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Plasmid#110152PurposepSKB3 with TEV-cleavable, N-terminal His-tagged SaGGR-I206F-L377H mutant geneDepositorInsertGGR
UseTagsTEV-cleavable His-tagExpressionBacterialMutationSaGGR-I206F-L377H mutant genePromoterT7Available sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pet28a_RBPMS_FL
Plasmid#59391PurposePlasmid for bacterial overexpression of RBPMSDepositorInsertRBPMS (RBPMS Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
FgH1tUTG_mup53_Ex4
Plasmid#85534PurposeInducible expression of guide RNA (mup53_Ex4) with fluorescent GFP reporterDepositorInsertmu p53
UseTagsExpressionMutationPromoterH1tAvailable sinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPK1E-2+t
Plasmid#48148PurposeShuttle vector E. coli/B.meg., encoding a promoter and a terminator for RNA polymerase of the phage K1EDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCR4_SNORD27 probe
Plasmid#73069Purposeantisense probe for detection of human SNORD27DepositorInsertSNORD27 (SNORD2 Human)
UseIn vitro transcriptionTagsExpressionMutationPromotert7Available sinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pET22b-ecDHFR-Y100F
Plasmid#110826PurposeBacterial expression of E.coli DHFR Y100F mutantDepositorInsertecDHFR
UseTagsExpressionBacterialMutationY100 mutated to F100PromoterT7Available sinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pQTEV-TCTEL1
Plasmid#31337DepositorInsertt-complex-associated-testis-expressed 1-like 1 (DYNLT1 Human)
UseTagsHis and TEVExpressionBacterialMutationPromoterAvailable sinceNov. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDOE11 mas:mTq2 transient
Plasmid#124248PurposeAllows bimolecular fluorescence complementation via transient expression of two proteins in directly transfected plant cells.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSUPER-ARF4b
Plasmid#67498PurposeExpression of shRNA targeting human ARF4bDepositorInsertHsARF4 shRNA (ARF4 Human)
UseRNAiTagsExpressionMammalianMutationPromoterH1Available sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
PcDNA-DEST53-CypB-Nucl N-term GFP
Plasmid#36123DepositorInsertcyclophilin B (PPIB Human, contains CypB AA 33-216)
UseTagsGFPExpressionMammalianMutationA212SPromoterCMVAvailable sinceNov. 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046831
Plasmid#145671PurposeBacterial Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymeraseDepositorInsertSARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;MycExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046970
Plasmid#145753PurposeBacterial Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)DepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;HAExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pQTEV-DGKA
Plasmid#31294DepositorInsertdiacylglycerol kinase, alpha (DGKA Human)
UseTagsHis and TEVExpressionBacterialMutationPromoterAvailable sinceMarch 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR - Amplicon, BCR/ABL sgRNA 2
Plasmid#70658PurposeExpresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an intergenic BCR/ABL-targeting sgRNA element from U6 promoter. Lentiviral backbone.DepositorInsertsgRNA against BCR/ABLamplicon found in CML-derived K562 cell line
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET3a pro-hRegIIIa
Plasmid#64935Purposebacterial expression of full length human RegIIIalphaDepositorInsertRegIIIa (REG3A Human)
UseTagsExpressionBacterialMutationmutations made in 5' end to relax secondary …PromoterT7Available sinceJune 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCRII-Topo Cux2 in situ probe
Plasmid#45623DepositorInsertCux2 in situ probe (Cux2 Mouse)
UseIn situTagsExpressionMutationfragment contains nt 1069-1694 on NM_007804PromoterAvailable sinceJune 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS_AKAR5’
Plasmid#114141PurposeMammalian expression of the AKAR5’ sensor for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)DepositorInsertAKAR5'
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 4, 2018AvailabilityAcademic Institutions and Nonprofits only