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Plasmid#169749PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-868, P801G, T805C, C813S)-Strep.DepositorInsertGB1-His6-TEV-ALIX(703-868, P801G, G808C, C813S)-Strep (PDCD6IP Human)
UseTagsGB1-His6-TEV and Strep tag (WSHPQFEK)ExpressionBacterialMutationProline 801 to Glycine, Threonine 805 to Cysteine…PromoterAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
GB1-PRD(703-868, P801G, G808C, C813S)-Strep
Plasmid#169750PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-868, P801G, G808C, C813S)-Strep.DepositorInsertGB1-His6-TEV-ALIX(703-868, P801G, G808C, C813S)-Strep (PDCD6IP Human)
UseTagsGB1-His6-TEV and Strep tag (WSHPQFEK)ExpressionBacterialMutationProline 801 to Glycine, Glycine 808 to Cysteine, …PromoterAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
GB1-PRD(703-868, S712C, P801G, C813S)-Strep
Plasmid#164445PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-868, S712C, P801G, C813S)-Strep.DepositorInsertGB1-His6-TEV-ALIX(703-868, S712C, P801G, C813S)-Strep (PDCD6IP Human)
UseTagsGB1-His6-TEV and Strep tag (WSHPQFEK)ExpressionBacterialMutationSerine 712 to Cysteine, Proline 801 to Glycine, C…PromoterAvailable sinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
GB1-PRD(703-868, A756C, P801G, C813S)-Strep
Plasmid#164446PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-868, A756C, P801G, C813S)-Strep.DepositorInsertGB1-His6-TEV-ALIX(703-868, A756C, P801G, C813S)-Strep (PDCD6IP Human)
UseTagsGB1-His6-TEV and Strep tag (WSHPQFEK)ExpressionBacterialMutationAlanine 756 to Cysteine, Proline 801 to Glycine, …PromoterAvailable sinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
GB1-PRD(703-868, P801G, C813S, S863C)-Strep
Plasmid#164447PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-868, P801G, C813S, S863C)-Strep.DepositorInsertGB1-His6-TEV-ALIX(703-868, P801G, C813S, S863C)-Strep (PDCD6IP Human)
UseTagsGB1-His6-TEV and Strep tag (WSHPQFEK)ExpressionBacterialMutationProline 801 to Glycine, Cysteine 813 to Serine, S…PromoterAvailable sinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEBTetDBl-CLIP-CPAP
Plasmid#136865PurposeMammalian expression of the centrosomal protein CENPJ N-terminally fused to CLIP-tagDepositorInsertCLIP-CENPJ (CENPJ Human, Synthetic)
UseTagsFLAG-tag / His-tagExpressionMammalianMutationPromoterCMV-TetO2Available sinceMarch 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEBTetD-SNAP-CPAP
Plasmid#136816PurposeMammalian expression of the centrosomal protein CENPJ N-terminally fused to SNAP-tagDepositorInsertSNAP-CENPJ (CENPJ Human, Synthetic)
UseTagsFLAG-tag / His-tagExpressionMammalianMutationPromoterCMV-TetO2Available sinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCR3-vsv-AuroraB L154A/H250Y
Plasmid#108489Purposeexpression of vsv-AuroraB Analog sensitive (L154A/H250Y)DepositorInsertAuroraB (AURKB Human)
UseTagsVSVExpressionMammalianMutationAnalog sensitive L154A/H250YPromoterCMVAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
CCDC-bio-His
Plasmid#53331PurposeExpresses full-length Coiled-coil domain-containing protein 109A precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertCCDC (MCU Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
G6f-bio-His
Plasmid#53342PurposeExpresses full-length Lymphocyte antigen 6 complex locus protein G6f precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertG6f (LY6G6F Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
DCBL-bio-His
Plasmid#51867PurposeExpresses full-length Discoidin, CUB and LCCL domain-containing protein 2 ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertDCBL (DCBLD2 Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
ESAM-bio-His
Plasmid#51751PurposeExpresses full-length Endothelial cell-selective adhesion molecule precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertESAM (ESAM Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
NKP9 pmRFP-N2 (719)
Plasmid#61999Purposemammalian expression of nuclear envelope transmembrane proteinDepositorInsertC17orf62 (C17orf62 Human)
UseTagsmRFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
APP-bio-His
Plasmid#51643PurposeExpresses full-length Amyloid beta A4 protein precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertAPP (APP Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
ITGB1
Plasmid#51920PurposeExpresses full-length Integrin beta-1 precursor ectodomain in mammalian cells. Stop codon before C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertITGB1 (ITGB1 Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationStop Codon at amino acid 728, before CD4, bio and…PromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCIBN-tagBFP-hLaminB1
Plasmid#103799PurposeBLInCR 'Localizer' construct that marks the nuclear lamina and is targeted by a PHR-tagged effector upon illumination with blue lightDepositorUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCIBN-EYFP-hLaminB1
Plasmid#103800PurposeBLInCR 'Localizer' construct that marks the nuclear lamina and is targeted by a PHR-tagged effector upon illumination with blue lightDepositorUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-SLC25A47_STOP
Plasmid#161149PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.DepositorInsertSLC25A47 (SLC25A47 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 21, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
CDK6 gRNA (BRDN0001145692)
Plasmid#75643Purpose3rd generation lentiviral gRNA plasmid targeting human CDK6DepositorInsertCDK6 (CDK6 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
CDK6 gRNA (BRDN0001146904)
Plasmid#75644Purpose3rd generation lentiviral gRNA plasmid targeting human CDK6DepositorInsertCDK6 (CDK6 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only