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Showing: 9621 - 9640 of 28090 results

  1. pHBJT003

    Plasmid
    #225154
    Purpose
    Medium copy cloning vector for integration of C-terminal FLAG epitope tag. SmaI restriction site immediately upstream of the FLAG epitope. FLAG epitope in same orientation as lac promoter.
    Depositor
    Jenny-Lee Thomassin
    Article
    Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    FLAG epitope tag (35 bp)
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    225154-plasmid-map-sequence-id-444670

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  2. pALS3-Ma-SUMO-sfGFP-WT

    Plasmid
    #212122
    Purpose
    Fluorescence control plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses SUMO-sfGFP-WT and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.
    Depositor
    Ryan Mehl
    Inserts
    SUMO-sfGFP-WT
    M. alvus Pyl-tRNA(6)
    Use
    Tags
    His6 and SUMO
    Expression
    Bacterial
    Mutation
    Promoter
    araC and lpp
    Available since
    Sept. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    212122-plasmid-map-sequence-id-444187

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  3. pALS3-Ma-sfGFP-TAG150

    Plasmid
    #212121
    Purpose
    Fluorescence plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-TAG150 and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.
    Depositor
    Ryan Mehl
    Inserts
    sfGFP-150TAG
    M. alvus Pyl-tRNA(6)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    N150TAG
    Promoter
    araC and lpp
    Available since
    Sept. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    212121-plasmid-map-sequence-id-444186

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  15. pPRISM-QF2-he1.1:YFP

    Plasmid
    #184819
    Purpose
    Modified version of GeneWeld method from Wierson et al., 2020 (https://doi.org/10.7554/eLife.53968). Backbone into which homology arms are cloned for CRISPR/Cas9-mediated integration of QF2.
    Depositor
    Marnie Halpern
    Article
    Spikol et al eLife 12:RP89516
    Insert
    QF2-pA-he1.1:YFP
    Use
    CRISPR; Backbone for cloning of homology arms (fo…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    184819-plasmid-map-sequence-id-366121

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  16. pPRISM-QF2-he1.1:YFP-rln3a-HA

    Plasmid
    #184820
    Purpose
    Modified version of GeneWeld method from Wierson et al., 2020 (https://doi.org/10.7554/eLife.53968). Contains homology arm inserts for integration of QF2 into rln3a gene by CRISPR/Cas9 integration.
    Depositor
    Marnie Halpern
    Article
    Spikol et al eLife 12:RP89516
    Insert
    rln3a-HA
    Use
    CRISPR; For integration of qf2 by homology-direct…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    184820-plasmid-map-sequence-id-366119

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  17. TOPO-nmba

    Plasmid
    #184821
    Purpose
    Used to synthesize RNA probe for in situ hybridization for nmba
    Depositor
    Marnie Halpern
    Article
    Spikol et al eLife 12:RP89516
    Insert
    nmba (nmba Zebrafish)
    Use
    Template for synthesis of nmba probe
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    184821-plasmid-map-sequence-id-366113

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  18. dSp-Rta 125-175

    Plasmid
    #99668
    Purpose
    Expresses dSp Cas9 fused to truncated RTA activation domain
    Depositor
    George Church
    Article
    Vora et al bioRxiv 2018. 298620
    Insert
    dCas9
    Use
    Tags
    truncated RTA activation domain (125-175)
    Expression
    Mammalian
    Mutation
    dead Cas9
    Promoter
    Available since
    May 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    99668-plasmid-map-sequence-id-189832

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  20. dSp-p65 (1-100)

    Plasmid
    #99660
    Purpose
    Expresses dSp Cas9 fused to truncated p65 activation domain
    Depositor
    George Church
    Article
    Vora et al bioRxiv 2018. 298620
    Insert
    dCas9
    Use
    Tags
    truncated p65 activation domain (1-100)
    Expression
    Mammalian
    Mutation
    dead Cas9
    Promoter
    Available since
    May 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    99660-plasmid-map-sequence-id-189821

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Showing: 9621 - 9640 of 28090 results