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MAC_N_MED13
Plasmid#111689PurposeMAC-tagged gene expressionDepositorInsertMED13 (MED13 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
tetO-SOX9
Plasmid#170703Purposedoxycycline-inducible overexpression of SOX9DepositorInsertSOX9 (SOX9 Human)
UseLentiviral; Doxycycline inducibleTagsExpressionMammalianMutationPromoterTRE promoter, Tet-ONAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
delCMV-mCherry-3x-p67phox-GBD
Plasmid#214141PurposeRac activity sensorDepositorInsert3xp67phox-GBD (NCF2 Human)
UseTagsmCherryExpressionMammalianMutationOnly the GTPase binding domain (GBD) is includedPromoterdelCMVAvailable sinceApril 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
HSC1-HS4-D4Z4c-GiP
Plasmid#58541PurposeDerived from HSC1-GiP where 2 copies of the HS4 insulator core inside the 3'LTR and the D4Z4-c insulator, are combined upstream of the EF1 alpha promoterDepositorInsertsEnhanced Green Fluorescence Protein, Puromycin resistance gene
D4Z4 repeat, partial
UseRetroviralTagsExpressionMammalianMutationPromoterHuman EF1-alphaAvailable sinceSept. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmeIF4G-V5His6
Plasmid#79259PurposeExpresses lambdaN-HA-tagged and C-terminal V5-His6-tagged DmeIF4G in S2 cellsDepositorInsertDmeIF4G (eIF4G1 Fly)
UseSchneider 2 cell expressionTagsV5-His6 and lambdaN-HAExpressionMutationPromoterAc5 promoterAvailable sinceJuly 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
MAC_C_CH60
Plasmid#111662PurposeMAC-tagged gene expressionDepositorInsertCH60 (HSPD1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
MAC_C_HXK1
Plasmid#111668PurposeMAC-tagged gene expressionDepositorInsertHXK1 (HK1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
PA-mCherry1-Mito-7
Plasmid#57189PurposeLocalization: Mitochondria, Excitation: 400 / 504, Emission: 515 / 517DepositorInsertMito (COX8A Human)
UseTagsPA-mCherry1ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
Mucolipin1 D471-472K-pHcRed C1
Plasmid#62961Purposefull length human Mucolipin-1 D471/472K cloned into pHcRed1 C1DepositorInsertMucolipin-1 (MCOLN1 Human)
UseTagsHcRedExpressionMammalianMutationD471/472K (and two silent mutations T77 ACA inst…PromoterCMVAvailable sinceMay 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
p20StAD
Plasmid#190268PurposeConstitutively expresses a protein of interest fused to a GAL4 transcription activating domain. Targeted integration to genomic locus YPRC-delta15, Chromosome XVI.DepositorTypeEmpty backboneUseSynthetic Biology; Yeast genome-integrating vectorTagsGAL4-ADExpressionMutationPromoterTDH3Available sinceNov. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pH2R-P2A-mCherry-N1
Plasmid#84331PurposeCo-expresses (untagged) Histamine-2 receptor and mCherryDepositorInsertH2R-P2A-mCherry (HRH2 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
Venus-G protein Beta2 in pcDNA3.1
Plasmid#42182DepositorInsertG protein Beta 2 (GNB2 Human)
UseTagsVenus(156-239)ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
Kif1A(1-489)-DHFR-myc
Plasmid#117833PurposeConstitutively active Kinesin-3 motor tagged with DHFR and mycDepositorInsertKif1A (1-489) (Kif1a Mouse)
UseTagsDHFR and mycExpressionMutationonly aa 1-489 (motor domain)PromoterCMVAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEX-CMV-SP-YFP-STM2(15-746) EF hand D80A
Plasmid#18864DepositorInsertStromal interaction molecule 2 (STIM2 Human)
UseTagsYFPExpressionMammalianMutationEF hand mutant: D80A. YFP inserted between signal…PromoterAvailable sinceMarch 24, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT/TO-PLAA-Strep-HA
Plasmid#113484PurposeExpression of human PLAA with C-terminal strep-HA tagDepositorInsertPLAA (PLAA Human)
UseTags2xstrep-HAExpressionMammalianMutationwildtype without stop codonPromoterCMVAvailable sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
MAC_C_AIFM1
Plasmid#111659PurposeMAC-tagged gene expressionDepositorInsertAIFM1 (AIFM1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
CAG-Brn2-DBD-EnR
Plasmid#19714DepositorUseTagsExpressionMammalianMutationmouse Brn2 DNA binding domain-Drosophila engraile…PromoterAvailable sinceOct. 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
pFS_1061_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)-3xTerm_GGC(BbsI)-Leader-ARRAY2-DR2-FaqI_Term_NotI
Plasmid#184738Purposeencodes aTc inducible FsRT-Cas1–Cas2 and transcriptionally insulated FsCRISPR Array 2DepositorInsertFsRT-Cas1(opt)-Cas2(opt)
UseTagsExpressionBacterialMutationsequence codon optimized for expression in E. coliPromoterpTetAAvailable sinceMay 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAC147-pCR8-dCas9VP64
Plasmid#48219PurposedCas9VP64 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expressionDepositorInsertdCas9(D10A;H840A) fusion with VP64 activation domain
UseCRISPR; Gateway donorTagsHA Tag and VP64ExpressionMutationD10A;H840APromoterAvailable sinceSept. 17, 2013AvailabilityAcademic Institutions and Nonprofits only
