Addgene

We narrowed to 24,726 results for: sta

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UKNP5.1.1

Plasmid

#98271

Purpose

Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

Depositor

Jonathan Ball

Article

Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

Insert

HCV E1E2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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UKNP4.1.1

Plasmid

#98266

Purpose

Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

Depositor

Jonathan Ball

Article

Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

Insert

HCV E1E2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/TO-p97wt-mycStrep

Plasmid

#31837

Depositor

Hemmo Meyer

Article

Ritz et al Nat Cell Biol. 2011 Aug 7;13(9):1116-23.

Insert

p97

Use

Tags

Strep and myc

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV

Available since

Oct. 17, 2011

Availability

Academic Institutions and Nonprofits only
pLL3.7 shRNA against CRTC2

Plasmid

#72711

Purpose

Lentiviral expression of shRNA against CRTC2 (3rd generation)

Depositor

Freddy Jeanneteau

Article

Jeanneteau et al Proc Natl Acad Sci U S A. 2012 Jan 24;10

Insert

CRTC2

Use

Lentiviral

Tags

CMV-EGFP

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA5FRT/TO-p97-EQ-mycStrep

Plasmid

#31838

Depositor

Hemmo Meyer

Article

Ritz et al Nat Cell Biol. 2011 Aug 7;13(9):1116-23.

Insert

p97

Use

Tags

Strep and myc

Expression

Bacterial and Mammalian

Mutation

E578Q

Promoter

CMV

Available since

Oct. 17, 2011

Availability

Academic Institutions and Nonprofits only
pRJPaph-gusA

Plasmid

#67036

Purpose

Plasmid for Paph-gusA integration downstream of B. diazoefficiens (B. japonicum) scoI

Depositor

Julia Vorholt

Article

Ledermann et al Mol Plant Microbe Interact. 2015 Jun 2.

Inserts

B. diazoefficiens DNA from scoI downstream region (comprising blr1132')
gusA

Use

Conjugative bacterial vector

Tags

Expression

Mutation

Promoter

Available since

Oct. 30, 2015

Availability

Academic Institutions and Nonprofits only

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PGRMC2-CD4d3+4-bio

Plasmid

#73112

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding PGRMC2 with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

PGRMC2 (PGRMC2 Human)

Use

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

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GFP-PIWI

Plasmid

#11591

Depositor

Edward Chan

Article

Jakymiw et al Nat Cell Biol. 2005 Dec . 7(12):1267-74.

Insert

Ago2 (AGO2 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

contain only aa482-869

Promoter

Available since

June 30, 2006

Availability

Academic Institutions and Nonprofits only
pK RagA(Q66L)-RagC

Plasmid

#99700

Purpose

Bacterial expression of RagA(Q66L)-RagC

Depositor

Kuang Shen, David Sabatini

Article

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Inserts

RagA (RRAGA Human)
RagC (RRAGC Human)

Use

Tags

His tag, Poly Arg, and SUMO

Expression

Bacterial

Mutation

L91P

Promoter

T7

Available since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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mOrange2-Tubulin-6

Plasmid

#57974

Purpose

Localization: Microtubules, Excitation: 549, Emission: 565

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Insert

Tubulin (TUBA1B Human)

Use

Tags

mOrange2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only
pET28a-MBP-CBX1-eReader

Plasmid

#135089

Purpose

Bacterial expression of engineered CBX1 with enhanced binding to H3K9me

Depositor

Marcey Waters

Article

Albanese et al ACS Chem Biol. 2020 Jan 17;15(1):103-111

Insert

CBX1 chromobox protein 1, engineered

Use

Tags

6xhis-tag and MBP

Expression

Bacterial

Mutation

lysine 43 mutated to alanine; aspartic acid 59 mu…

Promoter

T7

Available since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pAC-mCherry2-G4

Plasmid

#164167

Purpose

Expresses mCherry2 under the control of CMV promoter, basic vector for AAV, pAAV-CMV backbone, multiclonig site at C terminal

Depositor

Isei Tanida

Article

Tanida et al Sci Rep. 2020 Dec 14;10(1):21871. doi: 1

Insert

mCherry2

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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pMOD_C2813

Plasmid

#91092

Purpose

Module C, Promoter: PvUbi2, Gene: Csy4 (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Csy4 (+ BaeI site for GT donor cloning)

Use

CRISPR

Tags

Expression

Mutation

Promoter

PvUbi2

Available since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA-FLAG-Bbs4

Plasmid

#128868

Purpose

For mammalian expression of mouse Bbs4

Depositor

Aimin Liu

Article

Ye et al Proc Natl Acad Sci U S A. 2014 Feb 11;11

Insert

Bbs4 (Bbs4 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pDY-HA1-HA2-neo

Plasmid

#109123

Purpose

FR cassette provider to introduce FR cassette to a BAC by recombineering

Depositor

David Gilbert

Article

Sima et al Nucleic Acids Res. 2018 Feb 28;46(4):181

Inserts

HA1
HA2

Use

Tags

Expression

Mutation

Promoter

Available since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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pRS-Dhh1-PA(D195A,E196A,S226A,T228A)(405)

Plasmid

#122067

Purpose

Expressing Dhh1-PA

Depositor

Daniel Klionsky

Article

Liu et al PLoS Biol. 2019 Apr 11;17(4):e3000219. d

Insert

Dhh1-PA (DHH1 Budding Yeast)

Use

Tags

PA

Expression

Yeast

Mutation

Aspartic acid 195 to alanine; Glutamic acid 196 t…

Promoter

Available since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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pIRESpuro2-UBXD1-3x HA

Plasmid

#31836

Depositor

Hemmo Meyer

Article

Ritz et al Nat Cell Biol. 2011 Aug 7;13(9):1116-23.

Insert

UBXD1 (UBXN6 Human)

Use

Tags

3x HA

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV

Available since

Oct. 17, 2011

Availability

Academic Institutions and Nonprofits only
pMOD_A0601

Plasmid

#91016

Purpose

Module A, Promoter: 35S, Gene: Csy4-P2A-AtCas9_D10A nickase, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Csy4-P2A-AtCas9_D10A nickase

Use

CRISPR

Tags

Expression

Mutation

D10A

Promoter

35S

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pSBL_DK186

Plasmid

#156422

Purpose

Mammalian expression of 3a-EGFP with CMV promoter, can be used for transfection or used to generate bacmids for baculoviral infection of mammalian cells

Depositor

Stephen Brohawn

Article

Kern et al bioRxiv [Preprint]. 2021 Jan 26:2020.06.

Insert

SARS-CoV-2 3a protein

Use

Tags

EGFP-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR221 Cas9-RFC5

Plasmid

#183200

Purpose

Entry clone

Depositor

Emma Haapaniemi

Article

Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

Insert

Cas9-RFC5

Use

CRISPR; Gateway entry clone

Tags

Expression

Mutation

n/a

Promoter

Available since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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