Addgene

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R5 MCS

Plasmid

#51732

Purpose

Dicistronic vector with cloning site

Depositor

Vincent Mauro

Article

Burman et al Nucleic Acids Res. 2012 Sep;40(16):8085-

Insert

MCS

Use

Tags

Expression

Mutation

Promoter

SV40

Available since

June 12, 2014

Availability

Academic Institutions and Nonprofits only

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pTU-A-005

Plasmid

#124412

Purpose

Slot A, contains mKO2-MarA (step 1)

Depositor

Yolanda Schaerli

Article

Santos-Moreno et al ACS Synth Biol. 2019 Jul 19;8(7):1691-16

Insert

slot A with mKO2-MarA reporter + terminator + spacer

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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pTfdc1Sc

Plasmid

#78287

Purpose

Expresses S. cerevisiae FDC1 in E. coli

Depositor

David Nielsen

Article

McKenna et al Metab Eng. 2011 Sep;13(5):544-54. doi: 1

Insert

FDC1 (FDC1 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

Promoter

trc

Available since

June 28, 2016

Availability

Academic Institutions and Nonprofits only

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pTU-B-005

Plasmid

#124413

Purpose

Slot B, contains sfGFP-MarAn20 (step 1)

Depositor

Yolanda Schaerli

Article

Santos-Moreno et al ACS Synth Biol. 2019 Jul 19;8(7):1691-16

Insert

slot B with sfGFP-MarAn20 reporter + terminator + spacer

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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pTU-C-005

Plasmid

#124414

Purpose

Slot C, contains RepA70-mKate2 (step 1)

Depositor

Yolanda Schaerli

Article

Santos-Moreno et al ACS Synth Biol. 2019 Jul 19;8(7):1691-16

Insert

slot C with RepA70-mKate2 reporter + terminator

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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pTU-B-000

Plasmid

#124410

Purpose

Slot B, empty (step 1)

Depositor

Yolanda Schaerli

Article

Santos-Moreno et al ACS Synth Biol. 2019 Jul 19;8(7):1691-16

Insert

slot B + terminator + spacer

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTU-C-000

Plasmid

#124411

Purpose

Slot C, empty (step 1)

Depositor

Yolanda Schaerli

Article

Santos-Moreno et al ACS Synth Biol. 2019 Jul 19;8(7):1691-16

Insert

slot C + terminator

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMAL-c2G

Plasmid

#75290

Purpose

Expresses proteins in the cytoplasm as fusions to maltose-binding protein

Depositor

Paul Riggs

Article

Walker et al Appl Microbiol Biotechnol. 2010 Sep;88(1

Type

Empty backbone

Use

Tags

maltose-binding protein

Expression

Bacterial

Mutation

Promoter

tac

Available since

July 20, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pJK285

Plasmid

#71551

Purpose

Production of Acetobacter aceti 1023 alanine racemase (alr) with N-terminal His tag (H6AaAlr)

Depositor

T. J. Kappock

Article

Francois et al Protein Expr Purif. 2007 Jan;51(1):39-48

Insert

alanine racemase

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pHIS-Hsv2

Plasmid

#42524

Depositor

James Hurley

Article

Baskaran et al Mol Cell. 2012 Aug 10;47(3):339-48. doi:

Insert

Hsv2

Use

Tags

HIS-TEV

Expression

Bacterial

Mutation

Promoter

Available since

July 25, 2013

Availability

Academic Institutions and Nonprofits only
Dendra-SpVasa

Plasmid

#185583

Purpose

for photoconversion

Depositor

Mamiko Yajima

Article

Wavreil et al Dev Biol. 2021 Oct;478:13-24. doi: 10.10

Insert

Vssa

Use

In vitro transcription (mrna synthesis)

Tags

Dendra

Expression

Mutation

Promoter

Available since

Oct. 6, 2022

Availability

Academic Institutions and Nonprofits only

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Kaede-SpVasa

Plasmid

#185582

Purpose

for photoconversion

Depositor

Mamiko Yajima

Article

Wavreil et al Dev Biol. 2021 Oct;478:13-24. doi: 10.10

Insert

Vasa

Use

In vitro transcription (mrna synthesis)

Tags

Kaede

Expression

Mutation

Promoter

Available since

Oct. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pSP64-mCherry

Plasmid

#135597

Purpose

Expression of mCherry mRNA for microinjection into S. purpuratus

Depositor

Mamiko Yajima

Article

Uchida et al Dev Biol. 2018 Sep 1;441(1):19-30. doi:

Insert

mCherry

Use

In vitro transcription (mrna synthesis)

Tags

Expression

Mutation

Promoter

Unknown

Available since

Jan. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pTU-B-013

Plasmid

#124416

Purpose

Slot B, empty (step 1)

Depositor

Yolanda Schaerli

Article

Santos-Moreno et al ACS Synth Biol. 2019 Jul 19;8(7):1691-16

Insert

slot B + terminator + spacer

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTU-C-013

Plasmid

#124417

Purpose

Slot C, empty (step 1)

Depositor

Yolanda Schaerli

Article

Santos-Moreno et al ACS Synth Biol. 2019 Jul 19;8(7):1691-16

Insert

slot C + terminator

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTU-A-010

Plasmid

#124418

Purpose

Slot A, contains mKO2-MarA (step 1)

Depositor

Yolanda Schaerli

Article

Santos-Moreno et al ACS Synth Biol. 2019 Jul 19;8(7):1691-16

Insert

slot A with mKO2-MarA reporter + terminator + spacer

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTU-B-010

Plasmid

#124419

Purpose

Slot B, contains sfGFP-MarAn20 (step 1)

Depositor

Yolanda Schaerli

Article

Santos-Moreno et al ACS Synth Biol. 2019 Jul 19;8(7):1691-16

Insert

slot B with sfGFP-MarAn20 reporter + terminator + spacer

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTU-C-010

Plasmid

#124420

Purpose

Slot C, contains RepA70-mKate2 (step 1)

Depositor

Yolanda Schaerli

Article

Santos-Moreno et al ACS Synth Biol. 2019 Jul 19;8(7):1691-16

Insert

slot C with RepA70-mKate2 reporter + terminator

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pISH-Vmn1r168

Plasmid

#105995

Purpose

To prepare riboprobe for in situ hybridization

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

Vmn1r168 (Vmn1r168 Mouse)

Use

To generate riboprobe

Tags

Expression

Mutation

Promoter

Available since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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pJK288

Plasmid

#71552

Purpose

Production of Acetobacter aceti 1023 alanine racemase (alr) with Tyr260>Ala mutant (AaAlr-Y260A)

Depositor

T. J. Kappock

Article

Francois et al Protein Expr Purif. 2007 Jan;51(1):39-48

Insert

alanine racemase

Use

Tags

Expression

Bacterial

Mutation

changes tyrosine-260 to alanine

Promoter

Available since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge