR30-MDA5
Plasmid#174222PurposeAll-in-one control rescue-MDA5 shRNA knockdown vectorDepositorInsertHSA
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterSFFVAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBK1290-AAV-dSaCas9
Plasmid#223139PurposeAAV backbone for dSaCas9 (endonuclease dead Cas9 from Staphylococcus aureus) and Sa-gRNA ScaffoldDepositorTypeEmpty backboneUseAAV and CRISPRTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEY139
Plasmid#223486Purposetph-1 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)DepositorInserttph-1 (tph-1 Nematode)
UseTagsExpressionWormMutationPromoterAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEY135
Plasmid#223482Purposetab-1 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)DepositorInserttab-1 (tab-1 Nematode)
UseTagsExpressionWormMutationPromoterAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only