Skip to main content
Addgene
Showing: 9721 - 9740 of 13607 results

  6. Fibronectin-human-mutated V1 O-Glycosylation site

    Plasmid
    #120406
    Purpose
    enables eukaryotic expression of human plasma fibronectin in which the third O-glycosylation site was mutated from threonine to serine
    Depositor
    Inaam Nakchbandi
    Article
    Sens et al J Bone Miner Res. 2017 Jan;32(1):70-81.
    Insert
    Fibronectin (FN1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Contains complete variable region with a mutation…
    Promoter
    CMV
    Available since
    Jan. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    120406-plasmid-map-sequence-id-233165

    Enlarge

  7. Fibronectin-human-mutated N-terminal (N), V1 and V2 Glycosylation sites

    Plasmid
    #120407
    Purpose
    enables eukaryotic expression of human plasma fibronectin in which all three O-glycosylation sites were mutated from threonine to serine
    Depositor
    Inaam Nakchbandi
    Article
    Sens et al J Bone Miner Res. 2017 Jan;32(1):70-81.
    Insert
    Fibronectin (FN1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Contains complete variable region with mutations …
    Promoter
    CMV
    Available since
    Jan. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    120407-plasmid-map-sequence-id-232461

    Enlarge

  8. pTT3-ecdCD80-ST3-His

    Plasmid
    #210665
    Purpose
    Expression of the extracellular domain of human CD80 fused to Spytag003 + Histag in HEK293 (or similar)
    Depositor
    Omer Dushek
    Article
    Patel et al EMBO J. 2024 Jan;43(1):132-150. doi: 10.
    Insert
    Extracellular domain of human CD80 fused to Spytag003 and Histag (CD80 Human)
    Use
    Tags
    Histag and Spytag003
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    210665-plasmid-map-sequence-id-443131

    Enlarge

  13. pBabePuro-FLAG-KrascomQ61R

    Plasmid
    #206863
    Purpose
    To stably express mouse Kras encoded by common mouse codons (to increase expression) and with a Q61R mutation with an N-terminal FLAG tag in cells resistant to Neo.
    Depositor
    Christopher Counter
    Article
    Le Roux et al Elife. 2022 Sep 7;11:e75715. doi: 10.755
    Insert
    Kras (Kras Mouse)
    Use
    Retroviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Codons 1-3 altered to most optimum based on mouse…
    Promoter
    Available since
    April 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    206863-plasmid-map-sequence-id-430070

    Enlarge

  14. pBabePuro-FLAG-KrascomG12D

    Plasmid
    #206862
    Purpose
    To stably express mouse Kras encoded by common mouse codons (to increase expression) and with a G12D mutation with an N-terminal FLAG tag in cells resistant to Neo..
    Depositor
    Christopher Counter
    Article
    Le Roux et al Elife. 2022 Sep 7;11:e75715. doi: 10.755
    Insert
    Kras (Kras Mouse)
    Use
    Retroviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Codons in exons 1-3 altered to most optimum based…
    Promoter
    Available since
    April 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    206862-plasmid-map-sequence-id-430069

    Enlarge

  15. pAAV2-UbC-3xNLS-mScarlet-I

    Plasmid
    #191099
    Purpose
    To express a bright monomeric red FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methods
    Depositor
    Murray Blackmore, Pantelis Tsoulfas
    Article
    Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:
    Insert
    3xNLS-mScarlet-I
    Use
    AAV and Synthetic Biology
    Tags
    Three repeats of the nuclear localizzation seque…
    Expression
    Mammalian
    Mutation
    optimized to human codon usage
    Promoter
    UbC
    Available since
    Dec. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    191099-plasmid-map-sequence-id-380113

    Enlarge

  19. pCEP4-myc-PDGFRA-D842V_EGFP-PEST_reporter

    Plasmid
    #172598
    Purpose
    Mammalian fluorescent (EGFP-PEST) reporter plasmid for PDGFRA-D842V mutant signaling.
    Depositor
    Daniel Kiss
    Article
    Yu et al Hum Mol Genet. 2022 Jul 21;31(14):2348-2
    Insert
    myc-PDGFRA-D842V (PDGFRA Human, Synthetic)
    Use
    Egfp-pest_reporter
    Tags
    Extracellular c-myc epitope tag and Signal peptid…
    Expression
    Mammalian
    Mutation
    PDGFRA-D842V
    Promoter
    CMV
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    172598-plasmid-map-sequence-id-338911

    Enlarge

  20. pCEP4-myc-PDGFRA-D842V+R914W_EGFP-PEST_reporter

    Plasmid
    #172599
    Purpose
    Mammalian fluorescent (EGFP-PEST) reporter plasmid for PDGFRA-D842V+R914W mutant signaling.
    Depositor
    Daniel Kiss
    Article
    Yu et al Hum Mol Genet. 2022 Jul 21;31(14):2348-2
    Insert
    myc-PDGFRA-D842V+R914W (PDGFRA Human, Synthetic)
    Use
    Egfp-pest_reporter
    Tags
    Extracellular c-myc epitope tag and Signal peptid…
    Expression
    Mammalian
    Mutation
    PDGFRA-D842V+R914W
    Promoter
    CMV
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    172599-plasmid-map-sequence-id-338914

    Enlarge

Showing: 9721 - 9740 of 13607 results